Recombinant Bovine TNF-alpha Protein | R&D Systems 2279-BT-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Bovine TNF-alpha Protein

catalog :

2279-BT-025

quantity :

25 ug

price :

373 USD

more info or order :

R&D Systems product webpage

citations: 13

Reference
Montgomery Song A, Ashraf S, Santerre P, Kandel R. Senescent response in inner annulus fibrosus cells in response to TNFα, H2O2, and TNFα-induced nucleus pulposus senescent secretome. PLoS ONE. 2024;19:e0280101 pubmed publisher
Chan B, Glogauer M, Wang Y, Wrana J, Chan K, Beier F, et al. Adseverin, an actin-binding protein, modulates hypertrophic chondrocyte differentiation and osteoarthritis progression. Sci Adv. 2023;9:eadf1130 pubmed publisher
Alizada A, Khyzha N, Wang L, Antounians L, Chen X, Khor M, et al. Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals. Nat Commun. 2021;12:567 pubmed publisher
Terabe K, Ohashi Y, Tsuchiya S, Ishizuka S, Knudson C, Knudson W. Chondroprotective effects of 4-methylumbelliferone and hyaluronan synthase-2 overexpression involve changes in chondrocyte energy metabolism. J Biol Chem. 2019;294:17799-17817 pubmed publisher
Ding L, Buckwalter J, Martin J. DAMPs Synergize with Cytokines or Fibronectin Fragment on Inducing Chondrolysis but Lose Effect When Acting Alone. Mediators Inflamm. 2017;2017:2642549 pubmed publisher
Hammer S, Beli E, Kady N, Wang Q, Wood K, Lydic T, et al. The Mechanism of Diabetic Retinopathy Pathogenesis Unifying Key Lipid Regulators, Sirtuin 1 and Liver X Receptor. EBioMedicine. 2017;22:181-190 pubmed publisher
Sakumoto R, Hayashi K, Fujii S, Kanahara H, Hosoe M, Furusawa T, et al. Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy. Int J Mol Sci. 2017;18: pubmed publisher
Ding Y, Floren M, Tan W. High-Throughput Screening of Vascular Endothelium-Destructive or Protective Microenvironments: Cooperative Actions of Extracellular Matrix Composition, Stiffness, and Structure. Adv Healthc Mater. 2017;6: pubmed publisher
Spicer L, SchÃ¼tz L, Williams J, Schreiber N, Evans J, Totty M, et al. G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications. Domest Anim Endocrinol. 2017;59:90-99 pubmed publisher
Yang S, Zhan Y, Zhou Y, Jiang Y, Zheng X, Yu L, et al. Interferon regulatory factor 3 is a key regulation factor for inducing the expression of SAMHD1 in antiviral innate immunity. Sci Rep. 2016;6:29665 pubmed publisher
Dooley L, Abdalmula A, Washington E, Kaufman C, Tudor E, Ghosh P, et al. Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis. PLoS ONE. 2015;10:e0124144 pubmed publisher
Tsoporis J, Izhar S, Parker T. Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha. J Biol Chem. 2008;283:30174-83 pubmed publisher
Sordillo L, Weaver J, Cao Y, Corl C, Sylte M, Mullarky I. Enhanced 15-HPETE production during oxidant stress induces apoptosis of endothelial cells. Prostaglandins Other Lipid Mediat. 2005;76:19-34 pubmed

product information

brand :

R&D Systems

master code :

2279-BT

SKU :

2279-BT-025

product name :

Recombinant Bovine TNF-alpha Protein

unit size :

25 ug

seo description :

The Recombinant Bovine TNF-alpha Protein from R&D Systems is derived from E. coli. The Recombinant Bovine TNF-alpha Protein has been validated for the following applications: Bioactivity.

target :

TNF-alpha

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

dilution :

Bioactivity

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Bovine

theoretical molecular weight :

17.6 kDa

gene symbol :

TNF

details of functionality :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycináD. Matthews,áN. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 0.025-0.1 ╡g/mL. When cytotoxicity is measured on the porcine PK-15 cells, the ED50 is 0.05-0.15 ng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q06599

applications :

Bioactivity

source :

E. coli-derived Recombinant Bovine TNF-alpha Protein

USD :

373 USD

alt names :

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage