Recombinant Human Nodal Protein | R&D Systems 3218-ND-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Nodal Protein

catalog :

3218-ND-025

quantity :

25 ug

price :

453 USD

more info or order :

R&D Systems product webpage

citations: 15

Reference
Harpelunde Poulsen K, Nielsen J, Grønkær Toft B, Joensen U, Rasmussen L, Blomberg Jensen M, et al. Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours. BMC Cancer. 2020;20:349 pubmed publisher
Vermilyea S, Babinski A, Tran N, To S, Guthrie S, Kluss J, et al. In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets. Sci Rep. 2020;10:3447 pubmed publisher
Ray P, Chin A, Worley K, Fan J, Kaur G, Wu M, et al. Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping. Proc Natl Acad Sci U S A. 2018;115:E11568-E11577 pubmed publisher
Jørgensen A, Macdonald J, Nielsen J, Kilcoyne K, Perlman S, Lundvall L, et al. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis. Cell Rep. 2018;25:1924-1937.e4 pubmed publisher
Jiang Y, Li H, Wang Y, Tian T, He Y, Jin Y, et al. ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line. Oncol Lett. 2017;14:6955-6961 pubmed publisher
Ball C, Oppel F, Ehrenberg K, Dubash T, Dieter S, Hoffmann C, et al. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts. EMBO Mol Med. 2017;9:918-932 pubmed publisher
Lee G, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, et al. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO. J Cell Biol. 2016;215:705-718 pubmed
Fuerer C, Nostro M, Constam D. Nodal·Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation. J Biol Chem. 2014;289:17854-71 pubmed publisher
Esquivies L, Blackler A, Peran M, Rodriguez Esteban C, Izpisua Belmonte J, Booker E, et al. Designer nodal/BMP2 chimeras mimic nodal signaling, promote chondrogenesis, and reveal a BMP2-like structure. J Biol Chem. 2014;289:1788-97 pubmed publisher
Quail D, Zhang G, Findlay S, Hess D, Postovit L. Nodal promotes invasive phenotypes via a mitogen-activated protein kinase-dependent pathway. Oncogene. 2014;33:461-73 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Castonguay R, Werner E, Matthews R, Presman E, Mulivor A, Solban N, et al. Soluble endoglin specifically binds bone morphogenetic proteins 9 and 10 via its orphan domain, inhibits blood vessel formation, and suppresses tumor growth. J Biol Chem. 2011;286:30034-46 pubmed publisher
Herrera B, Inman G. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol. 2009;10:20 pubmed publisher
Watanabe K, Hamada S, Bianco C, Mancino M, Nagaoka T, Gonzales M, et al. Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. J Biol Chem. 2007;282:35772-86 pubmed
McLean A, D Amour K, Jones K, Krishnamoorthy M, Kulik M, Reynolds D, et al. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells. 2007;25:29-38 pubmed

product information

brand :

R&D Systems

master code :

3218-ND

SKU :

3218-ND-025

product name :

Recombinant Human Nodal Protein

unit size :

25 ug

description :

The Recombinant Human Nodal Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Nodal Protein, CF has been validated for the following applications: Bioactivity.

target :

Nodal

category :

Proteins and Enzymes

alt names :

BMP-16, MGC138230, nodal homolog, nodal homolog (mouse), nodal, mouse, homolog

buffer :

Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

conjugate :

Unconjugated

all available conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

theoretical molecular weight :

12.9 kDa (monomer)

gene symbol :

NODAL

details of functionality :

Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al . (2001) Mol. Cell. 7 :949. Approximately 100 ng/mL of Recombinant Human Nodal can effectively induce Smad2 phosphorylation.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

Q96S42

applications :

Bioactivity

source :

E. coli-derived Recombinant Human Nodal Protein

USD :

419

Jul-22 :

453 USD

sizes available :

25 ug

more info or order :

R&D Systems product webpage