Recombinant Human TNF-alpha GMP Protein, CF | R&D Systems 210-GMP-100 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human TNF-alpha GMP Protein, CF

catalog :

210-GMP-100

quantity :

100 ug (also 2 mg)

price :

1023 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Kang I, Jang J, Kim C. Opposing roles of hematopoietic-specific small GTPase Rac2 and the guanine nucleotide exchange factor Vav1 in osteoclast differentiation. Sci Rep. 2020;10:7024 pubmed publisher
Lei W, Duan R, Li J, Liu X, Huston A, Boyce B, et al. The IAP Antagonist SM-164 Eliminates Triple-Negative Breast Cancer Metastasis to Bone and Lung in Mice. Sci Rep. 2020;10:7004 pubmed publisher

image

image 1 :

Recombinant Human TNF-alpha GMP Protein Bioactivity

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

210-GMP

SKU :

210-GMP-100

product name :

Recombinant Human TNF-alpha GMP Protein, CF

Description :

The Recombinant Human TNF-alpha GMP Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human TNF-alpha GMP Protein, CF has been validated for the following applications: Bioactivity.

target :

TNF-alpha

category :

Proteins and Enzymes

unit size :

100 ug (also 2 mg)

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE with silver staining.

species :

Human

observed molecular weight :

17 kDa, reducing conditions

theoretical molecular weight :

17 kDa

gene symbol :

TNF

details of functionality :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-alpha GMP is 4.3 x 107 IU/mg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154).

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P01375.1

applications :

Bioactivity

source :

E. coli-derived Recombinant Human TNF-alpha GMP Protein

extended description :

Animal-Free.

USD :

1023 USD

product details :

Animal-Free.

alt names :

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage