Recombinant Human LAP (TGF-beta 1) Protein | R&D Systems 246-LP-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human LAP (TGF-beta 1) Protein

catalog :

246-LP-025

quantity :

25 ug

price :

480 USD

citations: 29

Reference
Mendoza M, Ballesteros A, Rendon Correa E, Tonk R, Warren J, Snow A, et al. Modulation of galectin-9 mediated responses in monocytes and T-cells by pregnancy-specific glycoprotein 1. J Biol Chem. 2024;300:107638 pubmed publisher
Ishiyama S, Hayatsu M, Toriumi T, Tsuda H, Watanabe K, Kasai H, et al. Assessing the combined impact of fatty liver-induced TGF-β1 and LPS-activated macrophages in fibrosis through a novel 3D serial section methodology. Sci Rep. 2024;14:11404 pubmed publisher
Sofroniou M, Lemmon C. Differential regulation of fibronectin expression and fibrillogenesis by autocrine TGF-β1 signaling in malignant and benign mammary epithelial cells. Int J Biochem Cell Biol. 2023;165:106478 pubmed publisher
Roy A, Shi L, Chang A, Dong X, Fernandez A, Kraft J, et al. De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8. Nat Commun. 2023;14:5660 pubmed publisher
Haudenschild A, Sherlock B, Zhou X, Sheaff C, Hu J, Leach J, et al. Non-destructive, continuous monitoring of biochemical, mechanical, and structural maturation in engineered tissue. Sci Rep. 2022;12:16227 pubmed publisher
Wu W, Huang X, You Y, Xue L, Wang X, Meng X, et al. Latent TGF-β1 protects against diabetic kidney disease via Arkadia/Smad7 signaling. Int J Biol Sci. 2021;17:3583-3594 pubmed publisher
Zhou M, Niu J, Wang J, Gao H, Shahbaz M, Niu Z, et al. Integrin αvβ8 serves as a Novel Marker of Poor Prognosis in Colon Carcinoma and Regulates Cell Invasiveness through the Activation of TGF-β1. J Cancer. 2020;11:3803-3815 pubmed publisher
Basta J, Robbins L, Stout L, Prinsen M, Griggs D, Rauchman M. Pharmacologic inhibition of RGD-binding integrins ameliorates fibrosis and improves function following kidney injury. Physiol Rep. 2020;8:e14329 pubmed publisher
Briggs D, Langford Smith A, Birchenough H, Jowitt T, Kielty C, Enghild J, et al. Inter-α-inhibitor heavy chain-1 has an integrin-like 3D structure mediating immune regulatory activities and matrix stabilization during ovulation. J Biol Chem. 2020;295:5278-5291 pubmed publisher
Tsui P, Higazi D, Wu Y, Dunmore R, Solier E, Kasali T, et al. The TGF-? inhibitory activity of antibody 37E1B5 depends on its H-CDR2 glycan. MAbs. 2017;9:104-113 pubmed publisher
Jones K, Ballesteros A, Mentink Kane M, Warren J, Rattila S, Malech H, et al. PSG9 Stimulates Increase in FoxP3+ Regulatory T-Cells through the TGF-?1 Pathway. PLoS ONE. 2016;11:e0158050 pubmed publisher
Fan M, Zhu Q, Li H, Ra H, Majumdar S, Gulick D, et al. Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice. J Biol Chem. 2016;291:8070-89 pubmed publisher
Turner C, Badu Nkansah K, Crowley D, van der Flier A, Hynes R. α5 and αv integrins cooperate to regulate vascular smooth muscle and neural crest functions in vivo. Development. 2015;142:797-808 pubmed publisher
Fedele C, Singh A, Zerlanko B, Iozzo R, Languino L. The Î±vÎ²6 integrin is transferred intercellularly via exosomes. J Biol Chem. 2015;290:4545-51 pubmed publisher
Ballesteros A, Mentink Kane M, Warren J, Kaplan G, Dveksler G. Induction and activation of latent transforming growth factor-Î²1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). J Biol Chem. 2015;290:4422-31 pubmed publisher
Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S. Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-β in early immune response. PLoS Negl Trop Dis. 2014;8:e2995 pubmed publisher
Dutta A, Li J, Lu H, Akech J, Pratap J, Wang T, et al. Integrin ?v?6 promotes an osteolytic program in cancer cells by upregulating MMP2. Cancer Res. 2014;74:1598-608 pubmed publisher
Mele V, Muraro M, Calabrese D, Pfaff D, Amatruda N, Amicarella F, et al. Mesenchymal stromal cells induce epithelial-to-mesenchymal transition in human colorectal cancer cells through the expression of surface-bound TGF-?. Int J Cancer. 2014;134:2583-94 pubmed publisher
Willet J, Pichitsiri W, Jenkinson S, Brain J, Wood K, Alhasan A, et al. Kidney transplantation: analysis of the expression and T cell-mediated activation of latent TGF-β. J Leukoc Biol. 2013;93:471-8 pubmed publisher
Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, et al. Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Cell. 2011;147:1146-58 pubmed publisher
Oida T, Weiner H. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß. J Immunol. 2010;185:3529-35 pubmed publisher
Tran D, Glass D, Uzel G, Darnell D, Spalding C, Holland S, et al. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. J Immunol. 2009;182:2929-38 pubmed publisher
Savage N, De Boer T, Walburg K, Joosten S, van Meijgaarden K, Geluk A, et al. Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1. J Immunol. 2008;181:2220-6 pubmed
Rojas A, Meherem S, Kim Y, Washington M, Willis J, Markowitz S, et al. The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer. Int J Cancer. 2008;123:14-21 pubmed publisher
Gressner O, Lahme B, Siluschek M, Rehbein K, Herrmann J, Weiskirchen R, et al. Activation of TGF-beta within cultured hepatocytes and in liver injury leads to intracrine signaling with expression of connective tissue growth factor. J Cell Mol Med. 2008;12:2717-30 pubmed publisher
Van Aarsen L, Leone D, Ho S, Dolinski B, McCoon P, Lepage D, et al. Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism. Cancer Res. 2008;68:561-70 pubmed publisher
Godfrey W, Spoden D, Ge Y, Baker S, Liu B, Levine B, et al. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood. 2005;105:750-8 pubmed
Nakamura K, Kitani A, Fuss I, Pedersen A, Harada N, Nawata H, et al. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice. J Immunol. 2004;172:834-42 pubmed
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product information

master code :

246-LP

SKU :

246-LP-025

product name :

Recombinant Human LAP (TGF-beta 1) Protein

unit size :

25 ug

description :

The Recombinant Human LAP (TGF-beta 1) Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human LAP (TGF-beta 1) Protein has been validated for the following applications: Bioactivity.

target :

LAP (TGF-beta 1)

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

28-36 kDa, reducing 60-70 kDa, non-reducing conditions (variably glycosylated)

theoretical molecular weight :

27 kDa (monomer)

gene symbol :

TGFB1

details of functionality :

Measured by its ability to inhibit TGF┐▀1 activity on HT-2 mouse T cells. Tsang,áM. etáal . (1995) Cytokine 7 :389. The ED50 for this effect is 50-300 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF┐▀1 (Catalog # 240-B ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P01137.2

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived human LAP (TGF-beta 1) protein Leu30-Arg278 (Cys33Ser)

source short :

Sf 21 (baculovirus)

USD :

480 USD

alt names :

CED, DPD1, LAP, LAP (TGFbeta 1), TGFB, TGFB1, TGFbeta, transforming growth factor beta 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.