Recombinant Mouse Dkk-4 Protein | R&D Systems 3105-DK-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Dkk-4 Protein

catalog :

3105-DK-010

quantity :

10 ug

price :

541 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Fliniaux I, Mikkola M, Lefebvre S, Thesleff I. Identification of dkk4 as a target of Eda-A1/Edar pathway reveals an unexpected role of ectodysplasin as inhibitor of Wnt signalling in ectodermal placodes. Dev Biol. 2008;320:60-71 pubmed publisher

product information

master code :

3105-DK

SKU :

3105-DK-010

product name :

Recombinant Mouse Dkk-4 Protein

unit size :

10 ug

description :

The Recombinant Mouse Dkk-4 Protein from R&D Systems is derived from NS0. The Recombinant Mouse Dkk-4 Protein has been validated for the following applications: Bioactivity.

target :

Dkk-4

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Mouse

observed molecular weight :

31 kDa, reducing conditions

theoretical molecular weight :

23 kDa

gene symbol :

Dkk4

details of functionality :

Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 1-4 ╡g/mL. Optimal concentrations should be determined by each laboratory for each application. Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-4 (Catalog # 3105-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). The ED50 for this effect is 0.2-1.2 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Mouse Dkk-4 Protein Bioactivity

accessionNumbers :

Q8VEJ3

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived mouse Dkk-4 protein Leu19-Ile221 (Arg67Ser and Arg70Ser), with a C-terminal 6-His tag

source short :

NS0

USD :

541 USD

alt names :

dickkopf (Xenopus laevis) homolog 4, dickkopf homolog 4 (Xenopus laevis), dickkopf-4, dickkopf-related protein 4, Dkk4, DKK-4, hDkk-4, MGC129562, MGC129563

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage