Recombinant Human VEGFR1/Flt-1 Fc Chimera Protein | R&D Systems 321-FL product information

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product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human VEGFR1/Flt-1 Fc Chimera Protein

catalog :

321-FL

quantity :

50 ug

price :

369 USD

citations: 36

Reference
Njau F, Shushakova N, Schenk H, Wulfmeyer V, Bollin R, Menne J, et al. Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice. PLoS ONE. 2020;15:e0218494 pubmed publisher
Verma M, Shimizu Motohashi Y, Asakura Y, Ennen J, Bosco J, Zhou Z, et al. Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy. PLoS Genet. 2019;15:e1008468 pubmed publisher
Buczek Thomas J, Rich C, Nugent M. Hypoxia Induced Heparan Sulfate Primes the Extracellular Matrix for Endothelial Cell Recruitment by Facilitating VEGF-Fibronectin Interactions. Int J Mol Sci. 2019;20: pubmed publisher
Verma M, Asakura Y, Murakonda B, Pengo T, Latroche C, Chazaud B, et al. Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling. Cell Stem Cell. 2018;23:530-543.e9 pubmed publisher
Wheeler K, Jena M, Pradhan B, Nayak N, Das S, Hsu C, et al. VEGF may contribute to macrophage recruitment and M2 polarization in the decidua. PLoS ONE. 2018;13:e0191040 pubmed publisher
Mamer S, Chen S, Weddell J, Palasz A, Wittenkeller A, Kumar M, et al. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics. Sci Rep. 2017;7:16439 pubmed publisher
Toivanen P, Nieminen T, Laakkonen J, Heikura T, Kaikkonen M, Yla Herttuala S. Snake venom VEGF Vammin induces a highly efficient angiogenic response in skeletal muscle via VEGFR-2/NRP specific signaling. Sci Rep. 2017;7:5525 pubmed publisher
Gibbens J, Morris R, Bowles T, Spencer S, Wallace K. Dysregulation of the Fas/FasL system in an experimental animal model of HELLP syndrome. Pregnancy Hypertens. 2017;8:26-30 pubmed publisher
LeBlanc M, Wang W, Chen X, Caberoy N, Guo F, Shen C, et al. Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy. J Exp Med. 2017;214:1029-1047 pubmed publisher
Yu Z, Mouillesseaux K, Kushner E, Bautch V. Tumor-Derived Factors and Reduced p53 Promote Endothelial Cell Centrosome Over-Duplication. PLoS ONE. 2016;11:e0168334 pubmed publisher
Malard B, Florens N, Lambert C, Juillard L. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study. Artif Organs. 2017;41:545-555 pubmed publisher
Kerr L, Donoghue J, Wilding A, Johns T. Axitinib Has Antiangiogenic and Antitumorigenic Activity in Myxoid Liposarcoma. Sarcoma. 2016;2016:3484673 pubmed
Han K, Chang J, Lee H, Azar D. Proangiogenic Interactions of Vascular Endothelial MMP14 With VEGF Receptor 1 in VEGFA-Mediated Corneal Angiogenesis. Invest Ophthalmol Vis Sci. 2016;57:3313-22 pubmed publisher
Teran M, Nugent M. Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity. J Biol Chem. 2015;290:16451-62 pubmed publisher
Yi K, Jung S, Cho G, Seol H, Hong S, Oh M, et al. Effects of sFlt-1 and alpha 2-macroglobulin on vascular endothelial growth factor-induced endothelin-1 upregulation in human microvascular endothelial cells. Placenta. 2014;35:64-9 pubmed publisher
Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, et al. Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype. FASEB J. 2014;28:692-704 pubmed publisher
Qi J, Ebrahem Q, Ali M, Cutler A, Bell B, Prayson N, et al. Tissue inhibitor of metalloproteinases-3 peptides inhibit angiogenesis and choroidal neovascularization in mice. PLoS ONE. 2013;8:e55667 pubmed publisher
Tabata T, Petitt M, Fang Hoover J, Rivera J, Nozawa N, Shiboski S, et al. Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model. Am J Pathol. 2012;181:1540-59 pubmed publisher
Schreurs M, Houston E, May V, Cipolla M. The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy. FASEB J. 2012;26:355-62 pubmed publisher
Franco M, Roswall P, Cortez E, Hanahan D, Pietras K. Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression. Blood. 2011;118:2906-17 pubmed publisher
Goyal A, Pal N, Concannon M, Paul M, Doran M, Poluzzi C, et al. Endorepellin, the angiostatic module of perlecan, interacts with both the ?2?1 integrin and vascular endothelial growth factor receptor 2 (VEGFR2): a dual receptor antagonism. J Biol Chem. 2011;286:25947-62 pubmed publisher
Nishiguchi K, Kataoka K, Kachi S, Komeima K, Terasaki H. Regulation of pathologic retinal angiogenesis in mice and inhibition of VEGF-VEGFR2 binding by soluble heparan sulfate. PLoS ONE. 2010;5:e13493 pubmed publisher
Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, et al. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment. FASEB J. 2010;24:3782-95 pubmed publisher
Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, et al. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J Immunol. 2009;183:8176-85 pubmed publisher
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Red Horse K, Rivera J, Schanz A, Zhou Y, Winn V, Kapidzic M, et al. Cytotrophoblast induction of arterial apoptosis and lymphangiogenesis in an in vivo model of human placentation. J Clin Invest. 2006;116:2643-52 pubmed
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product information

brand :

R&D Systems

master code :

321-FL

SKU :

321-FL-050

product name :

Recombinant Human VEGFR1/Flt-1 Fc Chimera Protein

description :

The Recombinant Human VEGFR1/Flt-1 Fc Chimera Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human VEGFR1/Flt-1 Fc Chimera Protein has been validated for the following applications: Bioactivity.

target :

VEGFR1/Flt-1

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in MOPS, NaCl and CHAPS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

123 kDa, reducing conditions

theoretical molecular weight :

100 kDa (monomer)

gene symbol :

FLT1

details of functionality :

Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. Conn, G. et al . (1990) Proc. Natl. Acad. Sci. USA 87 :1323. The ED50 for this effect is 5-30 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

321-FL

accessionNumbers :

AAC50060

applications :

Bioactivity

source :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived human VEGFR1/Flt-1 protein Human VEGFR1 (Ser27-His687) Accession # AAC50060 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag N-terminus C-terminus

2020 USD :

369

2021 USD :

369 USD

alt names :

EC 2.7.10, EC 2.7.10.1, FLT, FLT1, FLT-1, Fms-like tyrosine kinase 1, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor), FRT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, VEGF R1, VEGFR1, VEGFR-1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.