Recombinant Human VEGF 165b Protein | R&D Systems 3045-VE-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human VEGF 165b Protein

catalog :

3045-VE-025

quantity :

25 ug

price :

420 USD

more info or order :

R&D Systems product webpage

citations: 6

Reference
Bucher F, Aguilar E, Marra K, Rapp J, Arnold J, Diaz Aguilar S, et al. CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10. Invest Ophthalmol Vis Sci. 2020;61:20 pubmed publisher
Boudria A, Abou Faycal C, Jia T, Gout S, Keramidas M, Didier C, et al. VEGF165b, a splice variant of VEGF-A, promotes lung tumor progression and escape from anti-angiogenic therapies through a β1 integrin/VEGFR autocrine loop. Oncogene. 2019;38:1050-1066 pubmed publisher
Jahangiri A, Nguyen A, Chandra A, Sidorov M, Yagnik G, Rick J, et al. Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer. Proc Natl Acad Sci U S A. 2017;114:E8685-E8694 pubmed publisher
Deissler H, Lang G, Lang G. Inhibition of Single Routes of Intracellular Signaling is Not Sufficient to Neutralize the Biphasic Disturbance of a Retinal Endothelial Cell Barrier Induced by VEGF-A165. Cell Physiol Biochem. 2017;42:1493-1513 pubmed publisher
Hulse R, Drake R, Bates D, Donaldson L. The control of alternative splicing by SRSF1 in myelinated afferents contributes to the development of neuropathic pain. Neurobiol Dis. 2016;96:186-200 pubmed publisher
Bates D, Mavrou A, Qiu Y, Carter J, Hamdollah Zadeh M, Barratt S, et al. Detection of VEGF-A(xxx)b isoforms in human tissues. PLoS ONE. 2013;8:e68399 pubmed publisher

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

3045-VE

SKU :

3045-VE-025

product name :

Recombinant Human VEGF 165b Protein

Description :

The Recombinant Human VEGF 165b Protein from R&D Systems is derived from Sf 21 (stably transfected). The Recombinant Human VEGF 165b Protein has been validated for the following applications: Bioactivity.

target :

VEGF

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

19-24 kDa, reducing conditions

theoretical molecular weight :

19.1 kDa (monomer)

gene symbol :

VEGFA

details of functionality :

Measured by its binding ability in a functional ELISA. Serially diluted Recombinant Human (rh) VEGF165b is added to a Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD ) coated plate (2 µg/mL, 100 µL/well). Bound rhVEGF165b is detected by a human VEGF165b specific antibody (Catalog # MAB3045 ). The concentration of rhVEGF165b that produces half-maximum response is approximately 0.12-0.6 nM.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

AAL27435

applications :

Bioactivity

source :

Sf 21 (stably transfected)-derived Recombinant Human VEGF 165b Protein

USD :

420 USD

alt names :

MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage