Recombinant Human Flt-3 Ligand/FLT3L Protein | R&D Systems 308-FKN-005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Flt-3 Ligand/FLT3L Protein

catalog :

308-FKN-005

quantity :

5 ug (also 25 ug, 100 ug, 250 ug, 1 mg)

price :

191 USD

citations: 12

Reference
Kent G, Atkins M, Lung B, Nikitina A, Fernandes I, Kwan J, et al. Human liver sinusoidal endothelial cells support the development of functional human pluripotent stem cell-derived Kupffer cells. Cell Rep. 2024;43:114629 pubmed publisher
Bodansky A, Mettelman R, Sabatino J, Vazquez S, Chou J, Novak T, et al. Molecular mimicry in multisystem inflammatory syndrome in children. Nature. 2024;632:622-629 pubmed publisher
Smith A, Bowers S, Stratman A, Davis G. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. PLoS ONE. 2013;8:e85147 pubmed publisher
Ichii M, Frank M, Iozzo R, Kincade P. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood. 2012;119:1683-92 pubmed publisher
Uchida N, Hsieh M, Hayakawa J, Madison C, Washington K, Tisdale J. Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther. 2011;18:1078-86 pubmed publisher
Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, et al. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome). Blood. 2011;117:839-47 pubmed publisher
Trowbridge J, Guezguez B, Moon R, Bhatia M. Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells. 2010;28:1379-89 pubmed publisher
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, et al. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp Hematol. 2008;36:1121-31 pubmed publisher
McCullar V, Oostendorp R, Panoskaltsis Mortari A, Yun G, Lutz C, Wagner J, et al. Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15. Exp Hematol. 2008;36:598-608 pubmed publisher
Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, et al. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells. Cell Death Differ. 2006;13:1686-96 pubmed
Kersten C, Dosen G, Myklebust J, Sivertsen E, Hystad M, Smeland E, et al. BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3. Exp Hematol. 2006;34:72-81 pubmed
Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed

product information

master code :

308-FKN

SKU :

308-FKN-005

product name :

Recombinant Human Flt-3 Ligand/FLT3L Protein

unit size :

5 ug (also 25 ug, 100 ug, 250 ug, 1 mg)

description :

The Recombinant Human Flt-3 Ligand/FLT3L Protein from R&D Systems is derived from NS0. The Recombinant Human Flt-3 Ligand/FLT3L Protein has been validated for the following applications: Bioactivity.

target :

Flt-3 Ligand/FLT3L

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

Multiple bands between 24 kDa and 28 kDa, reducing conditions

theoretical molecular weight :

18 kDa

gene symbol :

FLT3LG

details of functionality :

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human Flt-3 Ligand/FLT3L Protein Bioactivity

accessionNumbers :

AAA17999

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Flt-3 Ligand/FLT3L protein Thr27-Pro185

source short :

NS0

USD :

191 USD

alt names :

FL, FLG3L, Flt3 ligand, Flt3L, FLT3LG, fms-related tyrosine kinase 3 ligand, SL cytokine

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.