Recombinant Human B7-H3 (4Ig)/B7-H3b Protein | R&D Systems 2318-B3-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human B7-H3 (4Ig)/B7-H3b Protein

catalog :

2318-B3-050

quantity :

50 ug

price :

468 USD

citations: 4

Reference
Li D, Wang R, Liang T, Ren H, Park C, Tai C, et al. Camel nanobody-based B7-H3 CAR-T cells show high efficacy against large solid tumours. Nat Commun. 2023;14:5920 pubmed publisher
Seo Y, Lee J, Ryu H, Kim E, Kim S, Jeong J, et al. Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis. Sci Rep. 2023;13:14242 pubmed publisher
Li H, Yang Z, Yang X, Zhang F, Wang J, Wu Z, et al. LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma. Cell Death Dis. 2022;13:109 pubmed publisher
Yoon B, Chung Y, Yoo S, Kawara K, Kim J, Yoo I, et al. Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis. J Biol Chem. 2016;291:4048-57 pubmed publisher

product information

master code :

2318-B3

SKU :

2318-B3-050

product name :

Recombinant Human B7-H3 (4Ig)/B7-H3b Protein

unit size :

50 ug

description :

The Recombinant Human B7-H3 (4Ig)/B7-H3b Protein from R&D Systems is derived from NS0. The Recombinant Human B7-H3 (4Ig)/B7-H3b Protein has been validated for the following applications: Bioactivity.

target :

B7-H3

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

78-90 kDa, reducing conditions

theoretical molecular weight :

48 kDa

gene symbol :

CD276

details of functionality :

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and/or 10 ╡g/mL rhB7-H3 (4Ig)/B7-H3b. The presence of rhB7-H3 (4Ig)/B7-H3b at 10 ╡g/mL inhibited the anti-CD3 response by 30%. Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q5ZPR3

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human B7-H3 protein Gly27-Thr461, with a C-terminal 10-His tag

source short :

NS0

USD :

468 USD

alt names :

B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.