Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) | R&D Systems 2706-CL-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged)

catalog :

2706-CL-025

quantity :

25 ug

price :

294 USD

more info or order :

R&D Systems product webpage

citations: 6

Reference
Rénier W, Bourdin A, Rubbo P, Peries M, Dedieu L, Bendriss S, et al. B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections. PLoS ONE. 2018;13:e0196470 pubmed publisher
Schoenhals M, Jourdan M, Bruyer A, Kassambara A, Klein B, Moreaux J. Hypoxia favors the generation of human plasma cells. Cell Cycle. 2017;16:1104-1117 pubmed publisher
Samuel J, Jayne S, Chen Y, Majid A, Wignall A, Wormull T, et al. Posttranscriptional Upregulation of p53 by Reactive Oxygen Species in Chronic Lymphocytic Leukemia. Cancer Res. 2016;76:6311-6319 pubmed
Cavallini C, Lovato O, Bertolaso A, Pacelli L, Zoratti E, Zanolin E, et al. The TNF-family cytokine TL1A inhibits proliferation of human activated B cells. PLoS ONE. 2013;8:e60136 pubmed publisher
Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje A, et al. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow. PLoS ONE. 2012;7:e35639 pubmed publisher
Celis E. Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction. Cancer. 2007;110:203-14 pubmed

image

image 1 :

Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) Bioactivity

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

2706-CL

SKU :

2706-CL-025

product name :

Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged)

Description :

The Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) from R&D Systems is derived from E. coli. The Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) has been validated for the following applications: Bioactivity.

target :

CD40 Ligand/TNFSF5

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and EDTA with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

18 kDa, reducing conditions

theoretical molecular weight :

17.7 kDa (monomer)

gene symbol :

CD40LG

details of functionality :

Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al . (1992) J. Exp. Med. 176 :1543. The ED50 for this effect is 2-12 ng/mL in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ) and 20 ng/mL of recombinant human IL-4.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P29965

applications :

Bioactivity

source :

E. coli-derived Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged)

USD :

294 USD

alt names :

CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage