Recombinant Mouse Wnt-5b Protein | R&D Systems 3006-WN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Wnt-5b Protein

catalog :

3006-WN

quantity :

25 ug

price :

459 USD

citations: 4

Reference
Wu X, Van Dijk E, Ng Blichfeldt J, Bos I, Ciminieri C, Konigshoff M, et al. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors. Cells. 2019;8: pubmed publisher
Kessenbrock K, Smith P, Steenbeek S, Pervolarakis N, Kumar R, Minami Y, et al. Diverse regulation of mammary epithelial growth and branching morphogenesis through noncanonical Wnt signaling. Proc Natl Acad Sci U S A. 2017;114:3121-3126 pubmed publisher
Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol. 2014;385:291-303 pubmed publisher
Bourhis E, Tam C, Franke Y, Bazan J, Ernst J, Hwang J, et al. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010;285:9172-9 pubmed publisher

product information

brand :

R&D Systems

master code :

3006-WN

SKU :

3006-WN-025

product name :

Recombinant Mouse Wnt-5b Protein

description :

The Recombinant Mouse Wnt-5b Protein from R&D Systems is derived from CHO. The Recombinant Mouse Wnt-5b Protein has been validated for the following applications: Bioactivity.

target :

Wnt-5b

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>75%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

observed molecular weight :

45 kDa, reducing conditions

theoretical molecular weight :

38.6 kDa

details of functionality :

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

catalog number base :

3006-WN

accessionNumbers :

AAH10775

applications :

Bioactivity

source :

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-5b protein Gln31-Lys372

2020 USD :

459

2021 USD :

459 USD

alt names :

MGC2648, protein Wnt-5b, wingless-type MMTV integration site family, member 5B, Wnt5b, WNT-5B protein

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.