domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 24
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 24
Detection of Tie-2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were unstimulated (light orange filled histogram) or treated with 100 ╡M pervanadate for 15 minutes (dark orange filled histogram), then stained with Human/Mouse Phospho- Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110 ). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
quantity: 50 ug (also 25 ug)
price: 399 USD
to the supplier
domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 22
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 22
Detection of Human Tie-2 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 ╡g/mL of Goat Anti-Human Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for Tie-2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 509 USD
to the supplier
domestic goat polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry, neutralization, immunohistochemistry - frozen section, immunohistochemistry - free floating section, immunohistochemistry knockout validation
citations: 28
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry, neutralization, immunohistochemistry - frozen section, immunohistochemistry - free floating section, immunohistochemistry knockout validation
citations: 28
domestic rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunohistochemistry
citations: 4
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunohistochemistry
citations: 4
Detection of Mouse Phospho-Tie-2 (Y1102/Y1100) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line transfected with mouse Tie-2 untreated (-) or treated (+) with 600 ng/mL Recombinant Human Angiopoietin-1 (Catalog # 923-AN ) for 5 minutes. PVDF membrane was probed with 1 ╡g/mL of Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y1102/Y1100) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3909) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008 ). A specific band was detected for Phospho-Tie-2 (Y1102/1100) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 409 USD
to the supplier
domestic goat polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
citations: 1
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
citations: 1