This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human CNTF Protein
catalog :
257-NT
quantity :
10 ug (also 50 ug)
price :
209 USD
citations: 27
Reference |
---|
Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, et al. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun. 2019;7:208 pubmed publisher
|
Sieving P, Caruso R, Tao W, Coleman H, Thompson D, Fullmer K, et al. Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants. Proc Natl Acad Sci U S A. 2006;103:3896-901 pubmed
|
He W, Gong K, Smith D, Ip N. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett. 2005;579:4317-23 pubmed
|
Fischer A, Omar G. Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in Müller glia in the chicken retina. J Comp Neurol. 2005;484:1-14 pubmed
|
Nielsen K, Chaverra M, Hapner S, Nelson B, Todd V, Zigmond R, et al. PACAP promotes sensory neuron differentiation: blockade by neurotrophic factors. Mol Cell Neurosci. 2004;25:629-41 pubmed
|
He W, Gong K, Zhu G, Smith D, Ip N. Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro. FEBS Lett. 2002;514:214-8 pubmed
|
product information
brand :
R&D Systems
master code :
257-NT
SKU :
257-NT-010
product name :
Recombinant Human CNTF Protein
description :
The Recombinant Human CNTF Protein from R&D Systems is derived from E. coli. The Recombinant Human CNTF Protein has been validated for the following applications: Bioactivity.
target :
CNTF
category :
Proteins and Enzymes
unit size :
10 ug (also 50 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
23-26 kDa, reducing conditions
theoretical molecular weight :
22.8 kDa
gene symbol :
CNTF
details of functionality :
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 0.03-0.18 µg/mL. The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR ) .
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
catalog number base :
257-NT
accessionNumbers :
P26441.1
applications :
Bioactivity
source :
E. coli -derived human CNTF protein Ala2-Met200
2020 USD :
209
2021 USD :
209 USD
alt names :
ciliary neurotrophic factor, HCNTF
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.
company information

R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.
questions and comments