Recombinant Human TLR4/MD2 Complex Protein | R&D Systems 3146-TM product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human TLR4/MD2 Complex Protein

catalog :

3146-TM

quantity :

50 ug

price :

329 USD

citations: 15

Reference
Kasus Jacobi A, Land C, Stock A, Washburn J, Pereira H. Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9. Invest Ophthalmol Vis Sci. 2020;61:16 pubmed publisher
Vogl T, Stratis A, Wixler V, Voller T, Thurainayagam S, Jorch S, et al. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018;128:1852-1866 pubmed publisher
Sun S, He M, Wang Y, Yang H, Al Abed Y. Folic acid derived-P5779 mimetics regulate DAMP-mediated inflammation through disruption of HMGB1:TLR4:MD-2 axes. PLoS ONE. 2018;13:e0193028 pubmed publisher
Honegr J, Dolezal R, Malinak D, Benkova M, Soukup O, Almeida J, et al. Rational Design of a New Class of Toll-Like Receptor 4 (TLR4) Tryptamine Related Agonists by Means of the Structure- and Ligand-Based Virtual Screening for Vaccine Adjuvant Discovery. Molecules. 2018;23: pubmed publisher
Durai P, Shin H, Achek A, Kwon H, Govindaraj R, Panneerselvam S, et al. Toll-like receptor 2 antagonists identified through virtual screening and experimental validation. FEBS J. 2017;284:2264-2283 pubmed publisher
Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby Teglund A, Smeds E, et al. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog. 2015;11:e1005319 pubmed publisher
Loyau J, Malinge P, Daubeuf B, Shang L, Elson G, Kosco Vilbois M, et al. Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to FcÎ³ receptors. MAbs. 2014;6:1621-30 pubmed publisher
Jung I, Shin S, Lee M, Kang T, Han H, Lee S, et al. Enhancement of tumor-specific T cell-mediated immunity in dendritic cell-based vaccines by Mycobacterium tuberculosis heat shock protein X. J Immunol. 2014;193:1233-45 pubmed publisher
Shang L, Daubeuf B, Triantafilou M, Olden R, Dépis F, Raby A, et al. Selective antibody intervention of Toll-like receptor 4 activation through Fc ? receptor tethering. J Biol Chem. 2014;289:15309-18 pubmed publisher
Chen Y, Shiau A, Wang S, Yang J, Chang S, Wu C, et al. Zhankuic acid A isolated from Taiwanofungus camphoratus is a novel selective TLR4/MD-2 antagonist with anti-inflammatory properties. J Immunol. 2014;192:2778-86 pubmed publisher
Pal D, Dasgupta S, Kundu R, Maitra S, Das G, Mukhopadhyay S, et al. Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nat Med. 2012;18:1279-85 pubmed publisher
Feng C, Stamatos N, Dragan A, Medvedev A, Whitford M, Zhang L, et al. Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex. PLoS ONE. 2012;7:e32359 pubmed publisher
Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, et al. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding. PLoS ONE. 2011;6:e23132 pubmed publisher
Chambers M, Whelan A, Spallek R, Singh M, Coddeville B, Guerardel Y, et al. Non-acylated Mycobacterium bovis glycoprotein MPB83 binds to TLR1/2 and stimulates production of matrix metalloproteinase 9. Biochem Biophys Res Commun. 2010;400:403-8 pubmed publisher
Zunt S, Burton L, Goldblatt L, Dobbins E, Srinivasan M. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells. Clin Exp Immunol. 2009;156:285-93 pubmed publisher

product information

brand :

R&D Systems

master code :

3146-TM

SKU :

3146-TM-050

product name :

Recombinant Human TLR4/MD2 Complex Protein

description :

The Recombinant Human TLR4/MD2 Complex Protein from R&D Systems is derived from NS0. The Recombinant Human TLR4/MD2 Complex Protein has been validated for the following applications: Bioactivity.

target :

TLR4/MD-2 Complex

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

95 kDa and 34 kDa, reducing conditions

theoretical molecular weight :

70.6 kDa (TLR4), 19.2 kDa (MD-2)

gene symbol :

TLR4

details of functionality :

Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. 20 µg/mL of recombinant human TLR4/MD-2 will inhibit 60% of the TNF-alpha secretion induced by LPS.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

3146-TM

accessionNumbers :

O00206.2 (TLR4) Q9Y6Y9.1 (MD-2)

applications :

Bioactivity

source :

Mouse myeloma cell line, NS0-derived human TLR4/MD2 Complex protein Human TLR4 (Glu24-Lys631) Accession # O00206.2 S 10-His tag Human MD-2 (Glu17-Asn160) Accession # Q9Y6Y9.1 IEGRGGGSGGGSGGGS 10-His tag N-terminus C-terminus

2020 USD :

329

2021 USD :

329 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.