Recombinant Human VEGF 165b Protein, CF | R&D Systems 3045-VE-025/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human VEGF 165b Protein, CF

catalog :

3045-VE-025/CF

quantity :

25 ug

price :

425 USD

citations: 6

Reference
Bucher F, Aguilar E, Marra K, Rapp J, Arnold J, Diaz Aguilar S, et al. CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10. Invest Ophthalmol Vis Sci. 2020;61:20 pubmed publisher
Boudria A, Abou Faycal C, Jia T, Gout S, Keramidas M, Didier C, et al. VEGF165b, a splice variant of VEGF-A, promotes lung tumor progression and escape from anti-angiogenic therapies through a β1 integrin/VEGFR autocrine loop. Oncogene. 2019;38:1050-1066 pubmed publisher
Jahangiri A, Nguyen A, Chandra A, Sidorov M, Yagnik G, Rick J, et al. Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer. Proc Natl Acad Sci U S A. 2017;114:E8685-E8694 pubmed publisher
Deissler H, Lang G, Lang G. Inhibition of Single Routes of Intracellular Signaling is Not Sufficient to Neutralize the Biphasic Disturbance of a Retinal Endothelial Cell Barrier Induced by VEGF-A165. Cell Physiol Biochem. 2017;42:1493-1513 pubmed publisher
Hulse R, Drake R, Bates D, Donaldson L. The control of alternative splicing by SRSF1 in myelinated afferents contributes to the development of neuropathic pain. Neurobiol Dis. 2016;96:186-200 pubmed publisher
Bates D, Mavrou A, Qiu Y, Carter J, Hamdollah Zadeh M, Barratt S, et al. Detection of VEGF-A(xxx)b isoforms in human tissues. PLoS ONE. 2013;8:e68399 pubmed publisher

product information

master code :

3045-VE/CF

SKU :

3045-VE-025/CF

product name :

Recombinant Human VEGF 165b Protein, CF

unit size :

25 ug

description :

The Recombinant Human VEGF 165b Protein, CF from R&D Systems is derived from Sf 21 (stably transfected). The Recombinant Human VEGF 165b Protein, CF has been validated for the following applications: Bioactivity.

target :

VEGF

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

19-24 kDa, reducing conditions

theoretical molecular weight :

19.1 kDa (monomer)

gene symbol :

VEGFA

details of functionality :

Measured by its binding ability in a functional ELISA. Serially diluted Recombinant Human (rh) VEGF165b is added to a Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD ) coated plate (2 ╡g/mL, 100 ╡L/well). Bound rhVEGF165b is detected by a human VEGF165b specific antibody (Catalog # MAB3045 ). The concentration of rhVEGF165b that produces half-maximum response is approximately 0.12-0.6 nM.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

AAL27435

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (stably transfected)-derived human VEGF protein Ala27-Asp191

source short :

Sf 21 (stably transfected)

USD :

425 USD

alt names :

MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.