This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-27 Protein
catalog :
2526-IL
quantity :
10 ug
price :
329 USD
citations: 33
Reference
Otterdal K, Berg A, Michelsen A, Patel S, Gregersen I, Sagen E, et al. Plasma levels of interleukin 27 in falciparum malaria is increased independently of co-infection with HIV: potential immune-regulatory role during malaria. BMC Infect Dis. 2020;20:65 pubmed publisher
Hu X, Goswami S, Qiu J, Chen Q, Laverdure S, Sherman B, et al. Profiles of Long Non-Coding RNAs and mRNA Expression in Human Macrophages Regulated by Interleukin-27. Int J Mol Sci. 2019;20: pubmed publisher
Yamasoba D, Sato K, Ichinose T, Imamura T, Koepke L, Joas S, et al. N4BP1 restricts HIV-1 and its inactivation by MALT1 promotes viral reactivation. Nat Microbiol. 2019;: pubmed publisher
Zhang Q, Cunha A, Li S, Hao Q, Kainz V, Huang Q, et al. IL-27 regulates HIF-1α-mediated VEGFA response in macrophages of diabetic retinopathy patients and healthy individuals. Cytokine. 2019;113:238-247 pubmed publisher
Petes C, Mintsopoulos V, Finnen R, Banfield B, Gee K. The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages. J Biol Chem. 2018;293:17631-17645 pubmed publisher
Petes C, Odoardi N, Plater S, Martin N, Gee K. IL-27 amplifies cytokine responses to Gram-negative bacterial products and Salmonella typhimurium infection. Sci Rep. 2018;8:13704 pubmed publisher
Rolvering C, Zimmer A, Ginolhac A, Margue C, Kirchmeyer M, Servais F, et al. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies. J Leukoc Biol. 2018;104:969-985 pubmed publisher
Patel M, Shen Z, Rossoll R, Wira C. IL-27 Expression and Responsiveness in Human Uterine Epithelial Cells and Fibroblasts In Vitro and the Role of Estradiol. J Interferon Cytokine Res. 2018;38:101-110 pubmed publisher
Gregersen I, Sandanger Ø, Askevold E, Sagen E, Yang K, Holm S, et al. Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation. PLoS ONE. 2017;12:e0188387 pubmed publisher
Zhang Y, Ma C, Lawrence M, Break T, O Connell M, Lyons J, et al. PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients. J Exp Med. 2017;214:2523-2533 pubmed publisher
Richard J, Prévost J, von Bredow B, Ding S, Brassard N, Medjahed H, et al. BST-2 Expression Modulates Small CD4-Mimetic Sensitization of HIV-1-Infected Cells to Antibody-Dependent Cellular Cytotoxicity. J Virol. 2017;91: pubmed publisher
Dixon K, O Flynn J, Klar Mohamad N, Daha M, van Kooten C. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?. Eur J Immunol. 2017;47:470-480 pubmed publisher
Rolvering C, Zimmer A, Kozar I, Hermanns H, Letellier E, Vallar L, et al. Crosstalk between different family members: IL27 recapitulates IFN? responses in HCC cells, but is inhibited by IL6-type cytokines. Biochim Biophys Acta Mol Cell Res. 2017;1864:516-526 pubmed publisher
Carbotti G, Barisione G, Airoldi I, Mezzanzanica D, Bagnoli M, Ferrero S, et al. IL-27 induces the expression of IDO and PD-L1 in human cancer cells. Oncotarget. 2015;6:43267-80 pubmed publisher
Kudo H, Wang Z, Jinnin M, Nakayama W, Inoue K, Honda N, et al. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin. J Immunol. 2015;195:3565-73 pubmed publisher
Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, et al. Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway. J Biol Chem. 2015;290:19307-18 pubmed publisher
Teles R, Kelly Scumpia K, Sarno E, Rea T, Ochoa M, Cheng G, et al. IL-27 Suppresses Antimicrobial Activity in Human Leprosy. J Invest Dermatol. 2015;135:2410-2417 pubmed publisher
Morandi F, Airoldi I, Pistoia V. IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-? release by autologous NK cells. J Immunol Res. 2014;2014:938561 pubmed publisher
Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol. 2014;192:2634-42 pubmed publisher
Oliveira W, Ribeiro L, Schrieffer A, Machado P, Carvalho E, Bacellar O. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of human tegumentary leishmaniasis. Cytokine. 2014;66:127-32 pubmed publisher
Cao Y, Zhang R, Zhang W, Zhu C, Yu Y, Song Y, et al. IL-27, a cytokine, and IFN-?1, a type III IFN, are coordinated to regulate virus replication through type I IFN. J Immunol. 2014;192:691-703 pubmed publisher
Tzeng T, Tsay H, Chang L, Hsu C, Lai T, Huang F, et al. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid. J Biomed Sci. 2013;20:90 pubmed publisher
Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, et al. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer. J Exp Clin Cancer Res. 2013;32:97 pubmed publisher
Chen Q, Swaminathan S, Yang D, Dai L, Sui H, Yang J, et al. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway. PLoS ONE. 2013;8:e59194 pubmed publisher
Fitzgerald D, Fonseca Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, et al. Independent and interdependent immunoregulatory effects of IL-27, IFN-?, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis. J Immunol. 2013;190:3225-34 pubmed publisher
Perona Wright G, Kohlmeier J, Bassity E, Freitas T, Mohrs K, Cookenham T, et al. Persistent loss of IL-27 responsiveness in CD8+ memory T cells abrogates IL-10 expression in a recall response. Proc Natl Acad Sci U S A. 2012;109:18535-40 pubmed publisher
Guzzo C, Hopman W, Che Mat N, Wobeser W, Gee K. IL-27-induced gene expression is downregulated in HIV-infected subjects. PLoS ONE. 2012;7:e45706 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Morandi F, Prigione I, Airoldi I. Human TCR??+ T cells represent a novel target for IL-27 activity. Eur J Immunol. 2012;42:1547-52 pubmed publisher
Liu L, Cao Z, Chen J, Li R, Cao Y, Zhu C, et al. Influenza A virus induces interleukin-27 through cyclooxygenase-2 and protein kinase A signaling. J Biol Chem. 2012;287:11899-910 pubmed publisher
Cocco C, Di Carlo E, Zupo S, Canale S, Zorzoli A, Ribatti D, et al. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo. Leukemia. 2012;26:1365-74 pubmed publisher
Kalliolias G, Gordon R, Ivashkiv L. Suppression of TNF-? and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27. J Immunol. 2010;185:7047-56 pubmed publisher
Cocco C, Giuliani N, Di Carlo E, Ognio E, Storti P, Abeltino M, et al. Interleukin-27 acts as multifunctional antitumor agent in multiple myeloma. Clin Cancer Res. 2010;16:4188-97 pubmed publisher
product information
brand :
R&D Systems
master code :
2526-IL
SKU :
2526-IL-010
product name :
Recombinant Human IL-27 Protein
description :
The Recombinant Human IL-27 Protein from R&D Systems is derived from NS0. The Recombinant Human IL-27 Protein has been validated for the following applications: Bioactivity.
target :
IL-27
category :
Proteins and Enzymes
unit size :
10 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
60 kDa, reducing conditions
theoretical molecular weight :
50 kDa
gene symbol :
IL27
details of functionality :
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al . (2009) Hepatology 50 :585. The ED50 for this effect is 1-8 ng/mL.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
catalog number base :
2526-IL
accessionNumbers :
Q14213.2 (EBI3) AAM34498 (p28)
applications :
Bioactivity
source :
Mouse myeloma cell line, NS0-derived human IL-27 protein Human IL-27 EBI-3 (Arg21-Lys229) Accession # Q14213.2 GSGSSRGGSGSGGSGGGGSKL Human IL-27 p28 (Phe29-Pro243) Accession # AAM34498 6-His tag N-terminus C-terminus
2020 USD :
329
2021 USD :
329 USD
alt names :
IL27, IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL27A, IL-27-A, IL27-A, IL27p28, IL30, interleukin 27, interleukin 30, interleukin-27 subunit alpha, MGC71873, p28IL-27A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.