Recombinant Human TLR4/MD-2 Complex Protein, CF | R&D Systems 3146-TM-050/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human TLR4/MD-2 Complex Protein, CF

catalog :

3146-TM-050/CF

quantity :

50 ug

price :

400 USD

citations: 21

Reference
Wang X, Mayorga Flores M, Bien K, Bailey A, Iwahara J. DNA-mediated proteolysis by neutrophil elastase enhances binding activities of the HMGB1 protein. J Biol Chem. 2022;298:102577 pubmed publisher
Xu H, Chen G, Wu Y, Xie L, Tan Z, Liu B, et al. Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts anti-inflammatory effect by direct inhibiting toll like receptor 4 signaling pathway. J Ginseng Res. 2022;46:156-166 pubmed publisher
Tsubokawa D, Kikuchi T, Lee J, Kusakabe T, Yamamoto Y, Maruyama H. Venestatin from parasitic helminths interferes with receptor for advanced glycation end products (RAGE)-mediated immune responses to promote larval migration. PLoS Pathog. 2021;17:e1009649 pubmed publisher
Iannucci A, Caneparo V, Raviola S, Debernardi I, Colangelo D, Miggiano R, et al. Toll-like receptor 4-mediated inflammation triggered by extracellular IFI16 is enhanced by lipopolysaccharide binding. PLoS Pathog. 2020;16:e1008811 pubmed publisher
Jaeckle E, Brauchle E, Nottrodt N, Wehner M, Lensing R, Gillner A, et al. Towards automation in biologics production via Raman micro-spectroscopy, laser-induced forward cell transfer and surface-enhanced Raman spectroscopy. J Biotechnol. 2020;323:313-321 pubmed publisher
Bahraoui E, Serrero M, Planès R. HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway. Sci Rep. 2020;10:8177 pubmed publisher
Kasus Jacobi A, Land C, Stock A, Washburn J, Pereira H. Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9. Invest Ophthalmol Vis Sci. 2020;61:16 pubmed publisher
Vogl T, Stratis A, Wixler V, Voller T, Thurainayagam S, Jorch S, et al. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018;128:1852-1866 pubmed publisher
Sun S, He M, Wang Y, Yang H, Al Abed Y. Folic acid derived-P5779 mimetics regulate DAMP-mediated inflammation through disruption of HMGB1:TLR4:MD-2 axes. PLoS ONE. 2018;13:e0193028 pubmed publisher
Honegr J, Dolezal R, Malinak D, Benkova M, Soukup O, Almeida J, et al. Rational Design of a New Class of Toll-Like Receptor 4 (TLR4) Tryptamine Related Agonists by Means of the Structure- and Ligand-Based Virtual Screening for Vaccine Adjuvant Discovery. Molecules. 2018;23: pubmed publisher
Durai P, Shin H, Achek A, Kwon H, Govindaraj R, Panneerselvam S, et al. Toll-like receptor 2 antagonists identified through virtual screening and experimental validation. FEBS J. 2017;284:2264-2283 pubmed publisher
Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby Teglund A, Smeds E, et al. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog. 2015;11:e1005319 pubmed publisher
Loyau J, Malinge P, Daubeuf B, Shang L, Elson G, Kosco Vilbois M, et al. Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to FcÎ³ receptors. MAbs. 2014;6:1621-30 pubmed publisher
Jung I, Shin S, Lee M, Kang T, Han H, Lee S, et al. Enhancement of tumor-specific T cell-mediated immunity in dendritic cell-based vaccines by Mycobacterium tuberculosis heat shock protein X. J Immunol. 2014;193:1233-45 pubmed publisher
Shang L, Daubeuf B, Triantafilou M, Olden R, Dépis F, Raby A, et al. Selective antibody intervention of Toll-like receptor 4 activation through Fc ? receptor tethering. J Biol Chem. 2014;289:15309-18 pubmed publisher
Chen Y, Shiau A, Wang S, Yang J, Chang S, Wu C, et al. Zhankuic acid A isolated from Taiwanofungus camphoratus is a novel selective TLR4/MD-2 antagonist with anti-inflammatory properties. J Immunol. 2014;192:2778-86 pubmed publisher
Pal D, Dasgupta S, Kundu R, Maitra S, Das G, Mukhopadhyay S, et al. Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nat Med. 2012;18:1279-85 pubmed publisher
Feng C, Stamatos N, Dragan A, Medvedev A, Whitford M, Zhang L, et al. Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex. PLoS ONE. 2012;7:e32359 pubmed publisher
Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, et al. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding. PLoS ONE. 2011;6:e23132 pubmed publisher
Chambers M, Whelan A, Spallek R, Singh M, Coddeville B, Guerardel Y, et al. Non-acylated Mycobacterium bovis glycoprotein MPB83 binds to TLR1/2 and stimulates production of matrix metalloproteinase 9. Biochem Biophys Res Commun. 2010;400:403-8 pubmed publisher
Zunt S, Burton L, Goldblatt L, Dobbins E, Srinivasan M. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells. Clin Exp Immunol. 2009;156:285-93 pubmed publisher

product information

master code :

3146-TM/CF

SKU :

3146-TM-050/CF

product name :

Recombinant Human TLR4/MD-2 Complex Protein, CF

unit size :

50 ug

description :

The Recombinant Human TLR4/MD-2 Complex Protein, CF from R&D Systems is derived from NS0. The Recombinant Human TLR4/MD-2 Complex Protein, CF has been validated for the following applications: Bioactivity.

target :

TLR4/MD2 Complex

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

95 kDa and 34 kDa, reducing conditions

theoretical molecular weight :

70.6 kDa (TLR4), 19.2 kDa (MD-2)

gene symbol :

TLR4

details of functionality :

Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. 20 ╡g/mL of recombinant human TLR4/MD-2 will inhibit 60% of the TNF-alpha secretion induced by LPS.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

O00206.2 (TLR4) Q9Y6Y9.1 (MD-2)

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human TLR4/MD2 Complex protein Human TLR4 (Glu24-Lys631) Accession # O00206.2 S 10-His tag Human MD-2 (Glu17-Asn160) Accession # Q9Y6Y9.1 IEGRGGGSGGGSGGGS 10-His tag N-terminus C-terminus

source short :

NS0

USD :

400 USD

alt names :

ARMD10;CD284;ESOP-1;lipopolysaccharide response;Ly-96;Lymphocyte antigen 96;myeloid differentiation factor-2;myeloid differentiation protein-2;Protein MD-2;TLR-4;TOLL;Toll-like receptor 4, CD284, ESOP-1, LY96, MD-2, TLR4/MD-2 Complex

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.