product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
catalog :
287-TC-500
quantity :
500 UN
price :
464 USD
more info or order :
citations: 61
Reference
Michaels Y, Edgar J, Major M, Castle E, Zimmerman C, Yin T, et al. DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells. Sci Adv. 2022;8:eabn5522 pubmed publisher
Ramshekar A, Bretz C, Kunz E, Cung T, Richards B, Stoddard G, et al. Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization. Biomedicines. 2022;10: pubmed publisher
Luff S, Creamer J, Valsoni S, Dege C, Scarf xf2 R, Dacunto A, et al. Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells. Nat Cell Biol. 2022;24:616-624 pubmed publisher
Iannello G, Patel A, Sirabella D, Corneo B, Thaker V. Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL. Stem Cell Res. 2021;59:102635 pubmed publisher
de Souza A, Bressan F, Pieri N, Botigelli R, Revay T, Haddad S, et al. Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients. Cells. 2021;10: pubmed publisher
Wegscheid M, Anastasaki C, Hartigan K, Cobb O, Papke J, Traber J, et al. Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis. Cell Rep. 2021;36:109315 pubmed publisher
Iannello G, Patel A, Sirabella D, Corneo B, Thaker V. Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity. Stem Cell Res. 2021;54:102432 pubmed publisher
Li Z, Zhang X, Li X, Yang Y, Xin H, Yang X, et al. A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene. Stem Cell Res. 2021;53:102313 pubmed publisher
Borsoi J, Morato Marques M, de Araújo Tofoli F, Assis Pereira L, Farinha Arcieri L, Delgado Sarafian R, et al. Generation of two human induced pluripotent stem cell (hiPSC) lines derived from unrelated Marfan Syndrome patients. Stem Cell Res. 2021;54:102407 pubmed publisher
Zhang H, Liu C, Ma Y, Lin L, Lv Y, Gao M, et al. Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene. Stem Cell Res. 2021;53:102331 pubmed publisher
Yang X, Liu N, Mu H, Lv Y, Zhang H, Li Y, et al. Reprogramming of human peripheral blood mononuclear cell (PBMC) from a patient suffering from hearing loss into iPSC line (SDQLCHi035-A) maintaining compound heterozygous variations in GJB2 gene. Stem Cell Res. 2021;51:102188 pubmed publisher
Yang X, Duan C, Zhang H, Li Y, Guan J, Wang D, et al. Establishment of a non-integrate iPS cell line (SDQLCHi023-A) from a patient with Xq25 microduplication syndrome carrying a 1.3 Mb hemizygote duplication at chrXq25. Stem Cell Res. 2021;51:102147 pubmed publisher
Wang B, Yang L, Li Y, Gao M, Zhang H, Yang X, et al. Establishment of a human induced pluripotent stem cell line (SDQLCHi037-A) from a patient with Alagille syndrome carrying heterozygous mutation in JAG1 gene. Stem Cell Res. 2021;51:102162 pubmed publisher
Zhang H, Zhang H, Ma Y, Lv Y, Gai Z, Liu Y. Generation and characterization of an induced pluripotent stem cell line SDQLCHi018-A from a congenital myasthenic syndrome patient carrying compound heterozygote mutations in RAPSN gene. Stem Cell Res. 2021;51:102160 pubmed publisher
Liu N, Yang X, Wang S, Dong R, Li Y, Lv Y, et al. PBMC-derived integration-free iPSCs line SDQLCHi039-A from a patient with X-linked agammaglobulinemia carrying a novel 9-bp in-frame deletion in BTK gene. Stem Cell Res. 2021;51:102165 pubmed publisher
Wang B, Liu C, Zhang H, Gai Z, Liu Y. An induced pluripotent stem cell line (SDQLCHi033-A) derived from a patient with maple syrup urine disease type Ib carrying a homozygous mutation in BCKDHB gene. Stem Cell Res. 2020;50:102146 pubmed publisher
Riccetti S, Sinigaglia A, Desole G, Nowotny N, Trevisan M, Barzon L. Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells. Viruses. 2020;12: pubmed publisher
Yu H, Hu W, Song X, Descalzi Montoya D, Yang Z, Korngold R, et al. Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria. Int J Mol Sci. 2020;21: pubmed publisher
Brendel C, Negre O, Rothe M, Guda S, Parsons G, Harris C, et al. Preclinical Evaluation of a Novel Lentiviral Vector Driving Lineage-Specific BCL11A Knockdown for Sickle Cell Gene Therapy. Mol Ther Methods Clin Dev. 2020;17:589-600 pubmed publisher
Liu X, Yang X, Li Y, Wang X, Ma J, Jiang W, et al. Generation of patient-specific pluripotent induced stem cell line SDUBMSI002-A from a patient with X-linked mental retardation syndrome. Stem Cell Res. 2020;43:101724 pubmed publisher
Ma Y, Zhang H, Li X, Yang X, Li Y, Guan J, et al. An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene. Stem Cell Res. 2020;43:101729 pubmed publisher
Guan J, Li Z, Zhang H, Yang X, Ma Y, Li Y, et al. Generation of a Human iPSC line (SDQLCHi021-A) from a patient with methylmalonic acidemia cblC type carrying compound heterozygous mutations in MMAHC gene. Stem Cell Res. 2020;43:101709 pubmed publisher
Xiaojing W, Yanyan M, Ruonan D, Xiaolin L, Haiyan Z, Jian M, et al. Generation of a human induced pluripotent stem cell line (SDUBMSi001-A) from a hereditary spastic paraplegia patient carrying kif1a c.773C>T missense mutation. Stem Cell Res. 2020;43:101727 pubmed publisher
Guan J, Liu X, Zhang H, Yang X, Ma Y, Li Y, et al. Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49-50 exons in the DMD gene. Stem Cell Res. 2020;42:101666 pubmed publisher
Montoya J, Turnidge M, Wai D, Patel A, Lee D, Gokhale V, et al. In vitro activity of a G-quadruplex-stabilizing small molecule that synergizes with Navitoclax to induce cytotoxicity in acute myeloid leukemia cells. BMC Cancer. 2019;19:1251 pubmed publisher
Zhang H, Ma Y, Li X, Yang X, Li Y, Guan J, et al. An integration-free iPSC line (SDQLCHi013-A) derived from a patient with maple syrup urine disease carrying compound heterozygote mutations in BCKDHA gene. Stem Cell Res. 2019;41:101585 pubmed publisher
Zhang H, Ma Y, Yu S, Yang X, Li Y, Guan J, et al. Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene. Stem Cell Res. 2019;41:101611 pubmed publisher
Guan J, Liu X, Zhang H, Lv Y, Wang X, Yang X, et al. Generation of an iPSC line (SDQLCHi015-A) from peripheral blood mononuclear cells of a patient with mental retardation type 15 carrying c.1007_1011del, p.(Ile336fs) in CUL4B gene. Stem Cell Res. 2019;41:101628 pubmed publisher
Ma Y, Zhang H, Zhang S, Dong R, Yang X, Li Y, et al. An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene. Stem Cell Res. 2019;41:101577 pubmed publisher
Li Y, Zhang H, Yan B, Ma Y, Yang X, Guan J, et al. An induced pluripotent stem cell line (SDQLCHi006-A) derived from a patient with maple syrup urine disease type Ib carrying compound heterozygous mutations of p.R168C and p.T322I in BCKDHB gene. Stem Cell Res. 2019;40:101579 pubmed publisher
Ma Y, Zhang H, Yang X, Li Y, Guan J, Lv Y, et al. Establishment of a human induced pluripotent stem cell line (SDQLCHi004-A) from a patient with nemaline myopathy-4 disease carrying heterozygous mutation in TPM2 gene. Stem Cell Res. 2019;40:101559 pubmed publisher
Kittur F, Lin Y, Arthur E, Hung C, Li P, Sane D, et al. Recombinant asialoerythropoetin protects HL-1 cardiomyocytes from injury via suppression of Mst1 activation. Biochem Biophys Rep. 2019;17:157-168 pubmed publisher
Cyganek L, Tiburcy M, Sekeres K, Gerstenberg K, Bohnenberger H, Lenz C, et al. Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes. JCI Insight. 2018;3: pubmed publisher
Allenby M, Tahlawi A, Morais J, Li K, Panoskaltsis N, Mantalaris A. Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids. Stem Cells Int. 2018;2018:6230214 pubmed publisher
Nagase R, Inoue D, Pastore A, Fujino T, Hou H, Yamasaki N, et al. Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation. J Exp Med. 2018;215:1729-1747 pubmed publisher
Han S, Lee E, Choi H, Chun B, Baek E. The effects of plasma gelsolin on human erythroblast maturation for erythrocyte production. Stem Cell Res. 2018;29:64-75 pubmed publisher
Ferrer C, Alders M, Postma A, Park S, Klein M, Cetinbaş M, et al. An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality. Genes Dev. 2018;32:373-388 pubmed publisher
Yoon H, Zhang X, Kang M, Kim G, Shin S, Baek S, et al. Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion. Int J Mol Sci. 2018;19: pubmed publisher
Miao S, Wang S, Cheng X, Li Y, Zhang Q, Li G, et al. Erythropoietin promoted the proliferation of hepatocellular carcinoma through hypoxia induced translocation of its specific receptor. Cancer Cell Int. 2017;17:119 pubmed publisher
Rahman N, Brauer P, Ho L, Usenko T, Tewary M, Zuniga Pflucker J, et al. Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence. Nat Commun. 2017;8:15380 pubmed publisher
Arthur E, Kittur F, Lin Y, Hung C, Sane D, Xie J. Plant-Produced Asialo-Erythropoietin Restores Pancreatic Beta-Cell Function by Suppressing Mammalian Sterile-20-like Kinase (MST1) and Caspase-3 Activation. Front Pharmacol. 2017;8:208 pubmed publisher
Derosa F, Guild B, Karve S, Smith L, Love K, Dorkin J, et al. Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system. Gene Ther. 2016;23:699-707 pubmed publisher
Canli Ã, AlankuÅŸ Y, Grootjans S, Vegi N, Hültner L, Hoppe P, et al. Glutathione peroxidase 4 prevents necroptosis in mouse erythroid precursors. Blood. 2016;127:139-48 pubmed publisher
Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, et al. Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation. Stem Cells. 2015;33:1470-9 pubmed publisher
Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, et al. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor. Stem Cells. 2015;33:976-87 pubmed publisher
Kernytsky A, Wang F, Hansen E, Schalm S, Straley K, Gliser C, et al. IDH2 mutation-induced histone and DNA hypermethylation is progressively reversed by small-molecule inhibition. Blood. 2015;125:296-303 pubmed publisher
Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, et al. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood. 2014;124:1393-403 pubmed publisher
Bu Y, Su F, Wang X, Gao H, Lei L, Chang N, et al. Protein tyrosine phosphatase PTPN9 regulates erythroid cell development through STAT3 dephosphorylation in zebrafish. J Cell Sci. 2014;127:2761-70 pubmed publisher
Vannucchi A, Rotunno G, Bartalucci N, Raugei G, Carrai V, Balliu M, et al. Calreticulin mutation-specific immunostaining in myeloproliferative neoplasms: pathogenetic insight and diagnostic value. Leukemia. 2014;28:1811-8 pubmed publisher
Ashino S, Takeda K, Li H, Taylor V, Joetham A, Pine P, et al. Janus kinase 1/3 signaling pathways are key initiators of TH2 differentiation and lung allergic responses. J Allergy Clin Immunol. 2014;133:1162-74 pubmed publisher
Sjeklocha L, Wong P, Belcher J, Vercellotti G, Steer C. ?-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model. PLoS ONE. 2013;8:e80403 pubmed publisher
Huang X, Shah S, Wang J, Ye Z, Dowey S, Tsang K, et al. Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors. Mol Ther. 2014;22:451-463 pubmed publisher
Ma L, Clayton J, Walgren R, Zhao B, Evans R, Smith M, et al. Discovery and characterization of LY2784544, a small-molecule tyrosine kinase inhibitor of JAK2V617F. Blood Cancer J. 2013;3:e109 pubmed publisher
McCloskey M, Wang H, Jiang Y, Smith G, Strange J, Hartnett M. Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity. Invest Ophthalmol Vis Sci. 2013;54:2020-6 pubmed publisher
Ichii M, Frank M, Iozzo R, Kincade P. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood. 2012;119:1683-92 pubmed publisher
Purandare A, McDevitt T, Wan H, You D, Penhallow B, Han X, et al. Characterization of BMS-911543, a functionally selective small-molecule inhibitor of JAK2. Leukemia. 2012;26:280-8 pubmed publisher
Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, et al. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp Hematol. 2008;36:1035-46 pubmed publisher
Braun B, Archard J, Van Ziffle J, Tuveson D, Jacks T, Shannon K. Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood. 2006;108:2041-4 pubmed
Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, et al. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells. Cell Death Differ. 2006;13:1686-96 pubmed
Hanley M, Napolitano L, McCune J. Growth hormone-induced stimulation of multilineage human hematopoiesis. Stem Cells. 2005;23:1170-9 pubmed
Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed
image
image 1 :
R&D Systems 287-TC-500 image 1
Graph showing dose-dependent bioactivity of Human Erythropoietin/ EPO Protein
product information
brand :
R&D Systems
master code :
287-TC
SKU :
287-TC-500
product name :
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
description :
The Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) from R&D Systems is derived from CHO. The Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) has been validated for the following applications: Bioactivity.
target :
Erythropoietin/EPO
category :
Proteins and Enzymes
sizes available :
500 UN
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
species :
Human
observed molecular weight :
37 kDa, reducing conditions
theoretical molecular weight :
21 kDa
gene symbol :
EPO
details of functionality :
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al . (1972) Bull. Hlth. Org. 47 :99. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 0.015-0.075 units/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
CAA26094
applications :
Bioactivity
source :
CHO-derived Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
USD :
464
USD 2023 :
464 USD
alt names :
ECYT5, EP, EPO, epoetin, erythropoietin, MGC138142, MVCD2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt at -20 to -70 °C as supplied. 1 month at 2-8 °C under sterile conditions after reconstitution. 3 months at -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.