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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-27 Protein, CF
catalog :
2526-IL-010/CF
quantity :
10 ug
price :
400 USD
more info or order :
citations: 44
Reference
Gringhuis S, Kaptein T, Remmerswaal E, Drewniak A, Wevers B, Theelen B, et al. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs. Nat Immunol. 2022;23:1735-1748 pubmed publisher
Lema xee tre F, Farzam Kia N, Carmena Moratalla A, Carpentier Solorio Y, Clénet M, Tastet O, et al. IL-27 shapes the immune properties of human astrocytes and their impact on encountered human T lymphocytes. J Neuroinflammation. 2022;19:212 pubmed publisher
Nagel S, Meyer C, Eberth S, Haake J, Pommerenke C. Downregulation of STAT3 in Epstein-Barr Virus-Positive Hodgkin Lymphoma. Biomedicines. 2022;10: pubmed publisher
Lindkvist M, Zegeye M, Greneg xe5 rd M, Ljungberg L. Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells. Int J Mol Sci. 2022;23: pubmed publisher
Otterdal K, Berg A, Michelsen A, Yndestad A, Patel S, Gregersen I, et al. IL-18 and IL-18 binding protein are related to disease severity and parasitemia during falciparum malaria. BMC Infect Dis. 2021;21:1073 pubmed publisher
Carbotti G, Dozin B, Martini S, Giordano C, Scordamaglia F, Croce M, et al. IL-27 Mediates PD-L1 Expression and Release by Human Mesothelioma Cells. Cancers (Basel). 2021;13: pubmed publisher
Natale M, Minning T, Albareda M, Castro Eiro M, Alvarez M, Lococo B, et al. Immune exhaustion in chronic Chagas disease: Pro-inflammatory and immunomodulatory action of IL-27 in vitro. PLoS Negl Trop Dis. 2021;15:e0009473 pubmed publisher
Chen M, Huang X, Wang H, Hegner C, Liu Y, Shang J, et al. CAR directs T cell adaptation to bile acids in the small intestine. Nature. 2021;593:147-151 pubmed publisher
Imamichi T, Goswami S, Hu X, Laverdure S, Yang J, Qiu J, et al. MicroRNA Profiles in Monocyte-Derived Macrophages Generated by Interleukin-27 and Human Serum: Identification of a Novel HIV-Inhibiting and Autophagy-Inducing MicroRNA. Int J Mol Sci. 2021;22: pubmed publisher
Srinivasachar Badarinarayan S, Shcherbakova I, Langer S, Koepke L, Preising A, Hotter D, et al. HIV-1 infection activates endogenous retroviral promoters regulating antiviral gene expression. Nucleic Acids Res. 2020;48:10890-10908 pubmed publisher
Carbotti G, Petretto A, Naschberger E, Sturzl M, Martini S, Mingari M, et al. Cytokine-Induced Guanylate Binding Protein 1 (GBP1) Release from Human Ovarian Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
Otterdal K, Berg A, Michelsen A, Patel S, Gregersen I, Sagen E, et al. Plasma levels of interleukin 27 in falciparum malaria is increased independently of co-infection with HIV: potential immune-regulatory role during malaria. BMC Infect Dis. 2020;20:65 pubmed publisher
Hu X, Goswami S, Qiu J, Chen Q, Laverdure S, Sherman B, et al. Profiles of Long Non-Coding RNAs and mRNA Expression in Human Macrophages Regulated by Interleukin-27. Int J Mol Sci. 2019;20: pubmed publisher
Yamasoba D, Sato K, Ichinose T, Imamura T, Koepke L, Joas S, et al. N4BP1 restricts HIV-1 and its inactivation by MALT1 promotes viral reactivation. Nat Microbiol. 2019;: pubmed publisher
Petes C, Mintsopoulos V, Finnen R, Banfield B, Gee K. The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages. J Biol Chem. 2018;293:17631-17645 pubmed publisher
Petes C, Odoardi N, Plater S, Martin N, Gee K. IL-27 amplifies cytokine responses to Gram-negative bacterial products and Salmonella typhimurium infection. Sci Rep. 2018;8:13704 pubmed publisher
Rolvering C, Zimmer A, Ginolhac A, Margue C, Kirchmeyer M, Servais F, et al. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies. J Leukoc Biol. 2018;104:969-985 pubmed publisher
Zhang Q, Cunha A, Li S, Hao Q, Kainz V, Huang Q, et al. IL-27 regulates HIF-1α-mediated VEGFA response in macrophages of diabetic retinopathy patients and healthy individuals. Cytokine. 2019;113:238-247 pubmed publisher
Patel M, Shen Z, Rossoll R, Wira C. IL-27 Expression and Responsiveness in Human Uterine Epithelial Cells and Fibroblasts In Vitro and the Role of Estradiol. J Interferon Cytokine Res. 2018;38:101-110 pubmed publisher
Gregersen I, Sandanger Ø, Askevold E, Sagen E, Yang K, Holm S, et al. Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation. PLoS ONE. 2017;12:e0188387 pubmed publisher
Zhang Y, Ma C, Lawrence M, Break T, O Connell M, Lyons J, et al. PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients. J Exp Med. 2017;214:2523-2533 pubmed publisher
Richard J, Prévost J, von Bredow B, Ding S, Brassard N, Medjahed H, et al. BST-2 Expression Modulates Small CD4-Mimetic Sensitization of HIV-1-Infected Cells to Antibody-Dependent Cellular Cytotoxicity. J Virol. 2017;91: pubmed publisher
Dixon K, O Flynn J, Klar Mohamad N, Daha M, van Kooten C. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?. Eur J Immunol. 2017;47:470-480 pubmed publisher
Rolvering C, Zimmer A, Kozar I, Hermanns H, Letellier E, Vallar L, et al. Crosstalk between different family members: IL27 recapitulates IFN? responses in HCC cells, but is inhibited by IL6-type cytokines. Biochim Biophys Acta Mol Cell Res. 2017;1864:516-526 pubmed publisher
Kudo H, Wang Z, Jinnin M, Nakayama W, Inoue K, Honda N, et al. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin. J Immunol. 2015;195:3565-73 pubmed publisher
Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, et al. Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway. J Biol Chem. 2015;290:19307-18 pubmed publisher
Teles R, Kelly Scumpia K, Sarno E, Rea T, Ochoa M, Cheng G, et al. IL-27 Suppresses Antimicrobial Activity in Human Leprosy. J Invest Dermatol. 2015;135:2410-2417 pubmed publisher
Xie M, Mustovich A, Jiang Y, Trudeau J, Ray A, Ray P, et al. IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signaling. J Allergy Clin Immunol. 2015;135:386-94 pubmed publisher
Morandi F, Airoldi I, Pistoia V. IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-? release by autologous NK cells. J Immunol Res. 2014;2014:938561 pubmed publisher
Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol. 2014;192:2634-42 pubmed publisher
Oliveira W, Ribeiro L, Schrieffer A, Machado P, Carvalho E, Bacellar O. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of human tegumentary leishmaniasis. Cytokine. 2014;66:127-32 pubmed publisher
Cao Y, Zhang R, Zhang W, Zhu C, Yu Y, Song Y, et al. IL-27, a cytokine, and IFN-?1, a type III IFN, are coordinated to regulate virus replication through type I IFN. J Immunol. 2014;192:691-703 pubmed publisher
Tzeng T, Tsay H, Chang L, Hsu C, Lai T, Huang F, et al. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid. J Biomed Sci. 2013;20:90 pubmed publisher
Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, et al. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer. J Exp Clin Cancer Res. 2013;32:97 pubmed publisher
Chen Q, Swaminathan S, Yang D, Dai L, Sui H, Yang J, et al. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway. PLoS ONE. 2013;8:e59194 pubmed publisher
Fitzgerald D, Fonseca Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, et al. Independent and interdependent immunoregulatory effects of IL-27, IFN-?, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis. J Immunol. 2013;190:3225-34 pubmed publisher
Perona Wright G, Kohlmeier J, Bassity E, Freitas T, Mohrs K, Cookenham T, et al. Persistent loss of IL-27 responsiveness in CD8+ memory T cells abrogates IL-10 expression in a recall response. Proc Natl Acad Sci U S A. 2012;109:18535-40 pubmed publisher
Guzzo C, Hopman W, Che Mat N, Wobeser W, Gee K. IL-27-induced gene expression is downregulated in HIV-infected subjects. PLoS ONE. 2012;7:e45706 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Morandi F, Prigione I, Airoldi I. Human TCR??+ T cells represent a novel target for IL-27 activity. Eur J Immunol. 2012;42:1547-52 pubmed publisher
Liu L, Cao Z, Chen J, Li R, Cao Y, Zhu C, et al. Influenza A virus induces interleukin-27 through cyclooxygenase-2 and protein kinase A signaling. J Biol Chem. 2012;287:11899-910 pubmed publisher
Cocco C, Di Carlo E, Zupo S, Canale S, Zorzoli A, Ribatti D, et al. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo. Leukemia. 2012;26:1365-74 pubmed publisher
Kalliolias G, Gordon R, Ivashkiv L. Suppression of TNF-? and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27. J Immunol. 2010;185:7047-56 pubmed publisher
Cocco C, Giuliani N, Di Carlo E, Ognio E, Storti P, Abeltino M, et al. Interleukin-27 acts as multifunctional antitumor agent in multiple myeloma. Clin Cancer Res. 2010;16:4188-97 pubmed publisher
product information
brand :
R&D Systems, a Bio-Techne brand
catalog number base :
2526-IL/CF
SKU :
2526-IL-010/CF
product name :
Recombinant Human IL-27 Protein, CF
description :
The Recombinant Human IL-27 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human IL-27 Protein, CF has been validated for the following applications: Bioactivity.
target :
IL-27
unit size :
10 ug
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl, EDTA and CHAPS with Trehalose.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
observed molecular weight :
60 kDa, reducing conditions
theoretical molecular weight :
50 kDa
gene symbol :
IL27
details of functionality :
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al . (2009) Hepatology 50 :585. The ED50 for this effect is 1-8 ng/mL.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q14213.2 (EBI3) AAM34498 (p28)
applications :
Bioactivity
product keywords :
2526-IL, rhIL-27, hIL-27, carrier-free, carrier free, 2526IL/CF, cytokinegrowthfact, IL27, IL 27, Interleukin 27, Interleukin-27, cytokine, cytokines
source :
NS0-derived Recombinant Human IL-27 Protein, CF
USD :
396
USD 2025 :
400 USD
alt names :
IL27, IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL27A, IL-27-A, IL27-A, IL27p28, IL30, interleukin 27, interleukin 30, interleukin-27 subunit alpha, MGC71873, p28IL-27A
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.