Recombinant Human Tie-2 Fc Chimera Protein, CF | R&D Systems 313-TI-100 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Tie-2 Fc Chimera Protein, CF

catalog :

313-TI-100

quantity :

100 ug

price :

410 USD

more info or order :

R&D Systems product webpage

citations: 15

Reference
Koya Y, Nara H, Yagi S, Ueno C, Kamohara M. An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1. Sci Rep. 2021;11:14021 pubmed publisher
Elamaa H, Kihlström M, Kapiainen E, Kaakinen M, Miinalainen I, Ragauskas S, et al. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina. elife. 2018;7: pubmed publisher
Thomson B, Souma T, Tompson S, Onay T, Kizhatil K, Siggs O, et al. Angiopoietin-1 is required for Schlemm's canal development in mice and humans. J Clin Invest. 2017;127:4421-4436 pubmed publisher
Shlamkovich T, Aharon L, Barton W, Papo N. Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy. Oncotarget. 2017;8:33571-33585 pubmed publisher
Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis E, et al. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells. PLoS ONE. 2016;11:e0156994 pubmed publisher
Yao J, Cui M, Li M, Liu Y, He Q, Xiao J, et al. Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis. PLoS ONE. 2014;9:e89148 pubmed publisher
Buchanan A, Clementel V, Woods R, Harn N, Bowen M, Mo W, et al. Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression. MAbs. 2013;5:255-62 pubmed publisher
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, et al. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat Cell Biol. 2008;10:527-37 pubmed publisher
Masckauchan T, Agalliu D, Vorontchikhina M, Ahn A, Parmalee N, Li C, et al. Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol Biol Cell. 2006;17:5163-72 pubmed
Red Horse K, Rivera J, Schanz A, Zhou Y, Winn V, Kapidzic M, et al. Cytotrophoblast induction of arterial apoptosis and lymphangiogenesis in an in vivo model of human placentation. J Clin Invest. 2006;116:2643-52 pubmed
Zhu T, Sennlaub F, Beauchamp M, Fan L, Joyal J, Checchin D, et al. Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent. Arterioscler Thromb Vasc Biol. 2006;26:744-50 pubmed
Popkov M, Jendreyko N, McGavern D, Rader C, Barbas C. Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody. Cancer Res. 2005;65:972-81 pubmed
Oliner J, Min H, Leal J, Yu D, Rao S, You E, et al. Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2. Cancer Cell. 2004;6:507-16 pubmed
Satchell S, Anderson K, Mathieson P. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties. J Am Soc Nephrol. 2004;15:566-74 pubmed
Caine G, Blann A, Stonelake P, Ryan P, Lip G. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur J Clin Invest. 2003;33:883-90 pubmed

image

image 1 :

Recombinant Human Tie-2 Fc Chimera Protein Binding Activity

product information

brand :

R&D Systems

master code :

313-TI

SKU :

313-TI-100

product name :

Recombinant Human Tie-2 Fc Chimera Protein, CF

description :

The Recombinant Human Tie-2 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Tie-2 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

Tie-2

category :

Proteins and Enzymes

sizes available :

100 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

135 kDa, reducing conditions

theoretical molecular weight :

100 kDa (monomer)

gene symbol :

TEK

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN ) binds with an ED50 of 0.5-4 ng/mL.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

Q02763

applications :

Bioactivity

source :

NS0-derived Recombinant Human Tie-2 Fc Chimera Protein, CF

USD :

410

USD 2023 :

410 USD

alt names :

angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK, TEK tyrosine kinase, endothelial, Tie2, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage