product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF
catalog :
288-TPN-025/CF
quantity :
25 ug (also 10 ug, 100 ug)
price :
400 USD
more info or order :
R&D Systems product webpage
citations: 12
Reference
Kunitomi A, Hirohata R, Arreola V, Osawa M, Kato T, Nomura M, et al. Improved Sendai viral system for reprogramming to naive pluripotency. Cell Rep Methods. 2022;2:100317 pubmed publisher
Sudo K, Yamazaki S, Wilkinson A, Nakauchi H, Nakamura Y. Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo. Stem Cell Res. 2021;56:102531 pubmed publisher
Aerts Kaya F, Visser T, Pervin B, Mammadova A, Ozyuncu O, Wagemaker G, et al. SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential. Biol Blood Marrow Transplant. 2020;26:634-642 pubmed publisher
Ohta R, Sugimura R, Niwa A, Saito M. Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition. J Vis Exp. 2019;: pubmed publisher
Niwa A, Heike T, Umeda K, Oshima K, Kato I, Sakai H, et al. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE. 2011;6:e22261 pubmed publisher
Uchida N, Hsieh M, Hayakawa J, Madison C, Washington K, Tisdale J. Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther. 2011;18:1078-86 pubmed publisher
Schipper H, de Jager W, van Dijk M, Meerding J, Zelissen P, Adan R, et al. A multiplex immunoassay for human adipokine profiling. Clin Chem. 2010;56:1320-8 pubmed publisher
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, et al. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp Hematol. 2008;36:1121-31 pubmed publisher
Cheli Y, Kanaji S, Jacquelin B, Chang M, Nugent D, Kunicki T. Transcriptional and epigenetic regulation of the integrin collagen receptor locus ITGA1-PELO-ITGA2. Biochim Biophys Acta. 2007;1769:546-58 pubmed
Rajesh D, Chinnasamy N, Mitalipov S, Wolf D, Slukvin I, Thomson J, et al. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells. 2007;25:490-9 pubmed
Kanaji S, Kanaji T, Jacquelin B, Chang M, Nugent D, Komatsu N, et al. Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation. Blood. 2005;105:3888-92 pubmed
Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed
product information
brand :
R&D Systems
catalog number base :
288-TPN/CF
SKU :
288-TPN-025/CF
product name :
Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF
units size :
25 ug (also 10 ug, 100 ug)
description :
The Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF has been validated for the following applications: Bioactivity.
target :
Thrombopoietin/Tpo
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
all_available conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
80-90 kDa, reducing conditions
theoretical molecular weight :
35 kDa
gene symbol :
THPO
details of functionality :
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al . (1988) Br. J. Haematol. 69 :359. The ED50 for this effect is 5-15 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
top caption :
Recombinant Human Thrombopoietin (NS0-expressed) Protein Bioactivity
accessionNumbers :
P40225
applications :
Bioactivity
source :
NS0-derived Recombinant Human Thrombopoietin (NS0-expressed) Protein, CF
USD :
400 USD
alt names :
Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.