Recombinant Human Legumain/Asparaginyl Endopeptidase, CF | R&D Systems 2199-CY-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Legumain/Asparaginyl Endopeptidase, CF

catalog :

2199-CY-010

quantity :

10 ug

price :

468 USD

more info or order :

R&D Systems product webpage

citations: 8

Reference
Bu x161 a M, Matou x161 kov xe1 Z, Barto x161 ov xe1 Sojkov xe1 P, Pachl P, x158 ez xe1 x10d ov xe1 P, Eichenberger R, et al. An evolutionary molecular adaptation of an unusual stefin from the liver fluke Fasciola hepatica redefines the cystatin superfamily. J Biol Chem. 2023;299:102970 pubmed publisher
Sun H, Ma W, Duan S, Huang J, Jia R, Cheng H, et al. An endogenous stimulus detonated nanocluster-bomb for contrast-enhanced cancer imaging and combination therapy. Chem Sci. 2021;12:12118-12129 pubmed publisher
Schlegel K, Awwad K, Heym R, Holzinger D, Doell A, Barghorn S, et al. N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain. Acta Neuropathol Commun. 2019;7:177 pubmed publisher
Prabucka B, Mielecki M, Chojnacka M, Bielawski W, Czarnocki Cieciura M, Orzechowski S. Structural and functional characterization of the triticale (x Triticosecale Wittm.) phytocystatin TrcC-8 and its dimerization-dependent inhibitory activity. Phytochemistry. 2017;142:1-10 pubmed publisher
Perlenfein T, Mehlhoff J, Murphy R. Insights into the mechanism of cystatin C oligomer and amyloid formation and its interaction with β-amyloid. J Biol Chem. 2017;292:11485-11498 pubmed publisher
Mortensen S, Kidmose R, Petersen S, SzilÃ¡gyi Ã, ProhÃ¡szka Z, Andersen G. Structural Basis for the Function of Complement Component C4 within the Classical and Lectin Pathways of Complement. J Immunol. 2015;194:5488-96 pubmed publisher
Briggs J, Haugen M, Johansen H, Riker A, Abrahamson M, Fodstad Ø, et al. Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer. 2010;10:17 pubmed publisher
Clerin V, Shih H, Deng N, Hebert G, Resmini C, Shields K, et al. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis. 2008;201:53-66 pubmed publisher

product information

brand :

R&D Systems

master code :

2199-CY

SKU :

2199-CY-010

product name :

Recombinant Human Legumain/Asparaginyl Endopeptidase, CF

unit size :

10 ug

seo description :

The Recombinant Human Legumain/Asparaginyl Endopeptidase, CF from R&D Systems is derived from NS0. The Recombinant Human Legumain/Asparaginyl Endopeptidase, CF has been validated for the following applications: Enzyme Activity.

target :

Legumain/Asparaginyl Endopeptidase

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl and Glycerol.

conjugate :

Unconjugated

dilution :

Enzyme Activity

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

60 kDa, reducing conditions

theoretical molecular weight :

49 kDa

gene symbol :

LGMN

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is 250 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

AAH03061.1

applications :

Enzyme Activity

source :

NS0-derived Recombinant Human Legumain/Asparaginyl Endopeptidase, CF

USD :

468 USD

alt names :

AEP, Asparaginyl endopeptidase, cysteine protease 1, legumain, LGMN, LGMN1, Protease, cysteine 1, protease, cysteine, 1 (legumain), PRSC1EC 3.4.22.34

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage