product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human CCL3/MIP-1 alpha Protein, CF
catalog :
270-LD-010/CF
quantity :
10 ug (also 50 ug)
price :
291 USD
more info or order :
citations: 38
Reference
Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, et al. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience. 2021;24:103433 pubmed publisher
Karakaya B, van Moorsel C, Veltkamp M, Roodenburg Benschop C, Kazemier K, van der Helm van Mil A, et al. A Polymorphism in C-C Chemokine Receptor 5 (CCR5) Associates with Löfgren's Syndrome and Alters Receptor Expression as well as Functional Response. Cells. 2021;10: pubmed publisher
Ishida Y, Kuninaka Y, Nosaka M, Kimura A, Taruya A, Furuta M, et al. Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis. Nat Commun. 2020;11:5994 pubmed publisher
Kodama T, Koma Y, Arai N, Kido A, Urakawa N, Nishio M, et al. CCL3-CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways. Lab Invest. 2020;100:1140-1157 pubmed publisher
Nakano H, Kirino Y, Takeno M, Higashitani K, Nagai H, Yoshimi R, et al. GWAS-identified CCR1 and IL10 loci contribute to M1 macrophage-predominant inflammation in Behçet's disease. Arthritis Res Ther. 2018;20:124 pubmed publisher
Chen J, Liu X, Munshi M, Xu L, Tsakmaklis N, Demos M, et al. BTKCys481Ser drives ibrutinib resistance via ERK1/2, and protects BTKWild-Type MYD88 mutated cells by a paracrine mechanism. Blood. 2018;: pubmed publisher
van Attekum M, Terpstra S, Slinger E, von Lindern M, Moerland P, Jongejan A, et al. Macrophages confer survival signals via CCR1-dependent translational MCL-1 induction in chronic lymphocytic leukemia. Oncogene. 2017;36:3651-3660 pubmed publisher
Malard B, Florens N, Lambert C, Juillard L. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study. Artif Organs. 2017;41:545-555 pubmed publisher
Karlshøj S, Amarandi R, Larsen O, Daugvilaite V, Steen A, Brvar M, et al. Molecular Mechanism of Action for Allosteric Modulators and Agonists in CC-chemokine Receptor 5 (CCR5). J Biol Chem. 2016;291:26860-26874 pubmed publisher
Sips M, Krykbaeva M, Diefenbach T, Ghebremichael M, Bowman B, Dugast A, et al. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies. Mucosal Immunol. 2016;9:1584-1595 pubmed publisher
Gouwy M, De Buck M, Pörtner N, Opdenakker G, Proost P, Struyf S, et al. Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines. Eur J Immunol. 2015;45:101-12 pubmed publisher
Ravi A, Khurana S, Lemon J, Plumb J, Booth G, Healy L, et al. Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum. Respir Res. 2014;15:103 pubmed publisher
Buttari B, Profumo E, Domenici G, Tagliani A, Ippoliti F, Bonini S, et al. Neuropeptide Y induces potent migration of human immature dendritic cells and promotes a Th2 polarization. FASEB J. 2014;28:3038-49 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Badr G, Lefevre E, Mohany M. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE. 2011;6:e23741 pubmed publisher
Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J. CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection. Eur J Immunol. 2011;41:963-73 pubmed publisher
Schipper H, de Jager W, van Dijk M, Meerding J, Zelissen P, Adan R, et al. A multiplex immunoassay for human adipokine profiling. Clin Chem. 2010;56:1320-8 pubmed publisher
Leick M, Catusse J, Follo M, Nibbs R, Hartmann T, Veelken H, et al. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology. 2010;129:536-46 pubmed publisher
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J Leukoc Biol. 2008;84:1109-19 pubmed publisher
Bonecchi R, Borroni E, Anselmo A, Doni A, Savino B, Mirolo M, et al. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation. Blood. 2008;112:493-503 pubmed publisher
Biancotto A, Iglehart S, Lisco A, Vanpouille C, Grivel J, Lurain N, et al. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res Hum Retroviruses. 2008;24:453-62 pubmed publisher
Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, et al. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes. Blood. 2008;111:3579-90 pubmed publisher
Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
Pfleger C, Schloot N, ter Veld F. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J Immunol Methods. 2008;329:214-8 pubmed
Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, et al. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int J Cancer. 2008;122:769-76 pubmed
Grivel J, Elliott J, Lisco A, Biancotto A, Condack C, Shattock R, et al. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS. 2007;21:1263-72 pubmed
Jang S, Kim Y, Kim Y, Sung H, Ko J. Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1. J Biol Chem. 2007;282:11092-100 pubmed
Biancotto A, Grivel J, Iglehart S, Vanpouille C, Lisco A, Sieg S, et al. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood. 2007;109:4272-9 pubmed
Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, et al. Interaction between the CCR5 chemokine receptors and microbial HSP70. Eur J Immunol. 2006;36:2304-14 pubmed
Masih Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, et al. MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma. Blood. 2006;108:3465-71 pubmed
Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, et al. Novel antiviral activity of chemokines. Virology. 2006;350:484-92 pubmed
Dioszeghy V, Benlhassan Chahour K, Delache B, Dereuddre Bosquet N, Aubenque C, Gras G, et al. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression. J Virol. 2006;80:236-45 pubmed
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, et al. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J Immunol. 2004;173:6592-602 pubmed
Tay S, McCormack A, Rose M. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Transplantation. 2004;78:987-94 pubmed
Chang W, Baumgarth N, Yu D, Barry P. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J Virol. 2004;78:8720-31 pubmed
Hurst S, McLoughlin R, Monslow J, Owens S, Morgan L, Fuller G, et al. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J Immunol. 2002;169:5244-51 pubmed
Banisadr G, Queraud Lesaux F, Boutterin M, Pelaprat D, Zalc B, Rostene W, et al. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain. J Neurochem. 2002;81:257-69 pubmed
Lehner T, Wang Y, Doyle C, Tao L, Bergmeier L, Mitchell E, et al. Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques. Eur J Immunol. 1999;29:2427-35 pubmed
image
image 1 :
R&D Systems 270-LD-010/CF image 1
Recombinant Human CCL3/MIP-1 alpha Protein Bioactivity
product information
brand :
R&D Systems
master code :
270-LD/CF
SKU :
270-LD-010/CF
product name :
Recombinant Human CCL3/MIP-1 alpha Protein, CF
description :
The Recombinant Human CCL3/MIP-1 alpha Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human CCL3/MIP-1 alpha Protein, CF has been validated for the following applications: Bioactivity.
target :
CCL3/MIP-1 alpha
category :
Proteins and Enzymes
sizes available :
10 ug (also 50 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Human
theoretical molecular weight :
7.5 kDa
gene symbol :
CCL3
details of functionality :
Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 2-10 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 3-10 ng/mL.
endotoxin note :
<0.01 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P10147
applications :
Bioactivity
source :
E. coli-derived Recombinant Human CCL3/MIP-1 alpha Protein, CF
USD :
291 USD
alt names :
C-C motif chemokine 3, MIP1-(a), AI323804, chemokine (C-C motif) ligand 3, G0S19-1, LD78a, LD78alpha, MIP1 alpha, MIP-1 alpha, Mip1a, MIP-1alpha, MIP1-alpha, PAT 464.1, SCYA3, SIS-beta
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.