product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human HGF Protein, CF
catalog :
294-HG-005/CF
quantity :
5 ug (also 25 ug, 100 ug)
price :
362 USD
more info or order :
citations: 98
Reference
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Kim Y, Lee J, An J, Kim J, Choi Y, Li L, et al. Renoprotective effects of a novel cMet agonistic antibody on kidney fibrosis. Sci Rep. 2019;9:13495 pubmed publisher
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Yu G, Wang Z, Zeng S, Liu S, Zhu C, Xu R, et al. Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma. Biomed Res Int. 2019;2019:9053295 pubmed publisher
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Okamoto R, Takayama K, Akita N, Nagamoto Y, Hosokawa D, Iizuka S, et al. Human iPS Cell-based Liver-like Tissue Engineering at Extrahepatic Sites in Mice as a New Cell Therapy for Hemophilia B. Cell Transplant. 2018;27:299-309 pubmed publisher
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Fagg W, Liu N, Yang M, Cheng K, Chung E, Kim J, et al. Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver. Cell Transplant. 2017;26:1868-1877 pubmed publisher
Yamane K, Mazaki T, Shiozaki Y, Yoshida A, Shinohara K, Nakamura M, et al. Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury. Sci Rep. 2018;8:917 pubmed publisher
Kanteti R, Mirzapoiazova T, Riehm J, Dhanasingh I, Mambetsariev B, Wang J, et al. Focal adhesion kinase a potential therapeutic target for pancreatic cancer and malignant pleural mesothelioma. Cancer Biol Ther. 2018;19:316-327 pubmed publisher
Han Y, Kang Y, Shivakumar S, Bharti D, Son Y, Choi Y, et al. Stem Cells from Cryopreserved Human Dental Pulp Tissues Sequentially Differentiate into Definitive Endoderm and Hepatocyte-Like Cells in vitro. Int J Med Sci. 2017;14:1418-1429 pubmed publisher
Owusu B, Thomas S, Venukadasula P, Han Z, Janetka J, Galemmo R, et al. Targeting the tumor-promoting microenvironment in MET-amplified NSCLC cells with a novel inhibitor of pro-HGF activation. Oncotarget. 2017;8:63014-63025 pubmed publisher
Park H, Kim D, Kim E, Sa J, Lee H, Yu S, et al. Tumor Inhibitory Effect of IRCR201, a Novel Cross-Reactive c-Met Antibody Targeting the PSI Domain. Int J Mol Sci. 2017;18: pubmed publisher
Clémenson C, Chargari C, Liu W, Mondini M, Ferte C, Burbridge M, et al. The MET/AXL/FGFR Inhibitor S49076 Impairs Aurora B Activity and Improves the Antitumor Efficacy of Radiotherapy. Mol Cancer Ther. 2017;16:2107-2119 pubmed publisher
Camp J, Sekine K, Gerber T, Loeffler Wirth H, Binder H, Gac M, et al. Multilineage communication regulates human liver bud development from pluripotency. Nature. 2017;546:533-538 pubmed publisher
Zou C, Lu Y, Teng X, Wang S, Sun X, Huang F, et al. MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys. Sci Rep. 2017;7:2505 pubmed publisher
Isshiki H, Arimura Y, Nagaishi K, Kawakami K, Onodera K, Yamashita K, et al. Establishment of a refined culture method for rat colon organoids. Biochem Biophys Res Commun. 2017;489:305-311 pubmed publisher
Mitani S, Takayama K, Nagamoto Y, Imagawa K, Sakurai F, Tachibana M, et al. Human ESC/iPSC-Derived Hepatocyte-like Cells Achieve Zone-Specific Hepatic Properties by Modulation of WNT Signaling. Mol Ther. 2017;25:1420-1433 pubmed publisher
Sakurai F, Mitani S, Yamamoto T, Takayama K, Tachibana M, Watashi K, et al. Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection. Sci Rep. 2017;7:45698 pubmed publisher
Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, et al. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes. Am J Hum Genet. 2017;100:617-634 pubmed publisher
Elberry D, Amin S, Esmail R, Rashed L, Gamal M. Effect of undifferentiated versus hepatogenic partially differentiated mesenchymal stem cells on hepatic and cognitive functions in liver cirrhosis. EXCLI J. 2016;15:652-670 pubmed publisher
Song Y, Jang J, Shin T, Bae S, Kim J, Kim K, et al. Sulfasalazine attenuates evading anticancer response of CD133-positive hepatocellular carcinoma cells. J Exp Clin Cancer Res. 2017;36:38 pubmed publisher
Weeden C, Chen Y, Ma S, Hu Y, Ramm G, Sutherland K, et al. Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway. PLoS Biol. 2017;15:e2000731 pubmed publisher
O Neill H, O Sullivan J, Porteous N, Ruiz Hernández E, Kelly H, O Brien F, et al. A collagen cardiac patch incorporating alginate microparticles permits the controlled release of hepatocyte growth factor and insulin-like growth factor-1 to enhance cardiac stem cell migration and proliferation. J Tissue Eng Regen Med. 2018;12:e384-e394 pubmed publisher
Lee S, Chong J, Min S, Bak S, Kim K. Are Adipose-Derived Stem Cells From Liver Falciform Ligaments Another Possible Source of Mesenchymal Stem Cells?. Cell Transplant. 2017;26:855-866 pubmed publisher
Chang H, Sung J, Moon S, Kim H, Kim J, Lee J. EGF Induced RET Inhibitor Resistance in CCDC6-RET Lung Cancer Cells. Yonsei Med J. 2017;58:9-18 pubmed publisher
Wang J, Anderson M, Oleksijew A, Vaidya K, Boghaert E, Tucker L, et al. ABBV-399, a c-Met Antibody-Drug Conjugate that Targets Both MET-Amplified and c-Met-Overexpressing Tumors, Irrespective of MET Pathway Dependence. Clin Cancer Res. 2017;23:992-1000 pubmed publisher
El Khatib M, Ohmine S, Jacobus E, Tonne J, Morsy S, Holditch S, et al. Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration. Stem Cells Transl Med. 2016;5:694-702 pubmed publisher
Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, et al. High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFκB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts. PLoS ONE. 2015;10:e0124283 pubmed publisher
Klijn C, Durinck S, Stawiski E, Haverty P, Jiang Z, Liu H, et al. A comprehensive transcriptional portrait of human cancer cell lines. Nat Biotechnol. 2015;33:306-12 pubmed publisher
Falcon B, Swearingen M, Gough W, Lee L, Foreman R, Uhlik M, et al. An in vitro cord formation assay identifies unique vascular phenotypes associated with angiogenic growth factors. PLoS ONE. 2014;9:e106901 pubmed publisher
Korhan P, Erdal E, Kandemiş E, Cokakli M, Nart D, Yilmaz F, et al. Reciprocal activating crosstalk between c-Met and caveolin 1 promotes invasive phenotype in hepatocellular carcinoma. PLoS ONE. 2014;9:e105278 pubmed publisher
Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, et al. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis. J Biol Chem. 2014;289:22835-49 pubmed publisher
Ao Y, Mich Basso J, Lin B, Yang L. High efficient differentiation of functional hepatocytes from porcine induced pluripotent stem cells. PLoS ONE. 2014;9:e100417 pubmed publisher
Ménard L, Parker P, Kermorgant S. Receptor tyrosine kinase c-Met controls the cytoskeleton from different endosomes via different pathways. Nat Commun. 2014;5:3907 pubmed publisher
Mitchell R, Szabo E, Shapovalova Z, Aslostovar L, Makondo K, Bhatia M. Molecular evidence for OCT4-induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors. Stem Cells. 2014;32:2178-87 pubmed publisher
Ekert J, Johnson K, Strake B, Pardinas J, Jarantow S, Perkinson R, et al. Three-dimensional lung tumor microenvironment modulates therapeutic compound responsiveness in vitro--implication for drug development. PLoS ONE. 2014;9:e92248 pubmed publisher
Kanteti R, El Hashani E, Dhanasingh I, Tretiakova M, Husain A, Sharma S, et al. Role of PAX8 in the regulation of MET and RON receptor tyrosine kinases in non-small cell lung cancer. BMC Cancer. 2014;14:185 pubmed publisher
Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, et al. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET. J Biol Chem. 2014;289:11068-82 pubmed publisher
Chattopadhyay C, Grimm E, Woodman S. Simultaneous inhibition of the HGF/MET and Erk1/2 pathways affect uveal melanoma cell growth and migration. PLoS ONE. 2014;9:e83957 pubmed publisher
Takayama K, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, Tachibana M, et al. Long-term self-renewal of human ES/iPS-derived hepatoblast-like cells on human laminin 111-coated dishes. Stem Cell Reports. 2013;1:322-35 pubmed publisher
Takayama K, Kawabata K, Nagamoto Y, Inamura M, Ohashi K, Okuno H, et al. CCAAT/enhancer binding protein-mediated regulation of TGF? receptor 2 expression determines the hepatoblast fate decision. Development. 2014;141:91-100 pubmed publisher
Yen B, Yen M, Hsu P, Liu K, Wang C, Bai C, et al. Multipotent human mesenchymal stromal cells mediate expansion of myeloid-derived suppressor cells via hepatocyte growth factor/c-met and STAT3. Stem Cell Reports. 2013;1:139-51 pubmed publisher
Chang W, Andrejecsk J, Kluger M, Saltzman W, Pober J. Pericytes modulate endothelial sprouting. Cardiovasc Res. 2013;100:492-500 pubmed publisher
Sugiyama N, Gucciardo E, Tatti O, Varjosalo M, Hyytiäinen M, Gstaiger M, et al. EphA2 cleavage by MT1-MMP triggers single cancer cell invasion via homotypic cell repulsion. J Cell Biol. 2013;201:467-84 pubmed publisher
Hasenauer S, Malinger D, Koschut D, Pace G, Matzke A, von Au A, et al. Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins. PLoS ONE. 2013;8:e62357 pubmed publisher
Gourlaouen M, Welti J, Vasudev N, Reynolds A. Essential role for endocytosis in the growth factor-stimulated activation of ERK1/2 in endothelial cells. J Biol Chem. 2013;288:7467-80 pubmed publisher
Bonanno G, Mariotti A, Procoli A, Folgiero V, Natale D, De Rosa L, et al. Indoleamine 2,3-dioxygenase 1 (IDO1) activity correlates with immune system abnormalities in multiple myeloma. J Transl Med. 2012;10:247 pubmed publisher
Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, et al. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells. Mol Ther. 2013;21:228-39 pubmed publisher
Furcht C, Muñoz Rojas A, Nihalani D, Lazzara M. Diminished functional role and altered localization of SHP2 in non-small cell lung cancer cells with EGFR-activating mutations. Oncogene. 2013;32:2346-55, 2355.e1-10 pubmed publisher
Tsai S, Bouwman B, Ang Y, Kim S, Lee D, Lemischka I, et al. Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells. Stem Cells. 2011;29:964-71 pubmed publisher
Evans I, Yamaji M, Britton G, Pellet Many C, Lockie C, Zachary I, et al. Neuropilin-1 signaling through p130Cas tyrosine phosphorylation is essential for growth factor-dependent migration of glioma and endothelial cells. Mol Cell Biol. 2011;31:1174-85 pubmed publisher
Ramani V, Yang Y, Ren Y, Nan L, Sanderson R. Heparanase plays a dual role in driving hepatocyte growth factor (HGF) signaling by enhancing HGF expression and activity. J Biol Chem. 2011;286:6490-9 pubmed publisher
Casazza A, Finisguerra V, Capparuccia L, Camperi A, Swiercz J, Rizzolio S, et al. Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice. J Clin Invest. 2010;120:2684-98 pubmed publisher
Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, et al. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Am J Physiol Cell Physiol. 2010;298:C465-76 pubmed publisher
Phin S, Marchand Adam S, Fabre A, Marchal Sommé J, Bantsimba Malanda C, Kataoka H, et al. Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice. Am J Respir Cell Mol Biol. 2010;42:286-93 pubmed publisher
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Schnickmann S, Camacho Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, et al. AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor. J Invest Dermatol. 2009;129:1140-8 pubmed publisher
Nicoleau C, Benzakour O, Agasse F, Thiriet N, Petit J, Prestoz L, et al. Endogenous hepatocyte growth factor is a niche signal for subventricular zone neural stem cell amplification and self-renewal. Stem Cells. 2009;27:408-19 pubmed publisher
Reed S, Johnson S. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types. J Cell Physiol. 2008;215:329-36 pubmed
Watanabe K, Bianco C, Strizzi L, Hamada S, Mancino M, Bailly V, et al. Growth factor induction of Cripto-1 shedding by glycosylphosphatidylinositol-phospholipase D and enhancement of endothelial cell migration. J Biol Chem. 2007;282:31643-55 pubmed
Skibinski G, Elborn J, Ennis M. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol. 2007;293:L69-76 pubmed
Kitajima T, Terai H, Ito Y. A fusion protein of hepatocyte growth factor for immobilization to collagen. Biomaterials. 2007;28:1989-97 pubmed
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Dong G, Lee T, Yeh N, Geoghegan J, Van Waes C, Chen Z. Metastatic squamous cell carcinoma cells that overexpress c-Met exhibit enhanced angiogenesis factor expression, scattering and metastasis in response to hepatocyte growth factor. Oncogene. 2004;23:6199-208 pubmed
image
image 1 :
R&D Systems 294-HG-005/CF image 1
Recombinant Human HGF Protein Bioactivity
product information
brand :
R&D Systems
master code :
294-HG/CF
SKU :
294-HG-005/CF
product name :
Recombinant Human HGF Protein, CF
description :
The Recombinant Human HGF Protein, CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human HGF Protein, CF has been validated for the following applications: Bioactivity.
target :
HGF
category :
Proteins and Enzymes
sizes available :
5 ug (also 25 ug, 100 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in NaH2PO4 and NaCl.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
60-70 kDa, nonreducing conditions 60 kDa, and 33-34 kDa, reducing conditions
theoretical molecular weight :
53.7 kDa ( alpha chain), 26 kDa ( beta chain)
gene symbol :
HGF
details of functionality :
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al . (1999) Blood 94 :3883. The ED50 for this effect is 0.1-0.5 ng/mL. The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/µg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P14210.2
applications :
Bioactivity
extended description :
An economically priced, mammalian-expressed Version Available!
source :
Sf 21 (baculovirus)-derived Recombinant Human HGF Protein, CF
USD :
362
USD 2023 :
362 USD
product details :
An economically priced, mammalian-expressed Version Available!
alt names :
deafness, autosomal recessive 39, DFNB39, EC 3.4.21, EC 3.4.21.7, fibroblast-derived tumor cytotoxic factor, F-TCF, hepatocyte growth factor (hepapoietin A; scatter factor), Hepatopoeitin-A, Hepatopoietin A, HGFB, HPTA, HPTAhepatocyte growth factor, lung fibroblast-derived mitogen, Scatter factor, SF, SFhepatopoeitin-A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.