Recombinant Rhesus Macaque IL-12 Protein, CF | R&D Systems 3216-RL-025/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Rhesus Macaque IL-12 Protein, CF

catalog :

3216-RL-025/CF

quantity :

25 ug

price :

468 USD

more info or order :

R&D Systems product webpage

citations: 3

Reference
Watanabe S, Fujino M, Saito Y, Ahmed N, Sato H, Sugimoto C, et al. Protective Immune Responses Elicited by Deglycosylated Live-Attenuated Simian Immunodeficiency Virus Vaccine Are Associated with IL-15 Effector Functions. J Immunol. 2020;205:1331-1344 pubmed publisher
Wonderlich E, Wu W, Normolle D, Barratt Boyes S. Macrophages and Myeloid Dendritic Cells Lose T Cell-Stimulating Function in Simian Immunodeficiency Virus Infection Associated with Diminished IL-12 and IFN-Î± Production. J Immunol. 2015;195:3284-92 pubmed publisher
Li H, Richert Spuhler L, Evans T, Gillis J, Connole M, Estes J, et al. Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection. PLoS Pathog. 2014;10:e1004551 pubmed publisher

product information

master code :

3216-RL/CF

SKU :

3216-RL-025/CF

product name :

Recombinant Rhesus Macaque IL-12 Protein, CF

unit size :

25 ug

description :

The Recombinant Rhesus Macaque IL-12 Protein, CF from R&D Systems is derived from CHO. The Recombinant Rhesus Macaque IL-12 Protein, CF has been validated for the following applications: Bioactivity.

target :

IL-12

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Rhesus Macaque

observed molecular weight :

40-45 kDa and 36-37 kDa, reducing conditions

theoretical molecular weight :

35.6 kDa (p40), 23.4 kDa (p35)

details of functionality :

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. etáal . (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. etáal . (eds): IRL Press. 272. The ED50 for this effect is 0.07-0.3 ng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P48095 (p40) NP_001038199 (p35)

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived rhesus macaque IL-12 protein Rhesus Macaque IL-12 (p40) (Ile23 û Ser328) Accession # P48095 6-His tag Rhesus Macaque IL-12 (p35) (Arg57 û Ser253) Accession # NP_001038199 6-His tag N-terminus C-terminus

source short :

CHO

USD :

468 USD

alt names :

CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage