product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Complement Component C5a Protein
catalog :
2150-C5-025
quantity :
25 ug
price :
408 USD
more info or order :
citations: 40
Reference
Senent Y, Rem xed rez A, Rep xe1 raz D, Llopiz D, Celias D, Sainz C, et al. The C5a/C5aR1 Axis Promotes Migration of Tolerogenic Dendritic Cells to Lymph Nodes, Impairing the Anticancer Immune Response. Cancer Immunol Res. 2025;13:384-399 pubmed publisher
Lilley R, Taylor K, Wildman S, Peppiatt Wildman C. Inflammatory mediators act at renal pericytes to elicit contraction of vasa recta and reduce pericyte density along the kidney medullary vascular network. Front Physiol. 2023;14:1194803 pubmed publisher
Xiong W, Jia L, Liang J, Cai Y, Chen Y, Nie Y, et al. Investigation into the anti-airway inflammatory role of the PI3Kγ inhibitor JN-PK1: An in vitro and in vivo study. Int Immunopharmacol. 2022;111:109102 pubmed publisher
Llori xe1 n Salvador M, Byrne E, Szczepan M, Little K, Chen M, Xu H. Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells. J Neuroinflammation. 2022;19:182 pubmed publisher
Brandolini L, d Angelo M, Novelli R, Castelli V, Giorgio C, Sirico A, et al. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions. Cell Death Dis. 2022;13:500 pubmed publisher
Consonni F, Bleve A, Totaro M, Storto M, Kunderfranco P, Termanini A, et al. Heme catabolism by tumor-associated macrophages controls metastasis formation. Nat Immunol. 2021;22:595-606 pubmed publisher
van den Bos E, Ambrosy B, Horsthemke M, Walbaum S, Bachg A, Wettschureck N, et al. Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis. J Biol Chem. 2020;295:7726-7742 pubmed publisher
Schmider A, Bauer N, Sunwoo H, Godin M, Ellis G, Lee J, et al. Two- and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils. J Biol Chem. 2020;295:5761-5770 pubmed publisher
Hsu B, Roy J, Mouhanna J, Rayes R, Ramsay L, Tabariès S, et al. C3a elicits unique migratory responses in immature low-density neutrophils. Oncogene. 2020;39:2612-2623 pubmed publisher
Muth C, Hartmann A, Sepulveda Falla D, Glatzel M, Krasemann S. Phagocytosis of Apoptotic Cells Is Specifically Upregulated in ApoE4 Expressing Microglia in vitro. Front Cell Neurosci. 2019;13:181 pubmed publisher
Kobayakawa K, Ohkawa Y, Yoshizaki S, Tamaru T, Saito T, Kijima K, et al. Macrophage centripetal migration drives spontaneous healing process after spinal cord injury. Sci Adv. 2019;5:eaav5086 pubmed publisher
Funk N, Munz M, Ott T, Brockmann K, Wenninger Weinzierl A, Kühn R, et al. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4. Sci Rep. 2019;9:4515 pubmed publisher
Arandjelovic S, Perry J, Lucas C, Penberthy K, Kim T, Zhou M, et al. A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis. Nat Immunol. 2019;20:141-151 pubmed publisher
Crowley M, Delgado O, Will Orrego A, Buchanan N, Anderson K, Jaffee B, et al. Induction of Ocular Complement Activation by Inflammatory Stimuli and Intraocular Inhibition of Complement Factor D in Animal Models. Invest Ophthalmol Vis Sci. 2018;59:940-951 pubmed publisher
Toomey C, Landowski M, Klingeborn M, Kelly U, Deans J, Dong H, et al. Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci. 2018;59:662-673 pubmed publisher
Herrmann J, Muenstermann M, Strobel L, Schubert Unkmeir A, Woodruff T, Gray Owen S, et al. Complement C5a Receptor 1 Exacerbates the Pathophysiology of N. meningitidis Sepsis and Is a Potential Target for Disease Treatment. MBio. 2018;9: pubmed publisher
Syed S, Rau E, Ziegelmann M, Sogkas G, Brüne B, Schmidt R. C5aR activation in the absence of C5a: A new disease mechanism of autoimmune hemolytic anemia in mice. Eur J Immunol. 2018;48:696-704 pubmed publisher
Wang Y, Lai P, Chen X, He C, Huang X, Geng S, et al. Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg. Sci Rep. 2017;7:3603 pubmed publisher
Bergdolt S, Kovtun A, Hägele Y, Liedert A, Schinke T, Amling M, et al. Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing. PLoS ONE. 2017;12:e0179512 pubmed publisher
Markiewski M, Vadrevu S, Sharma S, Chintala N, Ghouse S, Cho J, et al. The Ribosomal Protein S19 Suppresses Antitumor Immune Responses via the Complement C5a Receptor 1. J Immunol. 2017;198:2989-2999 pubmed publisher
Shutov L, Warwick C, Shi X, Gnanasekaran A, Shepherd A, Mohapatra D, et al. The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1. J Neurosci. 2016;36:5055-70 pubmed publisher
Contractor T, Kobayashi S, da Silva E, Clausen R, Chan C, Vosburgh E, et al. Sexual dimorphism of liver metastasis by murine pancreatic neuroendocrine tumors is affected by expression of complement C5. Oncotarget. 2016;7:30585-96 pubmed publisher
Hillgruber C, Pöppelmann B, Weishaupt C, Steingräber A, Wessel F, Berdel W, et al. Blocking neutrophil diapedesis prevents hemorrhage during thrombocytopenia. J Exp Med. 2015;212:1255-66 pubmed publisher
Yatime L, Maasch C, Hoehlig K, Klussmann S, Andersen G, Vater A. Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun. 2015;6:6481 pubmed publisher
Piao C, Cai L, Qiu S, Jia L, Song W, Du J. Complement 5a Enhances Hepatic Metastases of Colon Cancer via Monocyte Chemoattractant Protein-1-mediated Inflammatory Cell Infiltration. J Biol Chem. 2015;290:10667-76 pubmed publisher
Price P, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, et al. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol. 2015;194:1164-8 pubmed publisher
Pazos M, Pirzai W, Yonker L, Morisseau C, Gronert K, Hurley B. Distinct cellular sources of hepoxilin A3 and leukotriene B4 are used to coordinate bacterial-induced neutrophil transepithelial migration. J Immunol. 2015;194:1304-15 pubmed publisher
Grailer J, Fattahi F, Dick R, Zetoune F, Ward P. Cutting edge: critical role for C5aRs in the development of septic lymphopenia in mice. J Immunol. 2015;194:868-72 pubmed publisher
Vijayan S, Asare Y, Grommes J, Soehnlein O, Lutgens E, Shagdarsuren G, et al. High expression of C5L2 correlates with high proinflammatory cytokine expression in advanced human atherosclerotic plaques. Am J Pathol. 2014;184:2123-33 pubmed publisher
Kim J, Oh S, Ahn S, Chung D. IFN-?-producing NKT cells exacerbate sepsis by enhancing C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils. Eur J Immunol. 2014;44:2025-35 pubmed publisher
Hsu W, Yang F, Lin C, Hsieh S, Chen N. C5L2 is required for C5a-triggered receptor internalization and ERK signaling. Cell Signal. 2014;26:1409-19 pubmed publisher
Bosmann M, Grailer J, Ruemmler R, Russkamp N, Zetoune F, Sarma J, et al. Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury. FASEB J. 2013;27:5010-21 pubmed publisher
Hoehlig K, Maasch C, Shushakova N, Buchner K, Huber Lang M, Purschke W, et al. A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther. 2013;21:2236-46 pubmed publisher
Bosmann M, Haggadone M, Zetoune F, Sarma J, Ward P. The interaction between C5a and both C5aR and C5L2 receptors is required for production of G-CSF during acute inflammation. Eur J Immunol. 2013;43:1907-13 pubmed publisher
Abe T, Hosur K, Hajishengallis E, Reis E, Ricklin D, Lambris J, et al. Local complement-targeted intervention in periodontitis: proof-of-concept using a C5a receptor (CD88) antagonist. J Immunol. 2012;189:5442-8 pubmed publisher
Gunn L, Ding C, Liu M, Ma Y, Qi C, Cai Y, et al. Opposing roles for complement component C5a in tumor progression and the tumor microenvironment. J Immunol. 2012;189:2985-94 pubmed publisher
Heimbach L, Li Z, Berkowitz P, Zhao M, Li N, Rubenstein D, et al. The C5a receptor on mast cells is critical for the autoimmune skin-blistering disease bullous pemphigoid. J Biol Chem. 2011;286:15003-9 pubmed publisher
Liang S, Krauss J, Domon H, McIntosh M, Hosur K, Qu H, et al. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss. J Immunol. 2011;186:869-77 pubmed publisher
Chen N, Mirtsos C, Suh D, Lu Y, Lin W, McKerlie C, et al. C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a. Nature. 2007;446:203-7 pubmed
Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, et al. Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice. Immunol Lett. 2007;109:56-63 pubmed
product information
master code :
2150-C5
SKU :
2150-C5-025
product name :
Recombinant Mouse Complement Component C5a Protein
unit size :
25 ug
description :
The Recombinant Mouse Complement Component C5a Protein from R&D Systems is derived from E. coli. The Recombinant Mouse Complement Component C5a Protein has been validated for the following applications: Bioactivity.
target :
Complement Component C5a
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Mouse
theoretical molecular weight :
9.0 kDa
gene symbol :
Hc
details of functionality :
Measured by its ability to induce N-acetyl┐▀-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29: 9274. The ED50 for this effect is 5-20 ng/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Mouse Complement Component C5a Protein Bioactivity
accessionNumbers :
P06684
applications :
Bioactivity
source long :
E. coli -derived mouse Complement Component C5a protein Asn679-Arg755, with an N-terminal Met
source short :
E. coli
USD :
408 USD
alt names :
C5, C5A, HE
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.