domestic goat polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, proximity ligation assay
citations: 19
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, proximity ligation assay
citations: 19
Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, Catalog # 416-ML ), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR ) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/ RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 ╡g/mL.
quantity: 100 ug (also 25 ug)
price: 519 USD
to the supplier
domestic goat polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunocytochemistry
citations: 2
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunocytochemistry
citations: 2
rat monoclonal (88227)
reactivity: mouse
application: western blot, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 2
reactivity: mouse
application: western blot, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 2
mouse monoclonal (685809)
reactivity: human, mouse
application: western blot
citations: 1
reactivity: human, mouse
application: western blot
citations: 1
Detection of Human TRANCE/TNFSF11/RANK L by Western Blot. Western blot shows lysates of human primary differentiated CD4+ Th2 cells. PVDF Membrane was probed with 2 ╡g/mL of Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6263) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for TRANCE/TNFSF11/RANK L at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 439 USD
to the supplier