Recombinant Human Legumain/Asparaginyl Endopeptidase, CF | R&D Systems 2199-CY product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Legumain/Asparaginyl Endopeptidase, CF

catalog :

2199-CY

quantity :

10 ug

price :

369 USD

citations: 7

Reference
Schlegel K, Awwad K, Heym R, Holzinger D, Doell A, Barghorn S, et al. N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain. Acta Neuropathol Commun. 2019;7:177 pubmed publisher
McGlinchey R, Lacy S, Huffer K, Tayebi N, Sidransky E, Lee J. C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem. 2019;294:9973-9984 pubmed publisher
Prabucka B, Mielecki M, Chojnacka M, Bielawski W, Czarnocki Cieciura M, Orzechowski S. Structural and functional characterization of the triticale (x Triticosecale Wittm.) phytocystatin TrcC-8 and its dimerization-dependent inhibitory activity. Phytochemistry. 2017;142:1-10 pubmed publisher
Perlenfein T, Mehlhoff J, Murphy R. Insights into the mechanism of cystatin C oligomer and amyloid formation and its interaction with β-amyloid. J Biol Chem. 2017;292:11485-11498 pubmed publisher
Mortensen S, Kidmose R, Petersen S, SzilÃ¡gyi Ã, ProhÃ¡szka Z, Andersen G. Structural Basis for the Function of Complement Component C4 within the Classical and Lectin Pathways of Complement. J Immunol. 2015;194:5488-96 pubmed publisher
Briggs J, Haugen M, Johansen H, Riker A, Abrahamson M, Fodstad Ø, et al. Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer. 2010;10:17 pubmed publisher
Clerin V, Shih H, Deng N, Hebert G, Resmini C, Shields K, et al. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis. 2008;201:53-66 pubmed publisher

product information

brand :

R&D Systems

master code :

2199-CY

SKU :

2199-CY-010

product name :

Recombinant Human Legumain/Asparaginyl Endopeptidase, CF

description :

The Recombinant Human Legumain/Asparaginyl Endopeptidase, CF from R&D Systems is derived from NS0. The Recombinant Human Legumain/Asparaginyl Endopeptidase, CF has been validated for the following applications: Enzyme Activity.

target :

Legumain/Asparaginyl Endopeptidase

category :

Proteins and Enzymes

unit size :

10 ug

buffer :

Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

60 kDa, reducing conditions

theoretical molecular weight :

49 kDa

gene symbol :

LGMN

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is 250 pmol/min/µg, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

2199-CY

accessionNumbers :

Q99538

applications :

Enzyme Activity

source :

Mouse myeloma cell line, NS0-derived human Legumain/Asparaginyl Endopeptidase protein Ile18-Tyr433, with an N-terminal 7-His tag

2020 USD :

369

2021 USD :

369 USD

alt names :

AEP, Asparaginyl endopeptidase, cysteine protease 1, legumain, LGMN, LGMN1, Protease, cysteine 1, protease, cysteine, 1 (legumain), PRSC1EC 3.4.22.34

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.