product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Thrombopoietin Protein
catalog :
288-TP-005
quantity :
5 ug (also 25 ug, 200 ug, 1 mg)
price :
363 USD
more info or order :
citations: 90
Reference
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Uchida N, Nassehi T, Drysdale C, Gamer J, Yapundich M, Demirci S, et al. High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2. Mol Ther Methods Clin Dev. 2019;13:187-196 pubmed publisher
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Huang X, Guo B, Liu S, Wan J, Broxmeyer H. Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment. Nat Commun. 2018;9:2741 pubmed publisher
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Tkaczynski E, Arulselvan A, Tkaczynski J, Avery S, Xiao L, Torok Storb B, et al. 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia. Blood Adv. 2018;2:754-761 pubmed publisher
Zheng S, Papalexi E, Butler A, STEPHENSON W, Satija R. Molecular transitions in early progenitors during human cord blood hematopoiesis. Mol Syst Biol. 2018;14:e8041 pubmed publisher
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Palpant N, Wang Y, Hadland B, Zaunbrecher R, Redd M, Jones D, et al. Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis. Cell Rep. 2017;20:1597-1608 pubmed publisher
Lee J, Salci K, Reid J, Orlando L, Tanasijevic B, Shapovalova Z, et al. Brief Report: Human Acute Myeloid Leukemia Reprogramming to Pluripotency Is a Rare Event and Selects for Patient Hematopoietic Cells Devoid of Leukemic Mutations. Stem Cells. 2017;35:2095-2102 pubmed publisher
Huang J, Khan A, Au B, Barber D, Lopez Vasquez L, Prokopishyn N, et al. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34+ Cells for Correction of Fabry Disease. Mol Ther Methods Clin Dev. 2017;5:241-258 pubmed publisher
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Guo B, Huang X, Cooper S, Broxmeyer H. Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment. Nat Med. 2017;23:424-428 pubmed publisher
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Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, et al. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML). PLoS ONE. 2015;10:e0122983 pubmed publisher
Baldwin K, Urbinati F, Romero Z, Campo Fernandez B, Kaufman M, Cooper A, et al. Enrichment of human hematopoietic stem/progenitor cells facilitates transduction for stem cell gene therapy. Stem Cells. 2015;33:1532-42 pubmed publisher
Shaham L, Vendramini E, Ge Y, Goren Y, Birger Y, Tijssen M, et al. MicroRNA-486-5p is an erythroid oncomiR of the myeloid leukemias of Down syndrome. Blood. 2015;125:1292-301 pubmed publisher
Barese C, Felizardo T, Sellers S, Keyvanfar K, Di Stasi A, Metzger M, et al. Regulated apoptosis of genetically modified hematopoietic stem and progenitor cells via an inducible caspase-9 suicide gene in rhesus macaques. Stem Cells. 2015;33:91-100 pubmed publisher
Shen S, Xu N, Klamer G, Ko K, Khoo M, Ma D, et al. Small-molecule inhibitor of glycogen synthase kinase 3β 6-Bromoindirubin-3-oxime inhibits hematopoietic regeneration in stem cell recipient mice. Stem Cells Dev. 2015;24:724-36 pubmed publisher
Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, et al. Targeted 25-hydroxyvitamin D3 1α-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice. Mol Ther. 2015;23:339-51 pubmed publisher
Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, et al. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies. Sci Rep. 2014;4:6136 pubmed publisher
Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, et al. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood. 2014;124:1393-403 pubmed publisher
Rosen D, Leung L, Louie B, Cordeiro J, Conroy A, Shapira I, et al. Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP). J Transl Med. 2014;12:184 pubmed publisher
Chaurasia P, Gajzer D, Schaniel C, D Souza S, Hoffman R. Epigenetic reprogramming induces the expansion of cord blood stem cells. J Clin Invest. 2014;124:2378-95 pubmed publisher
Wu C, Li B, Lu R, Koelle S, Yang Y, Jares A, et al. Clonal tracking of rhesus macaque hematopoiesis highlights a distinct lineage origin for natural killer cells. Cell Stem Cell. 2014;14:486-499 pubmed publisher
Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, et al. Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell. 2014;14:535-48 pubmed publisher
Kamat V, Paluru P, Myint M, French D, Gadue P, Diamond S. MicroRNA screen of human embryonic stem cell differentiation reveals miR-105 as an enhancer of megakaryopoiesis from adult CD34+ cells. Stem Cells. 2014;32:1337-46 pubmed publisher
Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, et al. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood. 2014;123:1239-49 pubmed publisher
Sjeklocha L, Wong P, Belcher J, Vercellotti G, Steer C. ?-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model. PLoS ONE. 2013;8:e80403 pubmed publisher
Uchida N, Weitzel R, Evans M, Green R, Bonifacino A, Krouse A, et al. Evaluation of engraftment and immunological tolerance after reduced intensity conditioning in a rhesus hematopoietic stem cell gene therapy model. Gene Ther. 2014;21:148-57 pubmed publisher
Csaszar E, Wang W, Usenko T, Qiao W, Delaney C, Bernstein I, et al. Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling. Blood. 2014;123:650-8 pubmed publisher
Shinohara A, Imai Y, Nakagawa M, Takahashi T, Ichikawa M, Kurokawa M. Intracellular reactive oxygen species mark and influence the megakaryocyte-erythrocyte progenitor fate of common myeloid progenitors. Stem Cells. 2014;32:548-57 pubmed publisher
Doulatov S, Vo L, Chou S, Kim P, Arora N, Li H, et al. Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via respecification of lineage-restricted precursors. Cell Stem Cell. 2013;13:459-70 pubmed publisher
Dasouki M, Rafi S, Olm Shipman A, Wilson N, Abhyankar S, Ganter B, et al. Exome sequencing reveals a thrombopoietin ligand mutation in a Micronesian family with autosomal recessive aplastic anemia. Blood. 2013;122:3440-9 pubmed publisher
Giannoni F, Hardee C, Wherley J, Gschweng E, Senadheera S, Kaufman M, et al. Allelic exclusion and peripheral reconstitution by TCR transgenic T cells arising from transduced human hematopoietic stem/progenitor cells. Mol Ther. 2013;21:1044-54 pubmed publisher
Lee M, Prasain N, Chae H, Kim Y, Mantel C, Yoder M, et al. Epigenetic regulation of NANOG by miR-302 cluster-MBD2 completes induced pluripotent stem cell reprogramming. Stem Cells. 2013;31:666-81 pubmed publisher
Jawad M, Yu N, Seedhouse C, Tandon K, Russell N, Pallis M. Targeting of CD34+CD38- cells using Gemtuzumab ozogamicin (Mylotarg) in combination with tipifarnib (Zarnestra) in Acute Myeloid Leukaemia. BMC Cancer. 2012;12:431 pubmed publisher
Kodama T, Hikita H, Kawaguchi T, Shigekawa M, Shimizu S, Hayashi Y, et al. Mcl-1 and Bcl-xL regulate Bak/Bax-dependent apoptosis of the megakaryocytic lineage at multistages. Cell Death Differ. 2012;19:1856-69 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Bauer T, Olson E, Huo Y, Tuschong L, Allen J, Li Y, et al. Treatment of canine leukocyte adhesion deficiency by foamy virus vectors expressing CD18 from a PGK promoter. Gene Ther. 2011;18:553-9 pubmed publisher
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Srivastava A, Nedelcu E, Esmaeli Azad B, Mishra R, Carrier E. Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells. Stem Cells. 2007;25:1456-61 pubmed
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Kurita R, Sasaki E, Yokoo T, Hiroyama T, Takasugi K, Imoto H, et al. Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells. Stem Cells. 2006;24:2014-22 pubmed
Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, et al. Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells. Exp Hematol. 2006;34:760-9 pubmed
Kahl C, Tarantal A, Lee C, Jimenez D, Choi C, Pepper K, et al. Effects of busulfan dose escalation on engraftment of infant rhesus monkey hematopoietic stem cells after gene marking by a lentiviral vector. Exp Hematol. 2006;34:369-81 pubmed
Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, et al. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells. Cell Death Differ. 2006;13:1686-96 pubmed
Corazza F, Hermans C, D Hondt S, Ferster A, Kentos A, Benoit Y, et al. Circulating thrombopoietin as an in vivo growth factor for blast cells in acute myeloid leukemia. Blood. 2006;107:2525-30 pubmed
Drouet M, Mathieu J, Grenier N, Multon E, Sotto J, Herodin F. The reduction of in vitro radiation-induced Fas-related apoptosis in CD34+ progenitor cells by SCF, FLT-3 ligand, TPO, and IL-3 in combination resulted in CD34+ cell proliferation and differentiation. Stem Cells. 1999;17:273-85 pubmed
product information
master code :
288-TP
SKU :
288-TP-005
product name :
Recombinant Human Thrombopoietin Protein
unit size :
5 ug (also 25 ug, 200 ug, 1 mg)
description :
The Recombinant Human Thrombopoietin Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human Thrombopoietin Protein has been validated for the following applications: Bioactivity.
target :
Thrombopoietin/Tpo
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE with silver staining
species :
Human
observed molecular weight :
43-60 kDa, reducing conditions
theoretical molecular weight :
35 kDa
gene symbol :
THPO
details of functionality :
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al . (1988) Br. J. Haematol. 69 :359. The ED50 for this effect is 0.3-3 ng/mL.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human Thrombopoietin Protein Bioactivity
accessionNumbers :
P40225
applications :
Bioactivity
source long :
Spodoptera frugiperda , Sf 21 (baculovirus)-derived human Thrombopoietin/Tpo protein Ser22-Gly353
source short :
Sf 21 (baculovirus)
USD :
363 USD
alt names :
Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.