Bacterial Thermolysin Protein, CF | R&D Systems 3097-ZN-020 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Bacterial Thermolysin Protein, CF

catalog :

3097-ZN-020

quantity :

20 ug

price :

143 USD

more info or order :

R&D Systems product webpage

citations: 9

Reference
Hao Y, Purtha W, Cortesio C, Rui H, Gu Y, Chen H, et al. Crystal structures of human procathepsin H. PLoS ONE. 2018;13:e0200374 pubmed publisher
Hofherr A, Wagner C, Fedeles S, Somlo S, Köttgen M. N-glycosylation determines the abundance of the transient receptor potential channel TRPP2. J Biol Chem. 2014;289:14854-67 pubmed publisher
Fortugno P, Bresciani A, Paolini C, Pazzagli C, El Hachem M, D Alessio M, et al. Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis. J Invest Dermatol. 2011;131:2223-32 pubmed publisher
Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder J, et al. Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptides. 2011;32:1187-92 pubmed publisher
Fernando S, Fletcher B. Targeting tumor endothelial marker 8 in the tumor vasculature of colorectal carcinomas in mice. Cancer Res. 2009;69:5126-32 pubmed publisher
Lilla J, Joshi R, Craik C, Werb Z. Active plasma kallikrein localizes to mast cells and regulates epithelial cell apoptosis, adipocyte differentiation, and stromal remodeling during mammary gland involution. J Biol Chem. 2009;284:13792-803 pubmed publisher
Ramani V, Haun R. The extracellular matrix protein fibronectin is a substrate for kallikrein 7. Biochem Biophys Res Commun. 2008;369:1169-73 pubmed publisher
Mukai S, Fukushima T, Naka D, Tanaka H, Osada Y, Kataoka H. Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases. FEBS J. 2008;275:1003-17 pubmed publisher
Gómez Gaviro M, Domínguez Luis M, Canchado J, Calafat J, Janssen H, Lara Pezzi E, et al. Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding. J Immunol. 2007;178:8053-63 pubmed

product information

brand :

R&D Systems

master code :

3097-ZN

SKU :

3097-ZN-020

product name :

Bacterial Thermolysin Protein, CF

description :

The Bacterial Thermolysin Protein, CF from R&D Systems is derived from G. stearothermophilus. The Bacterial Thermolysin Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Thermolysin

category :

Proteins and Enzymes

sizes available :

20 ug

buffer :

Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.

conjugate :

Unconjugated

purity :

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Bacterial

observed molecular weight :

36 kDa, reducing conditions

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005 ). The specific activity is 12,000 pmol/min/µg, as measured with 10 µM ES005 and 2 ng enzyme in 100 µL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

applications :

Enzyme Activity

source :

G. stearothermophilus

USD :

140

USD 2023 :

143 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

more info or order :

R&D Systems product webpage