Recombinant Human PDGF-AB Protein, CF | R&D Systems 222-AB-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human PDGF-AB Protein, CF

catalog :

222-AB-010

quantity :

10 ug (also 50 ug)

price :

322 USD

more info or order :

R&D Systems product webpage

citations: 12

Reference
Marimoutou M, Patel V, Kim J, Schaible N, Alvarez J, Hughes J, et al. The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation. Toxics. 2024;12: pubmed publisher
Madsen S, Sand J, Juhl P, Karsdal M, Thudium C, Siebuhr A, et al. Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts' response to fibrotic growth factors. Sci Rep. 2023;13:9411 pubmed publisher
Hadjicharalambous M, Roux B, Csomor E, Feghali Bostwick C, Murray L, Clarke D, et al. Long intergenic non-coding RNAs regulate human lung fibroblast function: Implications for idiopathic pulmonary fibrosis. Sci Rep. 2019;9:6020 pubmed publisher
Mamer S, Chen S, Weddell J, Palasz A, Wittenkeller A, Kumar M, et al. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics. Sci Rep. 2017;7:16439 pubmed publisher
He H, Kuriyan A, Su C, Mahabole M, Zhang Y, Zhu Y, et al. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3. Sci Rep. 2017;7:43736 pubmed publisher
Hayes B, Riehle K, Shimizu Albergine M, Bauer R, Hudkins K, Johansson F, et al. Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis. PLoS ONE. 2014;9:e92925 pubmed publisher
Ding W, Knox T, Tschumper R, Wu W, Schwager S, Boysen J, et al. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood. 2010;116:2984-93 pubmed publisher
Ball S, Bayley C, Shuttleworth C, Kielty C. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells. Biochem J. 2010;427:29-40 pubmed publisher
Li R, Maminishkis A, Wang F, Miller S. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Invest Ophthalmol Vis Sci. 2007;48:5722-32 pubmed
Gupta S, Ramjaun A, Haiko P, Wang Y, Warne P, Nicke B, et al. Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice. Cell. 2007;129:957-68 pubmed
Rhodes K, Raivich G, Fawcett J. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience. 2006;140:87-100 pubmed
Nagineni C, Kutty V, Detrick B, Hooks J. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J Cell Physiol. 2005;203:35-43 pubmed

product information

master code :

222-AB

SKU :

222-AB-010

product name :

Recombinant Human PDGF-AB Protein, CF

unit size :

10 ug (also 50 ug)

description :

The Recombinant Human PDGF-AB Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human PDGF-AB Protein, CF has been validated for the following applications: Bioactivity.

target :

PDGF-AB

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Acetonitrile and TFA.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

17.5 kDa (A chain) 12.5 kDa (B chain), reducing conditions

theoretical molecular weight :

14.3 kDa (A chain) 12.3 kDa (B chain)

details of functionality :

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al . (1985) Methods Enzymol. 109 :749. The ED50 for this effect is 15-75 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002 ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P04085.1 (A chain) P01127.1 (B chain)

applications :

Bioactivity

source long :

E. coli -derived human PDGF-AB protein Human PDGF-A (Ser87 - Thr211) Accession # P04085 Human PDGF-B (Ser82 - Thr190) Accession # P01127 N-terminus C-terminus

source short :

E. coli

USD :

322 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage