Recombinant Human Serpin E2/PN1 Protein, CF | R&D Systems 2980-PI-010 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Serpin E2/PN1 Protein, CF

catalog :

2980-PI-010

quantity :

10 ug

price :

432 USD

citations: 8

Reference
Szabo R, Bugge T. Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy. PLoS ONE. 2018;13:e0194660 pubmed publisher
Langer E, Kendsersky N, Daniel C, Kuziel G, Pelz C, Murphy K, et al. ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells. Oncogene. 2018;37:1005-1019 pubmed publisher
Wu X, Liu W, Duan Z, Gao Y, Li S, Wang K, et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep. 2016;6:30563 pubmed publisher
Santoro A, Conde J, Scotece M, Abella V, Lois A, Lopez V, et al. SERPINE2 Inhibits IL-1Î±-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-ÎºB/AP-1 Pathways. PLoS ONE. 2015;10:e0135979 pubmed publisher
Szabo R, Uzzun Sales K, Kosa P, Shylo N, Godiksen S, Hansen K, et al. Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. PLoS Genet. 2012;8:e1002937 pubmed publisher
Sales K, Masedunskas A, Bey A, Rasmussen A, Weigert R, List K, et al. Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome. Nat Genet. 2010;42:676-83 pubmed publisher
Myerburg M, McKenna E, Luke C, Frizzell R, Kleyman T, Pilewski J. Prostasin expression is regulated by airway surface liquid volume and is increased in cystic fibrosis. Am J Physiol Lung Cell Mol Physiol. 2008;294:L932-41 pubmed publisher
List K, Currie B, Scharschmidt T, Szabo R, Shireman J, Molinolo A, et al. Autosomal ichthyosis with hypotrichosis syndrome displays low matriptase proteolytic activity and is phenocopied in ST14 hypomorphic mice. J Biol Chem. 2007;282:36714-23 pubmed

product information

master code :

2980-PI

SKU :

2980-PI-010

product name :

Recombinant Human Serpin E2/PN1 Protein, CF

unit size :

10 ug

description :

The Recombinant Human Serpin E2/PN1 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Serpin E2/PN1 Protein, CF has been validated for the following applications: Inhibition Activity.

target :

Serpin E2/PN1

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Sodium Acetate, NaCl and Glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

50 kDa, reducing conditions

theoretical molecular weight :

43 kDa

gene symbol :

SERPINE2

details of functionality :

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The IC50 value is 2 nM, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_001130000

applications :

Inhibition Activity

source long :

Mouse myeloma cell line, NS0-derived human Serpin E2/PN1 protein Met1-Pro397, with a C-terminal 10-His tag

source short :

NS0

USD :

432 USD

alt names :

GDN, GdNPF, GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2, glia-derived nexin, glial-derived neurite promoting factor, Peptidase inhibitor 7, PI-7, PI7DKFZp686A13110, PN-1, PN1nexin, PNI, Protease nexin 1, Protease nexin I, Proteinase Nexin 1, Serpin E2, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.