product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-12 Protein
catalog :
219-IL-005
quantity :
5 ug (also 25 ug)
price :
374 USD
more info or order :
citations: 143
Reference
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Wang B, Niu D, Lam T, Xiao Z, Ren E. Mapping the p53 transcriptome universe using p53 natural polymorphs. Cell Death Differ. 2014;21:521-32 pubmed publisher
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You C, Shi M, Liu Y, Cao M, Luo R, Hermonat P. AAV2/IL-12 gene delivery into dendritic cells (DC) enhances CTL stimulation above other IL-12 applications: Evidence for IL-12 intracrine activity in DC. Oncoimmunology. 2012;1:847-855 pubmed
Flanagan K, FITZGERALD K, Baker J, Regnstrom K, Gardai S, Bard F, et al. Laminin-411 is a vascular ligand for MCAM and facilitates TH17 cell entry into the CNS. PLoS ONE. 2012;7:e40443 pubmed publisher
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Le Garff Tavernier M, Decocq J, de Romeuf C, Parizot C, Dutertre C, Chapiro E, et al. Analysis of CD16+CD56dim NK cells from CLL patients: evidence supporting a therapeutic strategy with optimized anti-CD20 monoclonal antibodies. Leukemia. 2011;25:101-9 pubmed publisher
Ramirez J, Brembilla N, Sorg O, Chicheportiche R, Matthes T, Dayer J, et al. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells. Eur J Immunol. 2010;40:2450-9 pubmed publisher
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Ramana Rao P, Rajasekaran S, Raja A. Natural killer cell-mediated cytokine response among HIV-positive south Indians with pulmonary tuberculosis. J Interferon Cytokine Res. 2010;30:33-42 pubmed publisher
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Brucklacher Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression. J Immunol. 2009;183:5494-501 pubmed publisher
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product information
brand :
R&D Systems
master code :
219-IL
SKU :
219-IL-005
product name :
Recombinant Human IL-12 Protein
unit size :
5 ug (also 25 ug)
seo description :
The Recombinant Human IL-12 Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human IL-12 Protein has been validated for the following applications: Bioactivity.
target :
IL-12
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
41 kDa (p40) 29 kDa (p35), reducing conditions
theoretical molecular weight :
34.7 kDa (p40) 22.5 kDa (p35)
gene symbol :
IL12A
details of functionality :
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. etáal . (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. etáal . (eds): IRL Press. 272. The ED50 for this effect is 0.01-0.05 ng/mL.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P29460 (p40) P29459 (p35)
applications :
Bioactivity
source :
Sf 21 (baculovirus)-derived Recombinant Human IL-12 Protein
extended description :
Analyzed by SEC-MALS
USD :
374 USD
product details :
Analyzed by SEC-MALS
alt names :
CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.