Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF | R&D Systems 288-TPN-025/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF

catalog :

288-TPN-025/CF

quantity :

25 ug

price :

377 USD

more info or order :

R&D Systems product webpage

citations: 6

Reference
Niwa A, Heike T, Umeda K, Oshima K, Kato I, Sakai H, et al. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE. 2011;6:e22261 pubmed publisher
Uchida N, Hsieh M, Hayakawa J, Madison C, Washington K, Tisdale J. Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther. 2011;18:1078-86 pubmed publisher
Schipper H, de Jager W, van Dijk M, Meerding J, Zelissen P, Adan R, et al. A multiplex immunoassay for human adipokine profiling. Clin Chem. 2010;56:1320-8 pubmed publisher
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, et al. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp Hematol. 2008;36:1121-31 pubmed publisher
Rajesh D, Chinnasamy N, Mitalipov S, Wolf D, Slukvin I, Thomson J, et al. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells. 2007;25:490-9 pubmed
Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed

image

image 1 :

Recombinant Human Thrombopoietin (NS0-expressed) Protein Bioactivity

product information

brand :

R&D Systems

master code :

288-TPN/CF

SKU :

288-TPN-025/CF

product name :

Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF

unit size :

25 ug

description :

GluN2B-selective NMDA antagonist; also agonist

target :

Thrombopoietin/Tpo

category :

Proteins and Enzymes

alt names :

Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

all available conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

observed molecular weight :

80-90 kDa, reducing conditions

theoretical molecular weight :

35 kDa

gene symbol :

THPO

details of functionality :

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al . (1988) Br. J. Haematol. 69 :359. The ED50 for this effect is 5-15 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P40225

applications :

Bioactivity

source :

NS0-derived Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF

extended description :

A new economical version of TPO made in E. coli is now available.

USD :

349

Jul-22 :

377 USD

product details :

A new economical version of TPO made in E. coli is now available.

sizes available :

25 ug (also 10 ug, 100 ug)

more info or order :

R&D Systems product webpage