Recombinant Human PDGF-AA Protein, CF | R&D Systems 221-AA product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human PDGF-AA Protein, CF

catalog :

221-AA

quantity :

10 ug (also 25 ug, 50 ug, 100 ug, 1000 ug)

price :

259 USD

citations: 38

Reference
Yoshida A, Takashima K, Shimonaga T, Kadokura M, Nagase S, Koda S. Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains. J Neurosci Methods. 2020;342:108798 pubmed publisher
Matjusaitis M, Wagstaff L, Martella A, Baranowski B, Blin C, Gogolok S, et al. Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors. Stem Cell Reports. 2019;13:1053-1067 pubmed publisher
Omar N, Lokanathan Y, Mohd Razi Z, Bt Haji Idrus R. The effects of Centella asiatica (L.) Urban on neural differentiation of human mesenchymal stem cells in vitro. BMC Complement Altern Med. 2019;19:167 pubmed publisher
Colunga T, Hayworth M, Kreß S, Reynolds D, Chen L, Nazor K, et al. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair. Cell Rep. 2019;26:2566-2579.e10 pubmed publisher
Rankin K, Mei F, Kim K, Shen Y, Mayoral S, Desponts C, et al. Selective Estrogen Receptor Modulators Enhance CNS Remyelination Independent of Estrogen Receptors. J Neurosci. 2019;39:2184-2194 pubmed publisher
Marton R, Miura Y, Sloan S, Li Q, Revah O, Levy R, et al. Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures. Nat Neurosci. 2019;22:484-491 pubmed publisher
Osipovitch M, Asenjo Martinez A, Mariani J, Cornwell A, Dhaliwal S, Zou L, et al. Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation. Cell Stem Cell. 2019;24:107-122.e7 pubmed publisher
Cheng C, Liao P, Ho A, Lim K, Chang J, Su Y, et al. STAT3 exacerbates survival of cancer stem-like tumorspheres in EGFR-positive colorectal cancers: RNAseq analysis and therapeutic screening. J Biomed Sci. 2018;25:60 pubmed publisher
Piñol Jurado P, Suarez Calvet X, Fernández Simón E, Gallardo E, de la Oliva N, Martínez Muriana A, et al. Nintedanib decreases muscle fibrosis and improves muscle function in a murine model of dystrophinopathy. Cell Death Dis. 2018;9:776 pubmed publisher
Göttle P, Manousi A, Kremer D, Reiche L, Hartung H, Küry P. Teriflunomide promotes oligodendroglial differentiation and myelination. J Neuroinflammation. 2018;15:76 pubmed publisher
Mamer S, Chen S, Weddell J, Palasz A, Wittenkeller A, Kumar M, et al. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics. Sci Rep. 2017;7:16439 pubmed publisher
Campbell A, Hogestyn J, Folts C, Lopez B, Proschel C, Mock D, et al. Expression of the Human Herpesvirus 6A Latency-Associated Transcript U94A Disrupts Human Oligodendrocyte Progenitor Migration. Sci Rep. 2017;7:3978 pubmed publisher
Santos R, Vadodaria K, Jaeger B, Mei A, Lefcochilos Fogelquist S, Mendes A, et al. Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells. Stem Cell Reports. 2017;8:1757-1769 pubmed publisher
Wu Y, Prager A, Boos S, Resch M, Brizic I, Mach M, et al. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry. PLoS Pathog. 2017;13:e1006281 pubmed publisher
Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, et al. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes. Am J Hum Genet. 2017;100:617-634 pubmed publisher
He H, Kuriyan A, Su C, Mahabole M, Zhang Y, Zhu Y, et al. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3. Sci Rep. 2017;7:43736 pubmed publisher
Papanikolaou M, Lewis A, Butt A. Store-operated calcium entry is essential for glial calcium signalling in CNS white matter. Brain Struct Funct. 2017;222:2993-3005 pubmed publisher
Yamashita T, Miyamoto Y, Bando Y, Ono T, Kobayashi S, Doi A, et al. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. PLoS ONE. 2017;12:e0171947 pubmed publisher
Tao C, Zhang X. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis. Cell Rep. 2016;17:1832-1844 pubmed publisher
Chen L, Ma S, Zhang P, Fan Z, Xiong M, Cheng G, et al. Neuroprotective effects of oligodendrocyte progenitor cell transplantation in premature rat brain following hypoxic-ischemic injury. PLoS ONE. 2015;10:e0115997 pubmed publisher
GÃ¶ttle P, Sabo J, Heinen A, Venables G, Torres K, Tzekova N, et al. Oligodendroglial maturation is dependent on intracellular protein shuttling. J Neurosci. 2015;35:906-19 pubmed publisher
Hayes B, Riehle K, Shimizu Albergine M, Bauer R, Hudkins K, Johansson F, et al. Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis. PLoS ONE. 2014;9:e92925 pubmed publisher
Roh J, Huang J, Hu W, Yang X, Jennings N, Sehgal V, et al. Biologic effects of platelet-derived growth factor receptor ? blockade in uterine cancer. Clin Cancer Res. 2014;20:2740-50 pubmed publisher
Roach E, Chakrabarti R, Park N, Keats E, Yip J, Chan N, et al. Intrinsic regulation of hemangioma involution by platelet-derived growth factor. Cell Death Dis. 2012;3:e328 pubmed publisher
Artus J, Panthier J, Hadjantonakis A. A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst. Development. 2010;137:3361-72 pubmed publisher
Jones J, Anderson J, Phuah C, Fox E, Selmaj K, Margolin D, et al. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity. Brain. 2010;133:2232-47 pubmed publisher
Ball S, Bayley C, Shuttleworth C, Kielty C. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells. Biochem J. 2010;427:29-40 pubmed publisher
Soroceanu L, Akhavan A, Cobbs C. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection. Nature. 2008;455:391-5 pubmed publisher
Wågsäter D, Zhu C, Björck H, Eriksson P. Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression. Atherosclerosis. 2009;202:415-23 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Rao R, Boyd J, Padmanabhan R, Chenoweth J, McKay R. Efficient serum-free derivation of oligodendrocyte precursors from neural stem cell-enriched cultures. Stem Cells. 2009;27:116-25 pubmed publisher
Dobson N, Zhou Y, Flint N, Armstrong R. Musashi1 RNA-binding protein regulates oligodendrocyte lineage cell differentiation and survival. Glia. 2008;56:318-30 pubmed
Li R, Maminishkis A, Wang F, Miller S. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Invest Ophthalmol Vis Sci. 2007;48:5722-32 pubmed
Ball S, Shuttleworth C, Kielty C. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J Cell Biol. 2007;177:489-500 pubmed
Jackson E, Garcia Verdugo J, Gil Perotin S, Roy M, Quinones Hinojosa A, Vandenberg S, et al. PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron. 2006;51:187-99 pubmed
Nagineni C, Kutty V, Detrick B, Hooks J. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J Cell Physiol. 2005;203:35-43 pubmed
Wen F, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, et al. Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads. Am J Respir Cell Mol Biol. 2004;30:816-22 pubmed
Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R. Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein. Int J Dev Biol. 2003;47:15-29 pubmed

product information

brand :

R&D Systems

master code :

221-AA

SKU :

221-AA-010

product name :

Recombinant Human PDGF-AA Protein, CF

description :

The Recombinant Human PDGF-AA Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human PDGF-AA Protein, CF has been validated for the following applications: Bioactivity.

target :

PDGF-AA

category :

Proteins and Enzymes

unit size :

10 ug (also 25 ug, 50 ug, 100 ug, 1000 ug)

buffer :

Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

theoretical molecular weight :

29 kDa

gene symbol :

PDGFA

details of functionality :

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al . (1985) Methods Enzymol. 109 :749. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002 ).

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

catalog number base :

221-AA

accessionNumbers :

P04085

applications :

Bioactivity

source :

E. coli -derived human PDGF-AA protein Ser87-Thr211, with and without an N-terminal Met

2020 USD :

259

2021 USD :

259 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.