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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human CNTF Protein
catalog :
257-NT-010
quantity :
10 ug (also 50 ug)
price :
252 USD
more info or order :
citations: 61
Reference
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Mashanov V, Alwan A, Kim M, Lai D, Poerio A, Ju Y, et al. Synergistic effect of CNTF and GDNF on directed neurite growth in chick embryo dorsal root ganglia. PLoS ONE. 2020;15:e0240235 pubmed publisher
Men Y, Ye L, Risgaard R, Promes V, Zhao X, Paukert M, et al. Astroglial FMRP deficiency cell-autonomously up-regulates miR-128 and disrupts developmental astroglial mGluR5 signaling. Proc Natl Acad Sci U S A. 2020;117:25092-25103 pubmed publisher
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Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, et al. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun. 2019;7:208 pubmed publisher
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Sieving P, Caruso R, Tao W, Coleman H, Thompson D, Fullmer K, et al. Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants. Proc Natl Acad Sci U S A. 2006;103:3896-901 pubmed
He W, Gong K, Smith D, Ip N. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett. 2005;579:4317-23 pubmed
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image
image 1 :
R&D Systems 257-NT-010 image 1
Recombinant Human CNTF Protein Bioactivity
product information
brand :
R&D Systems, a Bio-Techne brand
catalog number base :
257-NT
SKU :
257-NT-010
product name :
Recombinant Human CNTF Protein
description :
The Recombinant Human CNTF Protein from R&D Systems is derived from E. coli. The Recombinant Human CNTF Protein has been validated for the following applications: Bioactivity.
target :
CNTF
unit size :
10 ug (also 50 ug)
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 µm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.
observed molecular weight :
23-26 kDa, reducing conditions
theoretical molecular weight :
22.8 kDa
gene symbol :
CNTF
details of functionality :
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 0.03-0.18 µg/mL. The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR ) .
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P26441.1
applications :
Bioactivity
product keywords :
257-NT, rhCNTF, hCNTF,, 257NT, cytokinegrowthfact, cytokine, cytokines
source :
E. coli-derived Recombinant Human CNTF Protein
USD :
249
USD 2025 :
252 USD
alt names :
ciliary neurotrophic factor, HCNTF
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.