Recombinant Human BDNF GMP, CF | R&D Systems 248-GMP-005 product information

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product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human BDNF GMP, CF

catalog :

248-GMP-005

quantity :

5 ug (also 25 ug)

product information

brand :

R&D Systems

MasterCode :

248-GMP

SKU :

248-GMP-005

product name :

Recombinant Human BDNF GMP, CF

unit sizes :

5 ug (also 25 ug)

description :

The Recombinant Human BDNF GMP, CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human BDNF GMP, CF has been validated for the following applications: Bioactivity.

target :

BDNF

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 µm filtered solution in Sodium Citrate and NaCl.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE with silver staining, under reducing conditions.

species :

Human, Mouse, Rat, Canine, Equine

observed molecular weight :

13-14 kDa, reducing conditions

theoretical molecular weight :

13.5 kDa

gene symbol :

BDNF

details of functionality :

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of Recombinant Human BDNF is 3.0 x 105 units/mg, which is calibrated against the human BDNF WHO Standard (NIBSC code: 96/534). Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK ) is coated at 1 µg/mL, recombinant human BDNF binds with an apparent Kd 1 nM.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P23560

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived BDNF protein His129-Arg247 Accession # P23560 100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined. Manufactured and tested under cGMP guidelines.

source short :

Sf 21 (baculovirus)

extended description :

100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.

product details :

100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.

alt names :

abrineurin, ANON2, brain-derived neurotrophic factor, BULN2, MGC34632, neurotrophin

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.