Recombinant Human Insulysin/IDE Protein, CF | R&D Systems 2496-ZN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Insulysin/IDE Protein, CF

catalog :

2496-ZN

quantity :

10 ug

price :

369 USD

citations: 7

Reference
Sbardella D, Tundo G, Coletta A, Marcoux J, Koufogeorgou E, Ciaccio C, et al. The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S. Cell Mol Life Sci. 2018;75:3441-3456 pubmed publisher
Portelius E, Mattsson N, Pannee J, Zetterberg H, GisslÃ©n M, Vanderstichele H, et al. Ex vivo 18O-labeling mass spectrometry identifies a peripheral amyloid Î² clearance pathway. Mol Neurodegener. 2017;12:18 pubmed publisher
Smith A, Rajapakse N, Kleifeld O, Lomonte B, Sikanyika N, Spicer A, et al. N-terminal domain of Bothrops asper Myotoxin II Enhances the Activity of Endothelin Converting Enzyme-1 and Neprilysin. Sci Rep. 2016;6:22413 pubmed publisher
Humpel C. Organotypic vibrosections from whole brain adult Alzheimer mice (overexpressing amyloid-precursor-protein with the Swedish-Dutch-Iowa mutations) as a model to study clearance of beta-amyloid plaques. Front Aging Neurosci. 2015;7:47 pubmed publisher
Liu Z, Zhu H, Fang G, Walsh K, Mwamburi M, Wolozin B, et al. Characterization of insulin degrading enzyme and other amyloid-? degrading proteases in human serum: a role in Alzheimer's disease?. J Alzheimers Dis. 2012;29:329-40 pubmed publisher
Lan X, Xu J, Kiyota T, Peng H, Zheng J, Ikezu T. HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes. J Immunol. 2011;186:6925-32 pubmed publisher
Miners J, Verbeek M, Rikkert M, Kehoe P, Love S. Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid. J Neurosci Methods. 2008;167:229-36 pubmed

product information

brand :

R&D Systems

master code :

2496-ZN

SKU :

2496-ZN-010

product name :

Recombinant Human Insulysin/IDE Protein, CF

description :

The Recombinant Human Insulysin/IDE Protein, CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human Insulysin/IDE Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Insulysin/IDE

category :

Proteins and Enzymes

unit size :

10 ug

buffer :

Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij-35 and Glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

105 kDa, reducing conditions

theoretical molecular weight :

114 kDa

gene symbol :

IDE

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005 ). The specific activity is 1,000 pmol/min/µg, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

2496-ZN

accessionNumbers :

P14735

applications :

Enzyme Activity

source :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived human Insulysin/IDE protein Met42-Leu1019, with an N-terminal Met and 7-His tag

2020 USD :

349

2021 USD :

369 USD

alt names :

Abeta-degrading protease, EC 3.4.24, EC 3.4.24.56, FLJ35968, IDE, INSDEGM, Insulin protease, insulinase, insulin-degrading enzyme, Insulysin

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.