Recombinant Human Nidogen-1/Entactin Protein, CF | R&D Systems 2570-ND-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Nidogen-1/Entactin Protein, CF

catalog :

2570-ND-050

quantity :

50 ug

price :

525 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Viheriälä T, Sorvari J, Ihalainen T, Mörö A, Grönroos P, Schlie Wolter S, et al. Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. Sci Rep. 2021;11:933 pubmed publisher
Ferraro D, Patella F, Zanivan S, Donato C, Aceto N, Giannotta M, et al. Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells. BMC Cancer. 2019;19:312 pubmed publisher
Willumsen N, Bager C, Leeming D, Bay Jensen A, Karsdal M. Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer. Neoplasia. 2017;19:271-278 pubmed publisher
Toda M, Ueno M, Yamada J, Hiraga A, Tanaka H, Schlötzer Schrehardt U, et al. The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components. Invest Ophthalmol Vis Sci. 2016;57:4599-605 pubmed publisher
Mattsson J, Ravela S, Hekim C, Jonsson M, Malm J, NÃ¤rvÃ¤nen A, et al. Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity. PLoS ONE. 2014;9:e107819 pubmed publisher
Hendrickx A, van Luit Asbroek M, Schapendonk C, Van Wamel W, Braat J, Wijnands L, et al. SgrA, a nidogen-binding LPXTG surface adhesin implicated in biofilm formation, and EcbA, a collagen binding MSCRAMM, are two novel adhesins of hospital-acquired Enterococcus faecium. Infect Immun. 2009;77:5097-106 pubmed publisher
Zhang H, Tasnim F, Ying J, Zink D. The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys. Biomaterials. 2009;30:2899-911 pubmed publisher

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

2570-ND

SKU :

2570-ND-050

product name :

Recombinant Human Nidogen-1/Entactin Protein, CF

Description :

The Recombinant Human Nidogen-1/Entactin Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Nidogen-1/Entactin Protein, CF has been validated for the following applications: Bioactivity.

target :

Nidogen-1/Entactin

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

130 kDa, reducing conditions

theoretical molecular weight :

120 kDa

gene symbol :

NID1

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 4 x 104 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

AAH45606

applications :

Bioactivity

source :

NS0-derived Recombinant Human Nidogen-1/Entactin Protein

USD :

525 USD

alt names :

enactin, Entactin, Entactin-1, NID1, NID-1, NIDentactin, nidogen (enactin), nidogen 1, nidogen-1, Nidogen1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage