Recombinant Human HGF (NS0-expressed) Protein, CF | R&D Systems 294-HGN-005/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human HGF (NS0-expressed) Protein, CF

catalog :

294-HGN-005/CF

quantity :

5 ug (also 25 ug, 100 ug, 250 ug)

price :

261 USD

more info or order :

R&D Systems product webpage

citations: 13

Reference
Gallo S, Vitacolonna A, Comoglio P, Crepaldi T. MET Oncogene Controls Invasive Growth by Coupling with NMDA Receptor. Cancers (Basel). 2022;14: pubmed publisher
Shimamura N, Fujii K, Ohkoshi S. Detection and quantification of human-specific mRNA from hepatocyte-like cells derived from dental pulp using real-time polymerase chain reaction. J Oral Biosci. 2021;63:298-305 pubmed publisher
Adame García S, Cervantes Villagrana R, Orduña Castillo L, Del Rio J, Gutkind J, Reyes Cruz G, et al. cAMP-dependent activation of the Rac guanine exchange factor P-REX1 by type I protein kinase A (PKA) regulatory subunits. J Biol Chem. 2019;294:2232-2246 pubmed publisher
Jahangiri A, Nguyen A, Chandra A, Sidorov M, Yagnik G, Rick J, et al. Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer. Proc Natl Acad Sci U S A. 2017;114:E8685-E8694 pubmed publisher
Narmada B, Goh Y, Li H, Sinha S, Yu H, Cheung C. Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals. Stem Cells Transl Med. 2017;6:851-863 pubmed publisher
He H, Kuriyan A, Su C, Mahabole M, Zhang Y, Zhu Y, et al. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3. Sci Rep. 2017;7:43736 pubmed publisher
Wang J, Goetsch L, Tucker L, Zhang Q, Gonzalez A, Vaidya K, et al. Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification. BMC Cancer. 2016;16:105 pubmed publisher
Tervonen T, BelitÅ¡kin D, Pant S, Englund J, Marques E, Ala Hongisto H, et al. Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion. Oncogene. 2016;35:1832-46 pubmed publisher
Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, et al. Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET. Clin Cancer Res. 2013;19:2381-92 pubmed publisher
Roelandt P, Pauwelyn K, Sancho Bru P, Subramanian K, Bose B, Ordovas L, et al. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE. 2010;5:e12101 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Jin M, Chen Y, He S, Ryan S, Hinton D. Hepatocyte growth factor and its role in the pathogenesis of retinal detachment. Invest Ophthalmol Vis Sci. 2004;45:323-9 pubmed
Barbour A, Reeder J, Walsh M, Fawcett J, Antalis T, Gotley D. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3. Cancer Res. 2003;63:887-92 pubmed

image

image 1 :

Recombinant Human HGF (NS0-expressed) Protein Bioactivity

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

294-HGN/CF

SKU :

294-HGN-005/CF

product name :

Recombinant Human HGF (NS0-expressed) Protein, CF

Description :

The Recombinant Human HGF (NS0-expressed) Protein, CF from R&D Systems is derived from NS0. The Recombinant Human HGF (NS0-expressed) Protein, CF has been validated for the following applications: Binding Activity.

target :

HGF

category :

Proteins and Enzymes

unit size :

5 ug (also 25 ug, 100 ug, 250 ug)

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

71-85 kDa, nonreducing conditions 56-65 kDa and 30-40 kDa, reducing conditions

theoretical molecular weight :

alpha chain: 53.7 kDa beta chain: 26 kDa

gene symbol :

HGF

details of functionality :

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al . (1999) Blood 94 :3883. The ED50 for this effect is equal to or less than 4.00 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P14210.2

applications :

Binding Activity

source :

NS0-derived Recombinant Human HGF (NS0-expressed) Protein

USD :

261 USD

alt names :

deafness, autosomal recessive 39, DFNB39, EC 3.4.21, EC 3.4.21.7, fibroblast-derived tumor cytotoxic factor, F-TCF, hepatocyte growth factor (hepapoietin A; scatter factor), Hepatopoeitin-A, Hepatopoietin A, HGFB, HPTA, HPTAhepatocyte growth factor, lung fibroblast-derived mitogen, Scatter factor, SF, SFhepatopoeitin-A

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage