Recombinant Human Granzyme A Protein, CF | R&D Systems 2905-SE-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Granzyme A Protein, CF

catalog :

2905-SE-010

quantity :

10 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Larimer B, Wehrenberg Klee E, Dubois F, Mehta A, Kalomeris T, Flaherty K, et al. Granzyme B PET Imaging as a Predictive Biomarker of Immunotherapy Response. Cancer Res. 2017;77:2318-2327 pubmed publisher

product information

master code :

2905-SE

SKU :

2905-SE-010

product name :

Recombinant Human Granzyme A Protein, CF

unit size :

10 ug

description :

The Recombinant Human Granzyme A Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Granzyme A Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Granzyme A

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in MES, NaCl and CaCl2 with Trehalose.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

Multiple bands between 29-33 kDa, reducing conditions

theoretical molecular weight :

28 kDa

gene symbol :

GZMA

details of functionality :

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5ÆDithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al . (1999) J. Biol. Chem. 274 :30468. The specific activity is 5,000 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P12544

applications :

Enzyme Activity

source long :

Mouse myeloma cell line, NS0-derived human Granzyme A protein Cys26-Val262, with a C-terminal 10-His tag

source short :

NS0

USD :

432 USD

alt names :

CTL tryptase, CTLA3, CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease), Cytotoxic T-lymphocyte proteinase 1, EC 3.4.21, fragmentin-1, granzyme A, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3), Granzyme-1, GZMA, H factor, Hanukkah factor, HF, HFSP, HFSPEC 3.4.21.78

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage