product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-10 Protein
catalog :
217-IL-005
quantity :
5 ug (also 10 ug, 25 ug, 50 ug, 250 ug, 1000 ug)
price :
323 USD
more info or order :
citations: 102
Reference
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Seyerl M, Kirchberger S, Majdic O, Seipelt J, Jindra C, Schrauf C, et al. Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Eur J Immunol. 2010;40:321-9 pubmed publisher
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Park Min K, Ji J, Antoniv T, Reid A, Silver R, Humphrey M, et al. IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression. J Immunol. 2009;183:2444-55 pubmed publisher
Chang W, Barry P, Szubin R, Wang D, Baumgarth N. Human cytomegalovirus suppresses type I interferon secretion by plasmacytoid dendritic cells through its interleukin 10 homolog. Virology. 2009;390:330-7 pubmed publisher
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Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, et al. Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin Exp Immunol. 2009;155:266-74 pubmed publisher
Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, et al. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. J Immunol. 2008;181:2772-80 pubmed
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van der Vliet H, Wang R, Yue S, Koon H, Balk S, Exley M. Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells. J Immunol. 2008;180:7287-93 pubmed
Kato A, Peters A, Suh L, Carter R, Harris K, Chandra R, et al. Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. J Allergy Clin Immunol. 2008;121:1385-92, 1392.e1-2 pubmed publisher
Liu T, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, et al. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur J Immunol. 2008;38:1012-23 pubmed publisher
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Gleissner C, Zastrow A, Klingenberg R, Kluger M, Konstandin M, Celik S, et al. IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells. Eur J Immunol. 2007;37:177-92 pubmed
Wan B, Nie H, Liu A, Feng G, He D, Xu R, et al. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis. J Immunol. 2006;177:8844-50 pubmed
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Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, et al. Selective regulation of IL-10 signaling and function by zymosan. J Immunol. 2006;176:4785-92 pubmed
Hsieh F, Sharma P, Gibbons A, Goggans T, Erzurum S, Haque S. Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells. Proc Natl Acad Sci U S A. 2005;102:14380-5 pubmed
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image
image 1 :
R&D Systems 217-IL-005 image 1
Recombinant Human IL-10 Protein Bioactivity
product information
brand :
R&D Systems
master code :
217-IL
SKU :
217-IL-005
product name :
Recombinant Human IL-10 Protein
description :
The Recombinant Human IL-10 Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human IL-10 Protein has been validated for the following applications: Bioactivity.
target :
IL-10
category :
Proteins and Enzymes
sizes available :
5 ug (also 10 ug, 25 ug, 50 ug, 250 ug, 1000 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
theoretical molecular weight :
18.6 kDa
gene symbol :
IL10
details of functionality :
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L.  et al . (1991) J. Exp. Med. 173 :507. The ED50 for this effect is 0.075-0.750 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P22301
applications :
Bioactivity
extended description :
E. Coli Derived Version Also Available
source :
Sf 21 (baculovirus)-derived Recombinant Human IL-10 Protein
USD :
323
USD 2023 :
323 USD
product details :
E. Coli Derived Version Also Available
alt names :
CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.