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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse IL-27 (NS0-expressed) Protein, CF
catalog :
2799-ML-010/CF
quantity :
10 ug
price :
432 USD
more info or order :
R&D Systems product webpage
citations: 38
Reference
Kim D, Kim S, Kang M, Yin Z, Min B. Cell type specific IL-27p28 (IL-30) deletion in mice uncovers an unexpected regulatory function of IL-30 in autoimmune inflammation. Sci Rep. 2023;13:1812 pubmed publisher
Choi G, Park Y, Cho M, Moon H, Kim D, Kang C, et al. A critical role for Th17 cell-derived TGF-β1 in regulating the stability and pathogenicity of autoimmune Th17 cells. Exp Mol Med. 2021;53:993-1004 pubmed publisher
Luo X, Zeng Q, Li Y, Tang Y, Liu W, Luo R. The Inhibition of Group II Innate Lymphoid Cell Response by IL-27 in Allergic Rhinitis. J Immunol Res. 2020;2020:6661524 pubmed publisher
Zhang H, Madi A, Yosef N, Chihara N, Awasthi A, Pot C, et al. An IL-27-Driven Transcriptional Network Identifies Regulators of IL-10 Expression across T Helper Cell Subsets. Cell Rep. 2020;33:108433 pubmed publisher
Choi G, Na H, Kuen D, Kim B, Chung Y. Autocrine TGF-β1 Maintains the Stability of Foxp3+ Regulatory T Cells via IL-12Rβ2 Downregulation. Biomolecules. 2020;10: pubmed publisher
Matsuda M, Doi K, Tsutsumi T, Inaba M, Hamaguchi J, Terada T, et al. Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice. J Pharmacol Sci. 2019;141:139-145 pubmed publisher
Abdullah A, Maged M, Hairul Islam M I, Osama I A, Maha H, Manal A, et al. Activation of aryl hydrocarbon receptor signaling by a novel agonist ameliorates autoimmune encephalomyelitis. PLoS ONE. 2019;14:e0215981 pubmed publisher
Nguyen Q, Jang E, Le H, Kim S, Kim D, Dvorina N, et al. IL-27 targets Foxp3+ Tregs to mediate antiinflammatory functions during experimental allergic airway inflammation. JCI Insight. 2019;4: pubmed publisher
Aoki R, Aoki Yoshida A, Suzuki C, Takayama Y. Indole-3-Pyruvic Acid, an Aryl Hydrocarbon Receptor Activator, Suppresses Experimental Colitis in Mice. J Immunol. 2018;201:3683-3693 pubmed publisher
Ting H, de Almeida Nagata D, Rasky A, Malinczak C, Maillard I, Schaller M, et al. Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection. Mucosal Immunol. 2018;11:1524-1536 pubmed publisher
Bouchareychas L, Grössinger E, Kang M, Adamopoulos I. ??TCR regulates production of interleukin-27 by neutrophils and attenuates inflammatory arthritis. Sci Rep. 2018;8:7590 pubmed publisher
Do J, Kim D, Kim S, Valentin Torres A, Dvorina N, Jang E, et al. Treg-specific IL-27R? deletion uncovers a key role for IL-27 in Treg function to control autoimmunity. Proc Natl Acad Sci U S A. 2017;114:10190-10195 pubmed publisher
Carson W, Cavassani K, Soares E, Hirai S, Kittan N, Schaller M, et al. The STAT4/MLL1 Epigenetic Axis Regulates the Antimicrobial Functions of Murine Macrophages. J Immunol. 2017;199:1865-1874 pubmed publisher
Mufazalov I, Kuschmann J, Andruszewski D, Masri J, Gabriel L, Adams P, et al. Balanced Bcl-3 expression in murine CD4+ T cells is required for generation of encephalitogenic Th17 cells. Eur J Immunol. 2017;47:1335-1341 pubmed publisher
Stier M, Goleniewska K, Cephus J, Newcomb D, Sherrill T, Boyd K, et al. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. J Immunol. 2017;199:510-519 pubmed publisher
Pérez Cabezas B, Cecílio P, Robalo A, Silvestre R, Carrillo E, Moreno J, et al. Interleukin-27 Early Impacts Leishmania infantum Infection in Mice and Correlates with Active Visceral Disease in Humans. Front Immunol. 2016;7:478 pubmed
Vijayan D, Mohd Redzwan N, Avery D, Wirasinha R, Brink R, Walters G, et al. IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice. J Immunol. 2016;197:3008-3017 pubmed
Howes A, Taubert C, Blankley S, Spink N, Wu X, Graham C, et al. Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages. J Immunol. 2016;197:2838-53 pubmed publisher
Gao F, Yang Y, Feng X, Fan T, Jiang L, Guo R, et al. Interleukin-27 is elevated in sepsis-induced myocardial dysfunction and mediates inflammation. Cytokine. 2016;88:1-11 pubmed publisher
Tang H, Zhang J, Sun X, Qian X, Zhang Y, Jin R. Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes. Sci Rep. 2016;6:30448 pubmed publisher
Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, et al. TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun. 2015;6:6329 pubmed publisher
Zhu C, Sakuishi K, Xiao S, Sun Z, Zaghouani S, Gu G, et al. An IL-27/NFIL3 signalling axis drives Tim-3 and IL-10 expression and T-cell dysfunction. Nat Commun. 2015;6:6072 pubmed publisher
Neumann C, Heinrich F, Neumann K, Junghans V, Mashreghi M, Ahlers J, et al. Role of Blimp-1 in programing Th effector cells into IL-10 producers. J Exp Med. 2014;211:1807-19 pubmed publisher
de Aquino M, Kapil P, Hinton D, Phares T, Puntambekar S, Savarin C, et al. IL-27 limits central nervous system viral clearance by promoting IL-10 and enhances demyelination. J Immunol. 2014;193:285-94 pubmed publisher
Zhu E, Wang X, Zheng B, Wang Q, Hao J, Chen S, et al. miR-20b suppresses Th17 differentiation and the pathogenesis of experimental autoimmune encephalomyelitis by targeting ROR?t and STAT3. J Immunol. 2014;192:5599-609 pubmed publisher
Liu F, Kenngott E, Schröter M, Kühl A, Jennrich S, Watzlawick R, et al. Timed action of IL-27 protects from immunopathology while preserving defense in influenza. PLoS Pathog. 2014;10:e1004110 pubmed publisher
Sutherland A, Joller N, Michaud M, Liu S, Kuchroo V, Grusby M. IL-21 promotes CD8+ CTL activity via the transcription factor T-bet. J Immunol. 2013;190:3977-84 pubmed publisher
Tormo A, Letellier M, Sharma M, Elson G, Crabé S, Gauchat J. IL-6 activates STAT5 in T cells. Cytokine. 2012;60:575-82 pubmed publisher
Freeman B, Hammarlund E, Raué H, Slifka M. Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A. 2012;109:9971-6 pubmed publisher
Rajaiah R, Puttabyatappa M, Polumuri S, Moudgil K. Interleukin-27 and interferon-gamma are involved in regulation of autoimmune arthritis. J Biol Chem. 2011;286:2817-25 pubmed publisher
Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, et al. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J Virol. 2010;84:12703-12 pubmed publisher
Findlay E, Greig R, Stumhofer J, Hafalla J, de Souza J, Saris C, et al. Essential role for IL-27 receptor signaling in prevention of Th1-mediated immunopathology during malaria infection. J Immunol. 2010;185:2482-92 pubmed publisher
Pot C, Jin H, Awasthi A, Liu S, Lai C, Madan R, et al. Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells. J Immunol. 2009;183:797-801 pubmed publisher
Dibra D, Cutrera J, Xia X, Birkenbach M, Li S. Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance. Cancer Res. 2009;69:5505-13 pubmed publisher
Miyazaki Y, Shimanoe Y, Wang S, Yoshida H. Amelioration of delayed-type hypersensitivity responses by IL-27 administration. Biochem Biophys Res Commun. 2008;373:397-402 pubmed publisher
Guo B, Chang E, Cheng G. The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. J Clin Invest. 2008;118:1680-90 pubmed publisher
Fitzgerald D, Zhang G, El Behi M, Fonseca Kelly Z, Li H, Yu S, et al. Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells. Nat Immunol. 2007;8:1372-9 pubmed
Fitzgerald D, Ciric B, Touil T, Harle H, Grammatikopolou J, Das Sarma J, et al. Suppressive effect of IL-27 on encephalitogenic Th17 cells and the effector phase of experimental autoimmune encephalomyelitis. J Immunol. 2007;179:3268-75 pubmed
product information
master code :
2799-ML/CF
SKU :
2799-ML-010/CF
product name :
Recombinant Mouse IL-27 (NS0-expressed) Protein, CF
unit size :
10 ug
description :
The Recombinant Mouse IL-27 (NS0-expressed) Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse IL-27 (NS0-expressed) Protein, CF has been validated for the following applications: Bioactivity.
target :
IL-27
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS and CHAPS.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Mouse
observed molecular weight :
60-65 kDa, reducing conditions
theoretical molecular weight :
48.7 kDa
gene symbol :
Il27
details of functionality :
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al . (2009) Hepatology 50 :585. The ED50 for this effect is 0.6-6 ng/mL.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Mouse IL-27 (NS0-expressed) Protein Bioactivity
accessionNumbers :
O35228 (EBI3) Q8K3I6 (p28)
applications :
Bioactivity
source long :
Mouse myeloma cell line, NS0-derived mouse IL-27 protein Mouse EBI3 (Tyr19-Pro228) Accession # O35228 GGGSGGGSGGGSGGGS Mouse p28 (Phe29-Ser234) Accession # Q8K3I6 6-His tag N-terminus C-terminus
source short :
NS0
USD :
432 USD
alt names :
IL27, IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL27A, IL-27-A, IL27-A, IL27p28, IL30, interleukin 27, interleukin 30, interleukin-27 subunit alpha, MGC71873, p28IL-27A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.