product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human TLR4/MD2 Complex Protein
catalog :
3146-TM-050
quantity :
50 ug
price :
377 USD
more info or order :
R&D Systems product webpage
citations: 20
Reference
Xu H, Chen G, Wu Y, Xie L, Tan Z, Liu B, et al. Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts anti-inflammatory effect by direct inhibiting toll like receptor 4 signaling pathway. J Ginseng Res. 2022;46:156-166 pubmed publisher
Tsubokawa D, Kikuchi T, Lee J, Kusakabe T, Yamamoto Y, Maruyama H. Venestatin from parasitic helminths interferes with receptor for advanced glycation end products (RAGE)-mediated immune responses to promote larval migration. PLoS Pathog. 2021;17:e1009649 pubmed publisher
Iannucci A, Caneparo V, Raviola S, Debernardi I, Colangelo D, Miggiano R, et al. Toll-like receptor 4-mediated inflammation triggered by extracellular IFI16 is enhanced by lipopolysaccharide binding. PLoS Pathog. 2020;16:e1008811 pubmed publisher
Jaeckle E, Brauchle E, Nottrodt N, Wehner M, Lensing R, Gillner A, et al. Towards automation in biologics production via Raman micro-spectroscopy, laser-induced forward cell transfer and surface-enhanced Raman spectroscopy. J Biotechnol. 2020;323:313-321 pubmed publisher
Bahraoui E, Serrero M, Planès R. HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway. Sci Rep. 2020;10:8177 pubmed publisher
Kasus Jacobi A, Land C, Stock A, Washburn J, Pereira H. Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9. Invest Ophthalmol Vis Sci. 2020;61:16 pubmed publisher
Vogl T, Stratis A, Wixler V, Voller T, Thurainayagam S, Jorch S, et al. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018;128:1852-1866 pubmed publisher
Sun S, He M, Wang Y, Yang H, Al Abed Y. Folic acid derived-P5779 mimetics regulate DAMP-mediated inflammation through disruption of HMGB1:TLR4:MD-2 axes. PLoS ONE. 2018;13:e0193028 pubmed publisher
Honegr J, Dolezal R, Malinak D, Benkova M, Soukup O, Almeida J, et al. Rational Design of a New Class of Toll-Like Receptor 4 (TLR4) Tryptamine Related Agonists by Means of the Structure- and Ligand-Based Virtual Screening for Vaccine Adjuvant Discovery. Molecules. 2018;23: pubmed publisher
Durai P, Shin H, Achek A, Kwon H, Govindaraj R, Panneerselvam S, et al. Toll-like receptor 2 antagonists identified through virtual screening and experimental validation. FEBS J. 2017;284:2264-2283 pubmed publisher
Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby Teglund A, Smeds E, et al. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog. 2015;11:e1005319 pubmed publisher
Loyau J, Malinge P, Daubeuf B, Shang L, Elson G, Kosco Vilbois M, et al. Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors. MAbs. 2014;6:1621-30 pubmed publisher
Jung I, Shin S, Lee M, Kang T, Han H, Lee S, et al. Enhancement of tumor-specific T cell-mediated immunity in dendritic cell-based vaccines by Mycobacterium tuberculosis heat shock protein X. J Immunol. 2014;193:1233-45 pubmed publisher
Shang L, Daubeuf B, Triantafilou M, Olden R, Dépis F, Raby A, et al. Selective antibody intervention of Toll-like receptor 4 activation through Fc ? receptor tethering. J Biol Chem. 2014;289:15309-18 pubmed publisher
Chen Y, Shiau A, Wang S, Yang J, Chang S, Wu C, et al. Zhankuic acid A isolated from Taiwanofungus camphoratus is a novel selective TLR4/MD-2 antagonist with anti-inflammatory properties. J Immunol. 2014;192:2778-86 pubmed publisher
Pal D, Dasgupta S, Kundu R, Maitra S, Das G, Mukhopadhyay S, et al. Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nat Med. 2012;18:1279-85 pubmed publisher
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product information
brand :
R&D Systems
master code :
3146-TM
SKU :
3146-TM-050
product name :
Recombinant Human TLR4/MD2 Complex Protein
description :
The Recombinant Human TLR4/MD2 Complex Protein from R&D Systems is derived from NS0. The Recombinant Human TLR4/MD2 Complex Protein has been validated for the following applications: Bioactivity.
target :
TLR4/MD2 Complex
category :
Proteins and Enzymes
sizes available :
50 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
95 kDa and 34 kDa, reducing conditions
theoretical molecular weight :
70.6 kDa (TLR4), 19.2 kDa (MD-2)
gene symbol :
TLR4
details of functionality :
Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. 20 µg/mL of recombinant human TLR4/MD-2 will inhibit 60% of the TNF-alpha secretion induced by LPS.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
O00206.2 (TLR4) Q9Y6Y9.1 (MD-2)
applications :
Bioactivity
source :
NS0-derived Recombinant Human TLR4/MD2 Complex Protein
USD :
377
USD 2023 :
377 USD
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.