Recombinant Mouse Dkk-2 Protein | R&D Systems 2435-DKB-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Dkk-2 Protein

catalog :

2435-DKB-010

quantity :

10 ug

price :

541 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Corliss B, Ray H, Mathews C, Fitzgerald K, Doty R, Smolko C, et al. Myh11 Lineage Corneal Endothelial Cells and ASCs Populate Corneal Endothelium. Invest Ophthalmol Vis Sci. 2019;60:5095-5103 pubmed publisher

product information

master code :

2435-DKB

SKU :

2435-DKB-010

product name :

Recombinant Mouse Dkk-2 Protein

unit size :

10 ug

description :

The Recombinant Mouse Dkk-2 Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Mouse Dkk-2 Protein has been validated for the following applications: Bioactivity.

target :

Dkk-2

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Mouse

observed molecular weight :

29-35 kDa, reducing conditions

theoretical molecular weight :

27 kDa

details of functionality :

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells. Recombinant Mouse Dkk-2 (Catalog # 2435-DKB) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a Recombinant Mouse Wnt-3a (Catalog # 1324-WN ) . The ED50 for this effect is 100-600 ng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Mouse Dkk-2 Protein Bioactivity

accessionNumbers :

Q9QYZ8.1

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived mouse Dkk-2 protein Ser26-Ile259, with Leu35Pro substitution and a C-terminal 10-His tag

source short :

Sf 21 (baculovirus)

USD :

541 USD

alt names :

dickkopf (Xenopus laevis) homolog 2, dickkopf 2, dickkopf homolog 2 (Xenopus laevis), dickkopf related protein-2, Dickkopf-2, dickkopf-related protein 2, Dkk2, DKK-2, hDkk-2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage