product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-8/CXCL8 Protein, CF
catalog :
208-IL-010/CF
quantity :
10 ug (also 50 ug)
price :
172 USD
more info or order :
citations: 120
Reference
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Coperchini F, Croce L, Denegri M, Awwad O, Ngnitejeu S, Muzza M, et al. The BRAF-inhibitor PLX4720 inhibits CXCL8 secretion in BRAFV600E mutated and normal thyroid cells: a further anti-cancer effect of BRAF-inhibitors. Sci Rep. 2019;9:4390 pubmed publisher
Tabei Y, Fukui H, Nishioka A, Hagiwara Y, Sato K, Yoneda T, et al. Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells. Sci Rep. 2019;9:2224 pubmed publisher
Ning Y, Feng W, Cao X, Ren K, Quan M, Chen A, et al. Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis. J Exp Clin Cancer Res. 2019;38:19 pubmed publisher
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Fraser J, Kemp S, Young L, Ross M, Prach M, Hutchison G, et al. Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations. Sci Rep. 2018;8:7506 pubmed publisher
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Krawczyk K, Nilsson H, Allaoui R, Lindgren D, Arvidsson M, Leandersson K, et al. Papillary renal cell carcinoma-derived chemerin, IL-8, and CXCL16 promote monocyte recruitment and differentiation into foam-cell macrophages. Lab Invest. 2017;97:1296-1305 pubmed publisher
Shen L, Zhang P, Zhang S, Xie L, Yao L, Lang W, et al. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Exp Ther Med. 2017;13:3021-3031 pubmed publisher
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Millrud C, Kågedal A, Kumlien Georén S, Winqvist O, Uddman R, Razavi R, et al. NET-producing CD16high CD62Ldim neutrophils migrate to tumor sites and predict improved survival in patients with HNSCC. Int J Cancer. 2017;140:2557-2567 pubmed publisher
Mikuła Pietrasik J, Uruski P, Sosińska P, Maksin K, Piotrowska Kempisty H, Kucińska M, et al. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases. Cell Death Dis. 2016;7:e2565 pubmed publisher
Allen S, Chen Y, Madden J, Fournier C, Altemus M, Hiziroglu A, et al. Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling. Sci Rep. 2016;6:39190 pubmed publisher
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Bastidas Coral A, Bakker A, Zandieh Doulabi B, Kleverlaan C, Bravenboer N, Forouzanfar T, et al. Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells. Stem Cells Int. 2016;2016:1318256 pubmed publisher
Grimberg Peters D, Büren C, Windolf J, Wahlers T, Paunel Görgülü A. Hyperbaric Oxygen Reduces Production of Reactive Oxygen Species in Neutrophils from Polytraumatized Patients Yielding in the Inhibition of p38 MAP Kinase and Downstream Pathways. PLoS ONE. 2016;11:e0161343 pubmed publisher
Robert S, Gicquel T, Bodin A, Lagente V, Boichot E. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1β or TNF-α Release from Human Hepatic Stellate Cells. PLoS ONE. 2016;11:e0153118 pubmed publisher
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Picon Ruiz M, Pan C, Drews Elger K, Jang K, Besser A, Zhao D, et al. Interactions between Adipocytes and Breast Cancer Cells Stimulate Cytokine Production and Drive Src/Sox2/miR-302b-Mediated Malignant Progression. Cancer Res. 2016;76:491-504 pubmed publisher
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Hazeldine J, Harris P, Chapple I, Grant M, Greenwood H, Livesey A, et al. Impaired neutrophil extracellular trap formation: a novel defect in the innate immune system of aged individuals. Aging Cell. 2014;13:690-8 pubmed publisher
Mamik M, Ghorpade A. Chemokine CXCL8 promotes HIV-1 replication in human monocyte-derived macrophages and primary microglia via nuclear factor-?B pathway. PLoS ONE. 2014;9:e92145 pubmed publisher
Gharaee Kermani M, Mehra R, Robinson D, Wei J, Macoska J. Complex cellular composition of solitary fibrous tumor of the prostate. Am J Pathol. 2014;184:732-9 pubmed publisher
Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V. Neutrophil microparticles modulate cytokine production by natural killer cells. Cytokine. 2014;65:126-9 pubmed publisher
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Mondrinos M, Zhang T, Sun S, Kennedy P, King D, Wolfson M, et al. Pulmonary endothelial protein kinase C-delta (PKC?) regulates neutrophil migration in acute lung inflammation. Am J Pathol. 2014;184:200-13 pubmed publisher
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Kothari H, Rao L, Vankayalapati R, Pendurthi U. Mycobacterium tuberculosis infection and tissue factor expression in macrophages. PLoS ONE. 2012;7:e45700 pubmed publisher
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Sahlander K, Larsson K, Palmberg L. Daily exposure to dust alters innate immunity. PLoS ONE. 2012;7:e31646 pubmed publisher
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Kinzfogl J, Hangoc G, Broxmeyer H. Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells. Blood. 2011;118:565-75 pubmed publisher
Burton V, Butler L, McGettrick H, Stone P, Jeffery H, Savage C, et al. Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture. Exp Cell Res. 2011;317:276-92 pubmed publisher
Russo R, Garcia C, Barcelos L, Rachid M, Guabiraba R, Roffe E, et al. Phosphoinositide 3-kinase ? plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice. J Leukoc Biol. 2011;89:269-82 pubmed publisher
Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic I, Socher I, et al. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood. 2010;116:841-9 pubmed publisher
Morizane S, Yamasaki K, Kabigting F, Gallo R. Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid. J Invest Dermatol. 2010;130:1297-306 pubmed publisher
Reeves E, Williamson M, Byrne B, Bergin D, Smith S, Greally P, et al. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis. J Immunol. 2010;184:1642-52 pubmed publisher
Leick M, Catusse J, Follo M, Nibbs R, Hartmann T, Veelken H, et al. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology. 2010;129:536-46 pubmed publisher
van der Veen B, Petersen A, Belperio J, Satchell S, Mathieson P, Molema G, et al. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis. Clin Exp Immunol. 2009;158:143-53 pubmed publisher
Li Z, Alam S, Wang J, Sandstrom C, Janciauskiene S, Mahadeva R. Oxidized {alpha}1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema. Am J Physiol Lung Cell Mol Physiol. 2009;297:L388-400 pubmed publisher
Shahrara S, Pickens S, Dorfleutner A, Pope R. IL-17 induces monocyte migration in rheumatoid arthritis. J Immunol. 2009;182:3884-91 pubmed publisher
Yammani R, Long D, Loeser R. Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes. Arthritis Rheum. 2009;60:792-800 pubmed publisher
Mizutani K, Sud S, Pienta K. Prostate cancer promotes CD11b positive cells to differentiate into osteoclasts. J Cell Biochem. 2009;106:563-9 pubmed publisher
Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, et al. Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment. J Exp Med. 2009;206:249-58 pubmed publisher
Bendrik C, Dabrosin C. Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo. J Immunol. 2009;182:371-8 pubmed
Osei Kumah A, Smith R, Clifton V. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Cytokine. 2008;43:187-93 pubmed publisher
Bonecchi R, Borroni E, Anselmo A, Doni A, Savino B, Mirolo M, et al. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation. Blood. 2008;112:493-503 pubmed publisher
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Yang H, Cohen P, Rousseau S. IL-1beta-stimulated activation of ERK1/2 and p38alpha MAPK mediates the transcriptional up-regulation of IL-6, IL-8 and GRO-alpha in HeLa cells. Cell Signal. 2008;20:375-80 pubmed
Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler Thromb Vasc Biol. 2008;28:135-41 pubmed
Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, et al. S100A11, an dual mediator for growth regulation of human keratinocytes. Mol Biol Cell. 2008;19:78-85 pubmed
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
Watanabe K, Bianco C, Strizzi L, Hamada S, Mancino M, Bailly V, et al. Growth factor induction of Cripto-1 shedding by glycosylphosphatidylinositol-phospholipase D and enhancement of endothelial cell migration. J Biol Chem. 2007;282:31643-55 pubmed
Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy P, Lampertico P, et al. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage. J Exp Med. 2007;204:667-80 pubmed
Fitzgerald D, Meade K, McEvoy A, Lillis L, Murphy E, MacHugh D, et al. Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells. Vet Immunol Immunopathol. 2007;116:59-68 pubmed
Fan X, Patera A, Pong Kennedy A, Deno G, Gonsiorek W, Manfra D, et al. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J Biol Chem. 2007;282:11658-66 pubmed
Johnson L, Clasper S, Holt A, Lalor P, Baban D, Jackson D. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J Exp Med. 2006;203:2763-77 pubmed
Smith C, Damås J, Otterdal K, Øie E, Sandberg W, Yndestad A, et al. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J Am Coll Cardiol. 2006;48:1591-9 pubmed
Hajjar A, Harvey M, Shaffer S, Goodlett D, Sjostedt A, Edebro H, et al. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect Immun. 2006;74:6730-8 pubmed
Daryadel A, Grifone R, Simon H, Yousefi S. Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha. J Biol Chem. 2006;281:27653-61 pubmed
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck L, et al. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin Immunol. 2006;120:220-31 pubmed
Li Y, Fan J, Chen M, Li W, Woodley D. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. J Invest Dermatol. 2006;126:2096-105 pubmed
Bersinger N, von Roten S, Wunder D, Raio L, Dreher E, Mueller M. PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis. Am J Obstet Gynecol. 2006;195:103-8 pubmed
Rhodes K, Raivich G, Fawcett J. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience. 2006;140:87-100 pubmed
Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, et al. Novel antiviral activity of chemokines. Virology. 2006;350:484-92 pubmed
Wunder D, Mueller M, Birkhäuser M, Bersinger N. Increased ENA-78 in the follicular fluid of patients with endometriosis. Acta Obstet Gynecol Scand. 2006;85:336-42 pubmed
Ramachandran R, Morice A, Compton S. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Am J Respir Cell Mol Biol. 2006;35:133-41 pubmed
Edwards M, Johnson M, Johnston S. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells. Am J Respir Cell Mol Biol. 2006;34:616-24 pubmed
Trevino J, Summy J, Gray M, Nilsson M, Lesslie D, Baker C, et al. Expression and activity of SRC regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Res. 2005;65:7214-22 pubmed
Mihara K, Smit M, Krajnc Franken M, Gossen J, Rooseboom M, Dokter W. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice. Eur J Immunol. 2005;35:2573-82 pubmed
Kim K, Burkhart K, Chen P, Frevert C, Randolph Habecker J, Hackman R, et al. Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice. Am J Respir Cell Mol Biol. 2005;33:271-9 pubmed
Holst R, Mattsby Baltzer I, Wennerholm U, Hagberg H, Jacobsson B. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery. Acta Obstet Gynecol Scand. 2005;84:551-7 pubmed
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, et al. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J Immunol. 2004;173:6592-602 pubmed
Nagineni C, Kutty V, Detrick B, Hooks J. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J Cell Physiol. 2005;203:35-43 pubmed
Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, et al. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases. Blood. 2005;105:830-7 pubmed
Kao C, Chen Y, Thai P, Wachi S, Huang F, Kim C, et al. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J Immunol. 2004;173:3482-91 pubmed
Phillips T. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis. 2004;25:1652-9 pubmed
Traves S, Smith S, Barnes P, Donnelly L. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J Leukoc Biol. 2004;76:441-50 pubmed
Bengtsson A, Ryan E, Giordano D, Magaletti D, Clark E. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood. 2004;104:1404-10 pubmed
Copeland S, Siddiqui J, Remick D. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J Immunol Methods. 2004;284:99-106 pubmed
Doubrovina E, Doubrovin M, Vider E, Sisson R, O Reilly R, Dupont B, et al. Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma. J Immunol. 2003;171:6891-9 pubmed
Keane M, Donnelly S, Belperio J, Goodman R, Dy M, Burdick M, et al. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. J Immunol. 2002;169:6515-21 pubmed
Hurst S, McLoughlin R, Monslow J, Owens S, Morgan L, Fuller G, et al. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J Immunol. 2002;169:5244-51 pubmed
Banisadr G, Queraud Lesaux F, Boutterin M, Pelaprat D, Zalc B, Rostene W, et al. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain. J Neurochem. 2002;81:257-69 pubmed
Huber T, Reinhardt H, Exner M, Burger J, Kerjaschki D, Saleem M, et al. Expression of functional CCR and CXCR chemokine receptors in podocytes. J Immunol. 2002;168:6244-52 pubmed
Humes H, Fissell W, Weitzel W, Buffington D, Westover A, MacKay S, et al. Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells. Am J Kidney Dis. 2002;39:1078-87 pubmed
Böttcher M, Jenmalm M, Bjorksten B, Garofalo R. Chemoattractant factors in breast milk from allergic and nonallergic mothers. Pediatr Res. 2000;47:592-7 pubmed
image
image 1 :
R&D Systems 208-IL-010/CF image 1
Recombinant Human IL-8/CXCL8 Protein Bioactivity
product information
brand :
R&D Systems
master code :
208-IL/CF
SKU :
208-IL-010/CF
product name :
Recombinant Human IL-8/CXCL8 Protein, CF
description :
The Recombinant Human IL-8/CXCL8 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human IL-8/CXCL8 Protein, CF has been validated for the following applications: Bioactivity.
target :
IL-8/CXCL8
category :
Proteins and Enzymes
sizes available :
10 ug (also 50 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
theoretical molecular weight :
8 kDa
gene symbol :
CXCL8
details of functionality :
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2. The ED50 for this effect is 0.5-2.5 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P10145.1
applications :
Bioactivity
source :
E. coli-derived Recombinant Human IL-8/CXCL8 Protein, CF
USD :
172
USD 2023 :
172 USD
alt names :
3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP1, GCP-1TSG-1, IL8, interleukin 8, K60, LAI, LECT, LUCT, LYNAP, MDNCF, MDNCFb-ENAP, member 8, MONAP, MONAPGCP1, NAF, NAP1, NAP-1NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.