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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human G-CSF Protein
catalog :
214-CS-005
quantity :
5 ug (also 25 ug)
price :
252 USD
more info or order :
citations: 56
Reference
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Hong J, Kim J, Kang K, Lee B, Park Y, Kim B. Generation of a human induced pluripotent stem cell line KUMi006 from a patient with multiple myeloma. Stem Cell Res. 2022;61:102767 pubmed publisher
Kim J, Kang K, Lee B, Park Y, Jung Sung H, Kim B. A human pluripotent stem cell line KUMi004-A generated from a patient with chronic lymphocytic leukemia. Stem Cell Res. 2022;60:102668 pubmed publisher
Kim J, Kang K, Lee B, Park Y, Kim B. Generation of induced pluripotent stem cell line KUMi002-A with normal karyotype from a patient with Philadelphia chromosome-positive chronic myeloid leukemia. Stem Cell Res. 2021;55:102465 pubmed publisher
Li J, Wuethrich A, Sina A, Cheng H, Wang Y, Behren A, et al. A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy. Nat Commun. 2021;12:1087 pubmed publisher
Lee S, Kim J, Kang K, Lee B, Kim B. Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation. Stem Cell Res. 2020;49:102030 pubmed publisher
Lee S, Kim J, Kang K, Park Y, Kim B. Supporting data on enhanced reprogramming of human CD34+ hematopoietic stem cells to induced pluripotent stem cells using human placenta-derived cell conditioned medium. Data Brief. 2020;32:106140 pubmed publisher
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Lee S, Kang K, Kim J, Park Y, Kim B. Generation of an induced pluripotent stem cell line KUMCi001-A from CD34+ bone marrow cells of a patient with acute lymphoblastic leukemia using human placenta-derived cell conditioned medium. Stem Cell Res. 2020;47:101913 pubmed publisher
Kang K, Lee S, Kim J, Lee B, Kim S, Park Y, et al. Etoposide-mediated interleukin-8 secretion from bone marrow stromal cells induces hematopoietic stem cell mobilization. BMC Cancer. 2020;20:619 pubmed publisher
Yu H, Hu W, Song X, Descalzi Montoya D, Yang Z, Korngold R, et al. Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria. Int J Mol Sci. 2020;21: pubmed publisher
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Wakamatsu M, Okuno Y, Murakami N, Miwata S, Kitazawa H, Narita K, et al. Detection of subclonal SETBP1 and JAK3 mutations in juvenile myelomonocytic leukemia using droplet digital PCR. Leukemia. 2021;35:259-263 pubmed publisher
Nasri M, Ritter M, Mir P, Dannenmann B, Aghaallaei N, Amend D, et al. CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients. Haematologica. 2019;: pubmed publisher
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Lee M, McCubbin J, Christensen A, Poole D, Rajasekhar P, Lieu T, et al. G-CSF Receptor Blockade Ameliorates Arthritic Pain and Disease. J Immunol. 2017;198:3565-3575 pubmed publisher
Schrimpf C, Wrede C, Glage S, Hegermann J, Backhaus S, Blasczyk R, et al. Differentiation of induced pluripotent stem cell-derived neutrophil granulocytes from common marmoset monkey (Callithrix jacchus). Transfusion. 2017;57:60-69 pubmed publisher
Kim H, Lee J, Heo H, Yang S, Ha K, Park W, et al. Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway. Cell Biosci. 2016;6:50 pubmed publisher
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Lin H, Masaki H, Yamaguchi T, Wada T, Yachie A, Nishimura K, et al. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol Ther Methods Clin Dev. 2015;2:15046 pubmed publisher
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Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, et al. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML). PLoS ONE. 2015;10:e0122983 pubmed publisher
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Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N. Granulocyte-Colony Stimulating Factor related pathways tested on an endometrial ex-vivo model. PLoS ONE. 2014;9:e102286 pubmed publisher
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Xin H, Lu R, Lee H, Zhang W, Zhang C, Deng J, et al. G-protein-coupled receptor agonist BV8/prokineticin-2 and STAT3 protein form a feed-forward loop in both normal and malignant myeloid cells. J Biol Chem. 2013;288:13842-9 pubmed publisher
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Niwa A, Heike T, Umeda K, Oshima K, Kato I, Sakai H, et al. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE. 2011;6:e22261 pubmed publisher
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Shao H, Xu X, Jing N, Tweardy D. Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744. J Immunol. 2006;176:2933-41 pubmed
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Cox G, Smith D, Carlson S, Bendele A, Chlipala E, Doherty D. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein. Exp Hematol. 2004;32:441-9 pubmed
Uchida K, Nakata K, Trapnell B, Terakawa T, Hamano E, Mikami A, et al. High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis. Blood. 2004;103:1089-98 pubmed
product information
master code :
214-CS
SKU :
214-CS-005
product name :
Recombinant Human G-CSF Protein
unit size :
5 ug (also 25 ug)
description :
The Recombinant Human G-CSF Protein from R&D Systems is derived from E. coli. The Recombinant Human G-CSF Protein has been validated for the following applications: Bioactivity.
target :
G-CSF
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in Acetic Acid with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
17-19 kDa, reducing conditions
theoretical molecular weight :
18.8 kDa
gene symbol :
CSF3
details of functionality :
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. Shirafuji,áN. etáal . (1989) Exp. Hematol. 17 :116. The ED50 for this effect is 10-60 pg/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human G-CSF Protein Bioactivity
accessionNumbers :
NP_757373
applications :
Bioactivity
source long :
E. coli -derived human G-CSF protein Thr31-Pro204, with an N-terminal Met
source short :
E. coli
USD :
252 USD
alt names :
C17orf33, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), CSF3, CSF3OS, Filgrastim, GCSF, G-CSF, GCSFlenograstim, granulocyte colony-stimulating factor, Lenograstim, MGC45931, pluripoietin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.