protein

Recombinant Human SCF GMP Protein, CF

255B-GMP-050

50 ug (also 1 mg)

255B-GMP-050

Recombinant Human SCF GMP Protein, CF

50 ug (also 1 mg)

The Recombinant Human SCF GMP Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human SCF GMP Protein, CF has been validated for the following applications: Bioactivity.

SCF/c-kit Ligand

Proteins and Enzymes

Lyophilized from a 0.2 ╡m filtered solution in PBS.

Unconjugated

97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining. The result from mass spectrometry confirms the major peak corresponds to SCF/c-kit ligand. See the CofA for additional information

Human

17-18 kDa, reducing conditions

19 kDa

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 1-5 ng/mL. The specific activity of recombinant human SCF is 1.0 x 106 units/mg, which is calibrated against the human SCF reference standard (NIBSC code: 91/682).

<0.10 EU per 1 ╡g of the protein by the LAL method.

Recombinant Human SCF GMP Protein Bioactivity

P21583.1

Bioactivity

E. coli -derived human SCF/c-kit Ligand protein Glu26-Ala189, with an N-terminal Met. Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.

E. coli

Animal-Free

Animal-Free

c-kit Ligand, DCUA, DFNA69, DKFZp686F2250, familial progressive hyperpigmentation 2, FPH2, FPHH, KIT ligand, Kitl, KITLG, KL-1, Mast cell growth factor, MGF, MGFSHEP7, SCFStem cell factor, SFc-Kit ligand, SHEP7, SLF, steel factor

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 ░C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, <= -20 ░C under sterile conditions after reconstitution.