Recombinant Human Glypican 3 Protein | R&D Systems 2119-GP-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Glypican 3 Protein

catalog :

2119-GP-050

quantity :

50 ug

price :

458 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed publisher
Miura M, Fujinami N, Shimizu Y, Mizuno S, Saito K, Suzuki T, et al. Usefulness of plasma full-length glypican-3 as a predictive marker of hepatocellular carcinoma recurrence after radial surgery. Oncol Lett. 2020;19:2657-2666 pubmed publisher
Ueda T, Kumagai A, Iriguchi S, Yasui Y, Miyasaka T, Nakagoshi K, et al. Non-clinical efficacy, safety and stable clinical cell processing of induced pluripotent stem cell-derived anti-glypican-3 chimeric antigen receptor-expressing natural killer/innate lymphoid cells. Cancer Sci. 2020;111:1478-1490 pubmed publisher
Gao W, Xu Y, Liu J, Ho M. Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate. Sci Rep. 2016;6:26245 pubmed publisher
Sawada Y, Yoshikawa T, Nobuoka D, Shirakawa H, Kuronuma T, Motomura Y, et al. Phase I trial of a glypican-3-derived peptide vaccine for advanced hepatocellular carcinoma: immunologic evidence and potential for improving overall survival. Clin Cancer Res. 2012;18:3686-96 pubmed publisher
Komori H, Nakatsura T, Senju S, Yoshitake Y, Motomura Y, Ikuta Y, et al. Identification of HLA-A2- or HLA-A24-restricted CTL epitopes possibly useful for glypican-3-specific immunotherapy of hepatocellular carcinoma. Clin Cancer Res. 2006;12:2689-97 pubmed
Motomura Y, Senju S, Nakatsura T, Matsuyoshi H, Hirata S, Monji M, et al. Embryonic stem cell-derived dendritic cells expressing glypican-3, a recently identified oncofetal antigen, induce protective immunity against highly metastatic mouse melanoma, B16-F10. Cancer Res. 2006;66:2414-22 pubmed

image

image 1 :

Graph showing bioactivity of human Glypican-3 protein

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

2119-GP

SKU :

2119-GP-050

product name :

Recombinant Human Glypican 3 Protein

Description :

The Recombinant Human Glypican 3 Protein from R&D Systems is derived from NS0. The Recombinant Human Glypican 3 Protein has been validated for the following applications: Binding Activity.

target :

Glypican 3

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

60-100 kDa, non-reducing conditions

theoretical molecular weight :

61.6 kDa

gene symbol :

GPC3

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Human Glypican 3 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB ) that produces 50% of the optimal binding response is approximately 0.6-3 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P51654.1

applications :

Binding Activity

source :

NS0-derived Recombinant Human Glypican 3 Protein

USD :

458 USD

alt names :

DGSX, glypican 3, glypican proteoglycan 3, glypican-3, GPC3, GTR2-2, heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, OCI5, OCI-5, secreted glypican-3, SGB, SGBS, SGBS1SDYS

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage