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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-17A Protein, CF
catalog :
317-ILB-050
quantity :
50 ug
price :
388 USD
more info or order :
citations: 87
Reference
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Ainscough J, MacLeod T, McGonagle D, Brakefield R, Baron J, Alase A, et al. Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36?. Proc Natl Acad Sci U S A. 2017;114:E2748-E2757 pubmed publisher
Wang B, Cao P, Wang Z, Li Z, Wang Z, Ma J, et al. Interferon-γ-induced insufficient autophagy contributes to p62-dependent apoptosis of epithelial cells in chronic rhinosinusitis with nasal polyps. Allergy. 2017;72:1384-1397 pubmed publisher
Hull D, Cooksley H, Chokshi S, Williams R, Abraham S, Taylor P. Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis. Arthritis Res Ther. 2016;18:303 pubmed publisher
Huang Q, Chu S, Yin X, Yu X, Kang C, Li X, et al. Interleukin-17A-Induced Epithelial-Mesenchymal Transition of Human Intrahepatic Biliary Epithelial Cells: Implications for Primary Biliary Cirrhosis. Tohoku J Exp Med. 2016;240:269-275 pubmed
Tsaousi A, Witte E, Witte K, Röwert Huber H, Volk H, Sterry W, et al. MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations. Mediators Inflamm. 2016;2016:4097574 pubmed
Haruta K, Otaki N, Nagamine M, Kayo T, Sasaki A, Hiramoto S, et al. A Novel PEGylation Method for Improving the Pharmacokinetic Properties of Anti-Interleukin-17A RNA Aptamers. Nucleic Acid Ther. 2017;27:36-44 pubmed publisher
Fujii S, Suzuki K, Kawamoto A, Ishibashi F, Nakata T, Murano T, et al. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells. Sci Rep. 2016;6:36795 pubmed publisher
Liu S, Desharnais J, Sahasrabudhe P, Jin P, Li W, Oates B, et al. Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide. Sci Rep. 2016;6:26071 pubmed publisher
Garcia Bates T, Kim E, Concha Benavente F, Trivedi S, Mailliard R, Gambotto A, et al. Enhanced Cytotoxic CD8 T Cell Priming Using Dendritic Cell-Expressing Human Papillomavirus-16 E6/E7-p16INK4 Fusion Protein with Sequenced Anti-Programmed Death-1. J Immunol. 2016;196:2870-8 pubmed publisher
Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, et al. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis. J Exp Med. 2015;212:619-31 pubmed publisher
Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska Mrowiecka M, et al. The expression and regulation of chemerin in the epidermis. PLoS ONE. 2015;10:e0117830 pubmed publisher
Adams K, Abraham V, Spielman D, Kolls J, Rubenstein R, Conner G, et al. IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells. PLoS ONE. 2014;9:e103263 pubmed publisher
Wu L, Wang C, Boisson B, Misra S, Rayman P, Finke J, et al. The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling. J Immunol. 2014;193:1590-9 pubmed publisher
Rabeony H, Petit Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, et al. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1?, TNF? and oncostatin M. PLoS ONE. 2014;9:e101937 pubmed publisher
Wang Y, Wu H, Wu X, Bian Z, Gao Q. Interleukin 17A promotes gastric cancer invasiveness via NF-?B mediated matrix metalloproteinases 2 and 9 expression. PLoS ONE. 2014;9:e96678 pubmed publisher
Xia W, Bai J, Wu X, Wei Y, Feng S, Li L, et al. Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway. PLoS ONE. 2014;9:e98915 pubmed publisher
Macleod A, Rudolph R, Corriden R, Ye I, Garijo O, Havran W. Skin-resident T cells sense ultraviolet radiation-induced injury and contribute to DNA repair. J Immunol. 2014;192:5695-702 pubmed publisher
Ardeljan D, Wang Y, Park S, Shen D, Chu X, Yu C, et al. Interleukin-17 retinotoxicity is prevented by gene transfer of a soluble interleukin-17 receptor acting as a cytokine blocker: implications for age-related macular degeneration. PLoS ONE. 2014;9:e95900 pubmed publisher
Chiricozzi A, Nograles K, Johnson Huang L, Fuentes Duculan J, Cardinale I, Bonifacio K, et al. IL-17 induces an expanded range of downstream genes in reconstituted human epidermis model. PLoS ONE. 2014;9:e90284 pubmed publisher
Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, et al. The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis. J Immunol. 2013;190:5256-66 pubmed publisher
Zizzo G, Cohen P. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids. J Immunol. 2013;190:5237-46 pubmed publisher
Rosen L, Freeman A, Yang L, Jutivorakool K, Olivier K, Angkasekwinai N, et al. Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis. J Immunol. 2013;190:3959-66 pubmed publisher
Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, et al. Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis. Hum Mol Genet. 2013;22:1807-15 pubmed publisher
Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, et al. Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement. Arthritis Rheum. 2013;65:1347-56 pubmed publisher
Gordon K, Kochkodan J, Blatt H, Lin S, Kaplan N, Johnston A, et al. Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis. J Invest Dermatol. 2013;133:712-722 pubmed publisher
Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, et al. IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation. PLoS Biol. 2012;10:e1001395 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Ramirez Carrozzi V, Sambandam A, Luis E, Lin Z, Jeet S, Lesch J, et al. IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Nat Immunol. 2011;12:1159-66 pubmed publisher
Liu Y, Mei J, Gonzales L, Yang G, Dai N, Wang P, et al. IL-17A and TNF-? exert synergistic effects on expression of CXCL5 by alveolar type II cells in vivo and in vitro. J Immunol. 2011;186:3197-205 pubmed publisher
Johnston A, Xing X, Guzman A, Riblett M, Loyd C, Ward N, et al. IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression. J Immunol. 2011;186:2613-22 pubmed publisher
Gudjonsson J, Johnston A, Stoll S, Riblett M, Xing X, Kochkodan J, et al. Evidence for altered Wnt signaling in psoriatic skin. J Invest Dermatol. 2010;130:1849-59 pubmed publisher
Shan M, Cheng H, Song L, Roberts L, Green L, Hacken Bitar J, et al. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med. 2009;1:4ra10 pubmed publisher
Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, et al. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. J Immunol. 2010;184:4880-8 pubmed publisher
Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, et al. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1{alpha}, and TNF-{alpha} Recapitulates Some Features of Psoriasis. J Immunol. 2010;184:5263-5270 pubmed publisher
Kisand K, Bøe Wolff A, Podkrajsek K, Tserel L, Link M, Kisand K, et al. Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines. J Exp Med. 2010;207:299-308 pubmed publisher
Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y. Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Gut. 2010;59:531-41 pubmed publisher
Xu G, Zhang L, Wang D, Xu R, Liu Z, Han D, et al. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy. 2010;65:581-9 pubmed publisher
Heo Y, Joo Y, Oh H, Park M, Heo Y, Cho M, et al. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol Lett. 2010;127:150-6 pubmed publisher
Wolk K, Witte E, Warszawska K, Schulze Tanzil G, Witte K, Philipp S, et al. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur J Immunol. 2009;39:3570-81 pubmed publisher
Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, et al. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur J Immunol. 2009;39:2779-88 pubmed publisher
Nishida A, Andoh A, Inatomi O, Fujiyama Y. Interleukin-32 expression in the pancreas. J Biol Chem. 2009;284:17868-76 pubmed publisher
Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, et al. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int J Cancer. 2009;125:367-73 pubmed publisher
Shahrara S, Pickens S, Dorfleutner A, Pope R. IL-17 induces monocyte migration in rheumatoid arthritis. J Immunol. 2009;182:3884-91 pubmed publisher
Amiable N, Tat S, Lajeunesse D, Duval N, Pelletier J, Martel Pelletier J, et al. Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Bone. 2009;44:1143-50 pubmed publisher
Eid R, Rao D, Zhou J, Lo S, Ranjbaran H, Gallo A, et al. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation. 2009;119:1424-32 pubmed publisher
Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis M, Miossec P. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. J Immunol. 2009;182:3112-20 pubmed publisher
Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, et al. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J Allergy Clin Immunol. 2009;123:59-66.e4 pubmed publisher
Kinoshita H, Takai T, Le T, Kamijo S, Wang X, Ushio H, et al. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol. 2009;123:179-86 pubmed publisher
Kwan Tat S, Pelletier J, Amiable N, Boileau C, Lajeunesse D, Duval N, et al. Activation of the receptor EphB4 by its specific ligand ephrin B2 in human osteoarthritic subchondral bone osteoblasts. Arthritis Rheum. 2008;58:3820-30 pubmed publisher
Guttman Yassky E, Lowes M, Fuentes Duculan J, Zaba L, Cardinale I, Nograles K, et al. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. J Immunol. 2008;181:7420-7 pubmed
Rahmoun M, Moles J, Pedretti N, Mathieu M, Fremaux I, Raison Peyron N, et al. Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils. J Invest Dermatol. 2009;129:671-81 pubmed publisher
Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, et al. Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis. J Invest Dermatol. 2009;129:650-6 pubmed publisher
Rickel E, Siegel L, Yoon B, Rottman J, Kugler D, Swart D, et al. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J Immunol. 2008;181:4299-310 pubmed
Wright J, Bennett F, Li B, Brooks J, Luxenberg D, Whitters M, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008;181:2799-805 pubmed
Kato A, Peters A, Suh L, Carter R, Harris K, Chandra R, et al. Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. J Allergy Clin Immunol. 2008;121:1385-92, 1392.e1-2 pubmed publisher
Aujla S, Chan Y, Zheng M, Fei M, Askew D, Pociask D, et al. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia. Nat Med. 2008;14:275-81 pubmed publisher
Zrioual S, Toh M, Tournadre A, Zhou Y, Cazalis M, Pachot A, et al. IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J Immunol. 2008;180:655-63 pubmed
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
Shioya M, Nishida A, Yagi Y, Ogawa A, Tsujikawa T, Kim Mitsuyama S, et al. Epithelial overexpression of interleukin-32alpha in inflammatory bowel disease. Clin Exp Immunol. 2007;149:480-6 pubmed
Wolk K, Witte E, Hoffmann U, Doecke W, Endesfelder S, Asadullah K, et al. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease. J Immunol. 2007;178:5973-81 pubmed
Brechter A, Lerner U. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression. Arthritis Rheum. 2007;56:910-23 pubmed
Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, et al. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Kidney Int. 2007;71:514-25 pubmed
Filer A, Parsonage G, Smith E, Osborne C, Thomas A, Curnow S, et al. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum. 2006;54:2096-108 pubmed
Vaknin Dembinsky A, Balashov K, Weiner H. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production. J Immunol. 2006;176:7768-74 pubmed
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, et al. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J Immunol. 2005;175:6177-89 pubmed
image
image 1 :
R&D Systems 317-ILB-050 image 1
Recombinant Human IL-17A Protein Bioactivity
product information
brand :
R&D Systems
master code :
317-ILB
SKU :
317-ILB-050
product name :
Recombinant Human IL-17A Protein, CF
unit size :
50 ug
description :
The Human Apo-Transferrin Protein, CF from R&D Systems is derived from Human Plasma. The Human Apo-Transferrin Protein, CF has been validated for the following applications: Bioactivity.
target :
IL-17/IL-17A
category :
Proteins and Enzymes
alt names :
CTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A
buffer :
Lyophilized from a 0.2 µm filtered solution in HCl.
conjugate :
Unconjugated
all available conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
theoretical molecular weight :
16 kDa (monomer)
gene symbol :
IL17A
details of functionality :
Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 0.4-4 ng/mL. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al . (1995) Immunity 3 :811. The ED50 for this effect is 1.5-7.5 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q16552
applications :
Bioactivity
source :
E. coli-derived Recombinant Human IL-17A Protein, CF
extended description :
New rh IL-17A Available! ~4 fold better activity; HEK293 expressed; Lower endotoxin specification!
USD :
359
Jul-22 :
388 USD
product details :
New rh IL-17A Available! ~4 fold better activity; HEK293 expressed; Lower endotoxin specification!
sizes available :
50 ug
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.