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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-6R alpha Protein
catalog :
227-SR-025
quantity :
25 ug
price :
480 USD
more info or order :
citations: 45
Reference
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Talwar D, Messens J, Dick T. A role for annexin A2 in scaffolding the peroxiredoxin 2-STAT3 redox relay complex. Nat Commun. 2020;11:4512 pubmed publisher
Kang S, Tanaka T, Inoue H, Ono C, Hashimoto S, Kioi Y, et al. IL-6 trans-signaling induces plasminogen activator inhibitor-1 from vascular endothelial cells in cytokine release syndrome. Proc Natl Acad Sci U S A. 2020;117:22351-22356 pubmed publisher
Findeisen M, Allen T, Henstridge D, Kammoun H, Brandon A, Baggio L, et al. Treatment of type 2 diabetes with the designer cytokine IC7Fc. Nature. 2019;574:63-68 pubmed publisher
Aryappalli P, Shabbiri K, Masad R, Al Marri R, Haneefa S, Mohamed Y, et al. Inhibition of Tyrosine-Phosphorylated STAT3 in Human Breast and Lung Cancer Cells by Manuka Honey is Mediated by Selective Antagonism of the IL-6 Receptor. Int J Mol Sci. 2019;20: pubmed publisher
McGregor N, Murat M, Elango J, Poulton I, Walker E, Crimeen Irwin B, et al. IL-6 exhibits both cis- and trans-signaling in osteocytes and osteoblasts, but only trans-signaling promotes bone formation and osteoclastogenesis. J Biol Chem. 2019;294:7850-7863 pubmed publisher
Yamamoto S, Omori K, Mandai H, Nakayama M, Nakagawa S, Kobayashi H, et al. Fungal metabolite (+)-terrein suppresses IL-6/sIL-6R-induced CSF1 secretion by inhibiting JAK1 phosphorylation in human gingival fibroblasts. Heliyon. 2018;4:e00979 pubmed publisher
Tanaka Y, Sabharwal L, Ota M, Nakagawa I, Jiang J, Arima Y, et al. Presenilin 1 Regulates NF-?B Activation via Association with Breakpoint Cluster Region and Casein Kinase II. J Immunol. 2018;: pubmed publisher
Lyman M, Lieuw V, Richardson R, Timmer A, Stewart C, Granger S, et al. A bispecific antibody that targets IL-6 receptor and IL-17A for the potential therapy of patients with autoimmune and inflammatory diseases. J Biol Chem. 2018;293:9326-9334 pubmed publisher
Huang R, Sun Y, Ho C, Liu K, Tang Q, Xie Y, et al. IL-6 potentiates BMP-2-induced osteogenesis and adipogenesis via two different BMPR1A-mediated pathways. Cell Death Dis. 2018;9:144 pubmed publisher
Alsaffar H, Martino N, Garrett J, Adam A. Interleukin-6 promotes a sustained loss of endothelial barrier function via Janus kinase-mediated STAT3 phosphorylation and de novo protein synthesis. Am J Physiol Cell Physiol. 2018;314:C589-C602 pubmed publisher
Ravi A, Plumb J, Gaskell R, Mason S, Broome C, Booth G, et al. COPD monocytes demonstrate impaired migratory ability. Respir Res. 2017;18:90 pubmed publisher
Larsen J, Petersen C. SorLA in Interleukin-6 Signaling and Turnover. Mol Cell Biol. 2017;37: pubmed publisher
Wang J, Qiao C, Xiao H, Lin Z, Li Y, Zhang J, et al. Structure-based virtual screening and characterization of a novel IL-6 antagonistic compound from synthetic compound database. Drug Des Devel Ther. 2016;10:4091-4100 pubmed publisher
Bastidas Coral A, Bakker A, Zandieh Doulabi B, Kleverlaan C, Bravenboer N, Forouzanfar T, et al. Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells. Stem Cells Int. 2016;2016:1318256 pubmed publisher
König C, Morch E, Eitner A, Möller C, Turnquist B, Schaible H, et al. Involvement of Spinal IL-6 Trans-Signaling in the Induction of Hyperexcitability of Deep Dorsal Horn Neurons by Spinal Tumor Necrosis Factor-Alpha. J Neurosci. 2016;36:9782-91 pubmed publisher
Lee J, Kim J, Kim B, Han H, Kang S, Cho N. STAT3-induced WDR1 overexpression promotes breast cancer cell migration. Cell Signal. 2016;28:1753-60 pubmed publisher
Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, et al. A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors. Cancer Res. 2016;76:480-90 pubmed publisher
Bhardwaj R, Yester J, Singh S, Biswas D, Surace M, Waters M, et al. RelB/p50 complexes regulate cytokine-induced YKL-40 expression. J Immunol. 2015;194:2862-70 pubmed publisher
Isobe A, Sawada K, Kinose Y, Ohyagi Hara C, Nakatsuka E, Makino H, et al. Interleukin 6 receptor is an independent prognostic factor and a potential therapeutic target of ovarian cancer. PLoS ONE. 2015;10:e0118080 pubmed publisher
Sobotta M, Liou W, Stöcker S, Talwar D, Oehler M, Ruppert T, et al. Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling. Nat Chem Biol. 2015;11:64-70 pubmed publisher
Bu Y, Su F, Wang X, Gao H, Lei L, Chang N, et al. Protein tyrosine phosphatase PTPN9 regulates erythroid cell development through STAT3 dephosphorylation in zebrafish. J Cell Sci. 2014;127:2761-70 pubmed publisher
Shaw S, Bourne T, Meier C, Carrington B, Gelinas R, Henry A, et al. Discovery and characterization of olokizumab: a humanized antibody targeting interleukin-6 and neutralizing gp130-signaling. MAbs. 2014;6:774-82 pubmed publisher
O Reilly S, Ciechomska M, Cant R, van Laar J. Interleukin-6 (IL-6) trans signaling drives a STAT3-dependent pathway that leads to hyperactive transforming growth factor-? (TGF-?) signaling promoting SMAD3 activation and fibrosis via Gremlin protein. J Biol Chem. 2014;289:9952-60 pubmed publisher
McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, et al. NF-?B-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma. PLoS ONE. 2013;8:e78728 pubmed publisher
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Yoo H, Seo J, Yoo J. CTR9, a component of PAF complex, controls elongation block at the c-Fos locus via signal-dependent regulation of chromatin-bound NELF dissociation. PLoS ONE. 2013;8:e61055 pubmed publisher
Patel A, Zhu Y, Kuzhikandathil E, Banks W, Siegel A, Zalcman S. Soluble interleukin-6 receptor induces motor stereotypies and co-localizes with gp130 in regions linked to cortico-striato-thalamo-cortical circuits. PLoS ONE. 2012;7:e41623 pubmed publisher
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Umehara A, Nishioka M, Obata T, Ebina Y, Shiota H, Hashida S. A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus. Clin Biochem. 2009;42:1468-75 pubmed publisher
Yammani R, Long D, Loeser R. Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes. Arthritis Rheum. 2009;60:792-800 pubmed publisher
Ara T, Song L, Shimada H, Keshelava N, Russell H, Metelitsa L, et al. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res. 2009;69:329-37 pubmed publisher
Ahmed S, Marotte H, Kwan K, Ruth J, Campbell P, Rabquer B, et al. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production. Proc Natl Acad Sci U S A. 2008;105:14692-7 pubmed publisher
Ammit A, Moir L, Oliver B, Hughes J, Alkhouri H, Ge Q, et al. Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2007;292:L199-206 pubmed
Mozaffarian D, Rimm E, King I, Lawler R, McDonald G, Levy W. trans fatty acids and systemic inflammation in heart failure. Am J Clin Nutr. 2004;80:1521-5 pubmed
Bas S, Gauthier B, Spenato U, Stingelin S, Gabay C. CD14 is an acute-phase protein. J Immunol. 2004;172:4470-9 pubmed
Hurst S, McLoughlin R, Monslow J, Owens S, Morgan L, Fuller G, et al. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J Immunol. 2002;169:5244-51 pubmed
Erices A, Conget P, Rojas C, Minguell J. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Exp Cell Res. 2002;280:24-32 pubmed
product information
brand :
R&D Systems
master code :
227-SR
SKU :
227-SR-025
product name :
Recombinant Human IL-6R alpha Protein
unit size :
25 ug
seo description :
The Recombinant Human IL-6R alpha Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human IL-6R alpha Protein has been validated for the following applications: Bioactivity.
target :
IL-6R alpha
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
45-50 kDa, reducing conditions
theoretical molecular weight :
38 kDa
gene symbol :
IL6R
details of functionality :
Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. Saito, T. et al . (1991) J. Immunol. 147 :168. The ED50 for this effect is 5.0-15.0 ng/mL.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human IL-6R alpha Protein Bioactivity
accessionNumbers :
P08887.1
applications :
Bioactivity
source :
Sf 21 (baculovirus)-derived Recombinant Human IL-6R alpha Protein
USD :
480 USD
alt names :
CD126, gp80, IL-6 R alpha, IL6Q, IL6R, IL6R alpha, IL-6R-1, IL6RA, IL-6RA, IL6RQ, interleukin 6 receptor
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.